Center for Biosystems

Center for sensing technologies

Project aim: The focus of the project is development and testing of the innovative low-cost sensors for monitoring of biomass and metabolites generated by the cells, that could potentially accumulate in the media and cause negative effects.
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About the project: Nowadays, Cellular Agriculture is becoming one of the main scientific topics dealing with the problem of producing food sustainably, contributing to the reduction of climate change, and addressing other environmental challenges. Cultivated meat production and its distribution in supermarkets and on our tables is one of the promising solutions that scientists are dealing with for this purpose. 
From 2019 BioSense Institute participates in this race of cultivated meat production, receiving The Good Food Institute competitive Grant &#8211; RealSense1, led by Dr Ivana Gadjanski.
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REALSENSE1 utilizes the lab-on-a-chip approach i.e. microfluidic bioreactors, which enable a significant reduction in time and cost of bioprocess development due to the high degree of control over process variables enabled by the laminar fluid flow in the microfluidic chips, as well as due to the small quantities of reagents needed for the microbioreactors.

Senior research associate in the field of natural/mathematical sciences - biology

Ivana Gađanski

Dr Radonic received a PhD in electronics in 2010 at University of Novi Sad. He authored and co-authored 2 book chapters, 30 journal papers, more than 60 conference papers, 10 technical solutions, and has more than 440 citations. Dr Radonić was the project leader in four Serbian national projects, one project financed by European Bank for Research and Development, one Bilateral project between Serbia and Slovenia, project financed by Good Food Institute, USA and WP leader in one FP7 project–InnoSense and one H2020 project–ANTARES. He participates in a number of research FP6, FP7, H2020, COST, EUREKA, IPA projects. He is a member of IEEE and MAGEO (Microwaves in Agriculture, Environment and Earth Observation) groups and was a member of Technical Program Committees of EUROCON (Computer as Tool, 2015) conference, Salamanca, Spain and Sensordevices conference 2019, Nice, France. Steering committee member of the BioSense Institute 2015-2020, and the vice president of Scientific council of the BioSense Institute 2015-2020. From 2022, he is the assistant director for science. Reviewer in different scientific journals: Scientific Reports, Lab-on-Chip, IEEE Sensors Journal, MDPI Sensors, MDPI Electronics, Sensors and Actuators B, IEEE Wireless and component letters, Electronic letters, etc. The main expertise of Dr Radonic is microfluidics, sensors development (microwave, optical, microfluidic sensors and biosensors), electronic, RF and microwave passive components (filters, coupler, diplexer, etc.), antennas and RFID systems, periodical and artificial materials, fabrication technologies, measurements and characterization.

Vasa Radonić

Stefan Jarić is employed at the Center for Sensing Technologies as a Research Assistant and a final-year PhD student in materials physics at the Department of Physics, Faculty of Sciences at the University of Novi Sad. Stefan works on the development and research of new solutions in the field of biosensors based on graphene field effect transistors and specific synthetic receptors (aptamers) for the detection of various analytes in the area of environmental protection (toxins) and medicine (biomarkers). His expertise is developing in the direction of micro and nanofabrication of sensor platforms (photolithography, physical vapor deposition of materials), characterization of materials (Raman spectroscopy, atomic force microscopy) and electrical characteristics of biosensors.

Stefan Jarić

Dr. Nikola Knežević is a Full research professor at the Center for Sensor Technologies at the Biosense Institute. He received his doctorate in 2009 at Iowa State University, USA, in synthesizing and functionalizing porous silica nanoparticles for stimuli-responsive and targeted drug delivery. He gained further research experience as a postdoctoral fellow at the University of Houston (research in the field of multistep organic synthesis), Universidad Complutense de Madrid (nanomaterials science), Institute Charles Gerhardt Montpellier (nanomaterials science), FP7 - ERA ERA Chairs postdoctoral fellow at the Vinča Nuclear Institute and as Pole Chimie Ballard Visiting Professor at the University of Montpellier. Nikola is the author/co-author of over 40 scientific publications, including 2 book chapters and one patent, with over 1900 citations and an H index of 21. Nikola is the coordinator of one H2020 project (NANOFACTS GA 952259), three Serbian national projects (PRECAST – 6060755, proof of concept – 5566, technology transfer -1135), and two bilateral research projects (with France and Germany). Research interests: Synthesis of functional bioresponsive nanomaterials for use in health protection and construction of biosensors.

Nikola Knežević

Ivana Podunavac is a Junior Researcher at BioSense Institute and PhD student of Applied Physics at the Faculty of Sciences, University of Novi Sad, Serbia. Her fields of research are microfluidics,
simulations, fabrication and testing of sensing devices.

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Ivana Podunavac

Teodora Knežić joined BioSense Institute as Junior Researcher in the Center for Biosystems. Teodora has a bachelor's and master's degrees in Molecular Biology and she is currently a Ph.D. student of Molecular Biology at the Faculty of Sciences, University of Novi Sad, Serbia, with Dr Ivana Gađanski as the mentor. During her studies she volunteered in the Laboratory for Biochemistry and Molecular Biology at the Faculty of Sciences where was involved in the analyses of gene expression of the European corn borer using techniques for RNA isolation, gel electrophoresis, cDNA synthesis, end-point and qPCR analysis. Also, at the Institute for Nuclear Sciences Vinča she was trained to work with human cancer cell cultures, perform viability assays and flow cytometry. Teodora has just completed a 3 month specialization practice in BioSense as well. Her main research interests are biosensor development and molecular techniques for pathogen detection, cellular agriculture and development of lab-on-a-chip platforms.

Teodora Knežić

Mila Djisalov has a BSc in Biochemistry and a Master’s degree in Molecular Biology. She is currently a Ph.D. Student of Biochemistry at the Faculty of Sciences in Novi Sad and a Junior Researcher in BioSense Institute. Her main research interests are biosensor development and molecular tools for pathogen detection, cellular agriculture, and the development of lab-on-a-chip platforms. Mila is also one of the co-founders of the student group The BioSense Alt. Protein Project formed at the BioSense Institute.

MIla Đisalov

Ivana Gadjanski PhD is Research Associate Professor at BioSense Institute, University of Novi Sad, Serbia. She graduated from the Faculty of Biology, University of Belgrade as the 1st in the class (2003). She obtained PhD in Neuroscience (summa cum laude -oral presentation) from Georg-August University &amp; Max Planck Institute for Biophysical Chemistry, Goettingen, Germany (2007) and worked as a postdoctorate researcher at Ludwig-Maximillian University, Munich, Germany. Ivana then turned to the bioengineering field and spent a year as a Fulbright Visiting Scholar at Columbia University, USA - Lab for Stem cells and Tissue Engineering (Head: prof. Gordana Vunjak-Novakovic). In BioSense, she is moving further into the cellular agriculture field - developing solutions for cultivated meat. She also works on biosensing for agrifood, biomedicine and environmental monitoring. Ivana is a grantee of the Good Food Institute and coordinator of Horizon2020 projects DRAGON GA 810755 and IPANEMA GA 872662.

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RealSense1 i2 (1) 1

THE GOOD FOOD INSTITUTE GRANTS

BioSense participates in a range of pan-European initiatives that aim to centralise environmental data coming from various regions, sources and institutions. Such a database would provide researchers, government institutions and NGOs precious information about the state of ecosystems around Europe along with an insight on how they are changing in time.

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Environmental and ecological data is very heterogeneous. It encompasses logs from sensors, expert observations, photos from smartphones and UAVs, lab analyses and others. Individual data from one source or one region provides little information, as it cannot be analysed in a wider context. Also, data is usually kept at individual institutions separately, which poses further hurdles for comprehensive analysis and directly contradicts the EU principles of open science.

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For these reasons, we participate in a suite of European projects where we are developing a platform for deposition of environmental and ecological data. The platforms facilitates networking between European institutions and a collaborative approach to tackling the problems of climate change, global warming, habitat loss and altered competitive relationships within flora and fauna.

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<img class="alignnone wp-image-1847 size-full" src="https://api.biosens.rs/wp-content/uploads/2021/07/GIS.png" alt="" width="940" height="475" />

<img class="alignnone wp-image-1848 size-full" src="https://api.biosens.rs/wp-content/uploads/2021/07/GIS-1.png" alt="" width="940" height="477" />

<img class="alignnone wp-image-1849 size-full" src="https://api.biosens.rs/wp-content/uploads/2021/07/GIS-2.png" alt="" width="850" height="530" />

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Ecology Platforms

Due to their small diameter (ca. 100 nm) and highly porous nature, mesoporous silica nanoparticles (MSNs) have high specific surface area (ca. 1000 m2/g) which can be utilized for a plethora of functionalizations, loading/adsorption of different molecules and their removal, or as a platform for signaling molecules to detect different analytes.

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Mesoporous organosilicate nanomaterial (also termed Periodic Mesoporous Organosilica (PMO)) contains a large percentage of organic groups within the nanosphere, exhibiting unique characteristics for different applications.

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Our research is being conducted toward design of new series of MSN and PMO nanomaterials, as well as their core/shell analogs.

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Surface of these nanomaterials is being modified with different molecules to specifically interact with desired tissues, pollutants, or analytes, for their efficient application in monitoring and protection of health, food and the environment.

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One of the research goals is to develop smart nanomaterials with stimuli-responsive release of MSN/PMO-loaded molecules for diagnostics, imaging, and therapy of cancer tissues.

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Polymer-MSN composite materials are also being developed for applications as antimicrobial smart food packaging and medical implants.

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Finally, PMO nanoparticles are being constructed for efficient UVA and UVB blocking and application in a safer generation of sunscreens.

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<img class="alignnone wp-image-1626 size-full" src="https://api.biosens.rs/wp-content/uploads/2021/06/slika-1.png" alt="" width="837" height="466" />

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<a href="https://api.biosens.rs/wp-content/uploads/2021/06/68.tif"><img class="alignnone size-medium wp-image-1526" src="https://api.biosens.rs/wp-content/uploads/2021/06/68.tif" alt="" width="1" height="1" /></a>

Projects:

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PRECAST: Prediction of Cancer Treatment Effectiveness with Stimuli-responsive Nanomaterials, Science Fund of the Republic of Serbia

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<a href="https://cordis.europa.eu/project/id/952259">NANOFACTS: Networking Activities for Nanotechnology-Facilitated Cancer Theranostics H2020-WIDESPREAD-2020-5 </a>

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Novel Active Components for Health- and Environment-friendly UV-Blocking Based on Enriched Organosilica Nanomaterials, Project ID: 5566, The Innovation Fund of the Republic of Serbia

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III 44006, Ministry of Education, Science, and Technological Development of the Republic of Serbia.

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Silica nanomaterials

Biosensors are analytical devices for detection of the biologic material which may include biomolecules, biological structures or microorganisms (bacteria, viruses, algae, spores). Nowadays, they are being used for food safety evaluation, environmental monitoring, or for early detection of infectious diseases in biomedicine.

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We work on the development of new point-of-need biosensor transducers, biofunctionalization of the sensors with bioreceptors, and sensor integration into microfluidic systems. Developed biosensors are being implemented for detection of bacteria in food and water, detection of cyanobacterial toxins produced during harmful algal blooms, as well as for a fast detection of faecal bacteria and other microbiological pathogens in water.

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More concrete, we focus our research on development of new nanomaterials for functionalization of the biosensor electrodes and fabrication of new electrochemical transducers using different fabrication technologies. Particular attention is dedicated to new materials which will enable selective and efficient binding of the analytes and to the novel biosensor designs to increase the selectivity, sensitivity and limit of detection. Therefore, we develop the novel methods of graphene modification, and the mask-less methods of graphene patterning and integration to conventional industrial routes.

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A special focus is placed on biosensors containing enzymes, antibodies and nucleic acids (DNA, RNA) as recognition elements. This research is performed in close collaboration with the Center for Biosystems in which experts work on biomolecular engineering and molecular detection methods including the cutting-edge isothermal methods for nucleic acid amplification such as LAMP (loop-mediated isothermal amplification) and RPA (recombinase polymerase amplification) and other ways of molecular detection which can be well combined with the biosensing methods.

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In addition, BioSense Institute works on the new types of biosensors for monitoring nutrients and products of cellular metabolism during cultured meat production, as another example of excellent multidisciplinary collaboration with the Center for BioSystems and the researchers working on Cellular agriculture topics.

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<img class="alignnone wp-image-1508 " src="https://api.biosens.rs/wp-content/uploads/2021/06/58.png" alt="" width="701" height="417" />

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Collaborative projects with the Center for Biosystems:

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<a href="http://www.ipanema2020.com/">H2020 MSCA-RISE IPANEMA GA 872662</a> - Integration of PAper-based Nucleic acid testing mEthods into Microfluidic devices for improved biosensing Applications

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<a href="http://www.realsense.rs/">REALSENSE1</a> - Monitoring of cell culture parameters using sensors for biomass and nutrients/metabolites in media: Lab-on-a-Chip (LOC) approach, 2019-2021; Good Food Institute Inaugural Competitive Grant Program.

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<a href="http://www.realsense.rs/">REALSENSE2</a> – From lab-on-a-chip to custom bioreactor: scale up modeling study, 2020-2022 Good Food Institute Competitive Grant Program

Biosensors

Microfluidics deals with manipulation and control of small quantity of fluids in micro- and nano-channels and as such, it initiated revolution in different fields including microbiology, medicine, and chemical analysis.

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We focus our research on analysis and design of different microfluidic components, development of the new materials for microfluidic chips, and integration of the microfluidics with different detection systems and electronics. To that end, we employ different fabrication technologies PDMS, LTCC, 3D and inkjet printing, bio-printing, xurography, and hybrid technologies.

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Since the utilization of only one microfabrication technology cannot fulfill all the requirements set by the microfluidic chip, we also employ hybrid fabrication approach, i.e. we combine various technologies with various materials to realize microfluidic chips with high resolution, small dimension, and high flow rate. For instance, hybrid approach is used to develop multilayered filtration units, high flow rate mixers, as well as pollen separation pinch flow.

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Also, we integrate microfluidic concept and reagent storages with various transduction principles and sensors, to develop lab-on-a-chip platform which finds application in in-field environment monitoring, biomedicine and food analysis.

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Moreover, we work on development of microfluidic reactor that integrates cell culture system, different sensors for nutrient concentration and metabolic waste products measurement, optical detection system and electronics into single chip.

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Our efforts are also focused on medical applications - we combine the top notch microfluidic technologies and organ-on-a-chip concept, along with the technology of induced pluripotent stem cells, to design a new generation of heart-on-a-chip for the patient treatment.
In addition, we develop a microfluidic platform able to reproduce the dynamic and heterogeneity of the soil conditions while implementing monitoring systems for investigating the root/plant–microbiome interactions in a microfluidic device.

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<img class="alignnone wp-image-1529 size-full" src="https://api.biosens.rs/wp-content/uploads/2021/06/70.jpg" alt="" width="763" height="323" />

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PROJECTS:

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CISTEM - Heart On chip based on induced pluripotent Stem cell Technology for personalized Medicine, H2020-MSCA-RISE-2017, GA No. 778354

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REALSENSE1 - Monitoring of cell culture parameters using sensors for biomass and nutrients/metabolites in media: Lab-on-a-Chip (LOC) approach, 2019-2021; Good Food Institute Inaugural Competitive Grant Program.

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IPANEMA - Integration of PAper-based Nucleic acid testing mEthods into Microfluidic devices for improved biosensing Applications, H2020-MSCA-RISE-2019, 2020-2024. GA No. 872662,

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NANOFACTS - Networking Activities for Nanotechnology-Facilitated Cancer Theranostics, H2020 - WIDESPREAD-05-2020 – Twinning, 2021-2024. GA No. 952259

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Development and Integration of Microfluidic Biosensors for Meat Safety monitoring in farm-to-slaughterhouse continuum (DIBMES), Innovation fond, Serbia, Call: Proof of Concept ID: 5524

Microfluidics

Cellular agriculture refers to production of agricultural products using cell cultures. Cellular agriculture products are part of the larger group called Alternative proteins (alt.pro). Alt.pro are proteins that can act as substitutes for traditional animal‐based food. Generally, alt.pro are classified based on the source into: Plant-based, Insect-based and Cultured meat that is produced by cellular agriculture methods. Majority of the methods for cellular agriculture stem from the tissue engineering and biomedical field, while the application of the products generated by cellular agriculture falls predominantly into the food science.

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Cultured meat is the main type of alternative proteins BioSense addresses. One of the main challenges for scaling up the cultured meat production to the industry levels is concerning the overall costs of production. The highest portion of costs (up to 80%) for cultured meat production is for the cell culture medium and fetal calf or bovine serum. The challenge to decrease expenses is not only in designing low-cost cell culture medium formulations, but also in developing methods to optimize the bioreactor design, instrumentation and to include monitoring features via sensors for the maximum cell production capacity per unit medium volume.

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In that sense, we focus our research on optimizing procedures for generating cellular products, and we closely collaborate with the Center for sensing technologies regarding development of sensors for monitoring of key parameters in cultured meat bioprocess: Biomass concentration, a key variable in cell cultures and Concentration of key nutrients – glucose and glutamine and their main metabolites, lactate and ammonia, that can inhibit the cell growth. Sensors are tested using "miniaturized" bioreactors i.e. microfluidic model and Lab-on-a-chip (LOC) principle, which will be scaled up to the bioreactors of larger volumes.

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Projects:

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REALSENSE1 - Monitoring of cell culture parameters using sensors for biomass and nutrients/metabolites in media: Lab-on-a-Chip (LOC) approach, 2019-2021; Good Food Institute Inaugural Competitive Grant Program.

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REALSENSE2 - From lab-on-a-chip to custom bioreactor: scale up modeling study, 2020-2022 Good Food Institute Competitive Grant Program

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<img class="alignnone wp-image-1459 size-large" src="https://api.biosens.rs/wp-content/uploads/2021/06/31-1024x644.png" alt="" width="1024" height="644" />

Cellular agriculture

REALSENSE1 – Monitoring of cell culture parameters using sensors for biomass and nutrients/metabolites in media: Lab-on-a-Chip (LOC) approach, 2019-2021; Good Food Institute 2018 Competitive Grant Program

Centar za Biosisteme

Centar za senzorske tehnologije

Cilj projekta: Razvijanje i testiranje inovativnih i ekonomičnih senzora za biomasu i za različite metabolite koji se generišu tokom gajenja ćelija i mogu se akumulirati u medijumu, što može dovesti do negativnog efekta na ćelije.
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O projektu: Ćelijska poljoprivreda se u današnje vreme pominje kao jedna od vodećih naučnih tema koje se bavi problemom održive proizvodnje hrane, doprinoseći na taj način smanjenju klimatskih promena, kao i rešavanju drugih izazova koji se tiču zaštite životne sredine. Proizvodnja kultivisanog mesa i njegova distribucija u supermarkete i na naše trpeze, jedno je od mogućih rešenja kojima se naučnici bave kako bi rešili neke od pomenutih problema.
Od 2019 godine, BioSens Institut učestvuje u toj “trci” proizvodnje kultivisanog mesa, dobijanjem granta Instituta Dobre Hrane &#8211; RealSense1 &#8211; kojim koordinira dr Ivana Gađanski.
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REALSENSE1 projekat koristi pristup tzv. “laboratorije na čipu” tj. gajenje ćelija u mikrofluidičnim bioreaktorima, koji omogućuju značajno smanjenje vremena i troškova razvoja bioprocesa zbog visokog stepena kontrole, omogućenog laminarnim kretanjem fluida, kao i malim količinama potrebnih reagenasa, što je omogućeno dimenzijama mikrofluidičnih bioreaktora.

 Viši naučni saradnik u oblasti prirodno-matematičke nauke - biologija

Dr Radonić je doktorirao iz oblasti elektronike 2010. godine na Univerzitetu u Novom Sadu. Autor je 2 poglavlja u knjizi, 30 radova u naučnim časopisima, više od 60 konferencijskih radova, 10 tehničkih rešenja i ima više od 440 citata. Dr Radonić je bio rukovodilac na četiri nacionalna projekta, jednom projektu koji je finansirala Evropska banka za istraživanje i razvoj, jednom bilateralnom projektu između Srbije i Slovenije, projektu koji je finansirao Good Food Institute iz SAD-a i vođa radnog paketa u jednom FP7 projektu – Innosens i jednom H2020 – Antares. Učestvovao je u brojnim istraživačkim projektima iz programa FP6, FP7, H2020, COST, Eureka, IPA. Član je grupa IEEE i MAGEO (Mikrotalasi u poljoprivredi, životnoj sredini i posmatranju Zemlje) i član tehničkih programskih odbora konferencije EUROCON (Computer as a Tool, 2015), Salamanka, Španija i Sensodevice konferencije 2019, Nica, Francuska. Član upravnog odbora Instituta BioSense od 2015-2020 i potpredsednik Naučnog veća Instituta BioSense 2015-2020. Od 2022 obavlja funkciju pomoćnika direktora za nauku. Recenzent u mnogim naučnim časopisima: Scientific Reports, Lab-on-Chip, IEEE Sensors Journal, MDPI Sensors, MDPI Electronics, Sensors and Actuators B, IEEE Wireless and component letters, Electronic letters, etc. Oblast naučnog istraživanja dr Radonića je mikrofluidika, razvoj senzora (mikrotalasni, optički, mikrofluidni senzori i biosenzori), elektronskih, RF i mikrotalasnih pasivnih komponenti (filteri, spojnica, diplekser itd.), antena i RFID sistema, periodični i veštački materijali, tehnologije fabrikacije, merenja i karakterizacija.

Stefan Jarić je zaposlen u Centru za senzorske tehnologije kao istraživač-saradnik i student završne godine doktorskih studija fizike materijala na Departmanu za fiziku, Prirodno-matematičkog fakulteta u Novom Sadu. Stefan se bavi razvojem i istraživanjem novih rešenja na polju biosenzora zasnovanih na grafenskim tranzistorima sa efektom polja i specifičnih sintetičkih receptora (aptamera) za detekciju raznih analita iz sfere zaštite životne sredine (toksini) i medicine (biomarkeri). Njegova ekpertiza se razvija u pravcu mikro i nanofabrikacije senzorskih platformi (fotolitografija, fizičko naparavanje materijala), karakterizacije materijala (Ramanova spektroskopija, mikroskopija atomskih sila) i električnih karakteristika biosenzora.

Dr Nikola Knežević je Naučni savetnik u Centru za senzorske tehnologije na Institutu Biosens. Doktorirao je 2009. godine na Iowa State University, SAD, iz oblasti sinteze i funkcionalizacije poroznih silikatnih nanočestica za stimulans-responsivnu i ciljajuću isporuku lekova. Dalje istraživačko iskustvo stekao je kao postdoktorand na Univerzitetu u Hjustonu (istraživanje iz oblasti višestepene organske sinteze), Universidad Complutense de Madrid (nauka o nanomaterijalima), Institute Charles Gerhardt Montpellier (nauka o nanomaterijalima), FP7 – ERA ERA Chairs postdoktorand u Nuklearnom institutu Vinča i kao Pole Chimie Balard gostujući profesor na Univerzitetu u Monpeljeu. Nikola je autor/koautor na preko 40 naučnih publikacija, uključujući 2 poglavlja u knjigama i jedan patent, sa preko 1900 citata i H indeksom 21. Nikola je koordinator jednog projekta H2020 (NANOFACTS GA 952259), tri srpska nacionalna projekta (PRECAST – 6060755, dokaz koncepta – 5566, transfer tehnologija -1135) i dva bilateralna istraživačka projekta (sa Francuskom i Nemačkom). Istraživačka interesovanja: sinteza funkcionalnih bioresponsivnih nanomaterijala za primenu u zaštiti zdravlja i konstrukciji biosenzora.

Ivana Podunavac je istraživač pripravnik na Institutu BioSens i student doktorskih studija primenjene fizike na Prirodno-matematičkom fakultetu, Univerziteta u Novom Sadu. Njene oblasti istraživanja su mikrofluidika, kao i izrada i testiranje senzora.

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Teodora Knežić je zaposlena kao istraživač-pripravnik na Institutu BioSens, u Centru za biosisteme. Teodora je završila osnovne i master akademske studije molekularne biologije i trenutno pohađa doktorske akademske studije molekularne biologije na Prirodno-matematičkom fakultetu Univerziteta u Novom Sadu, pod mentorstvom dr Ivane Gađanski. Tokom svojih studija volontirala je u Laboratoriji za biohemiju i molekularnu biologiju na Prirodno-matematičkom fakultetu gde je vršila analizu genske ekspresije kukuruznog plamenca koristeći metode za izolaciju RNK, gel elektroforezu, sintezu cDNK, end-point i qPCR analizu. Takođe, na Institutu za nuklearne nauke Vinča obučena je za rad sa humanim ćelijskim linijama, izvođenje eseja vijabilnosti i protočnu citometriju. Teodora je završila i tromesečno fakultativno stručno usavršavanje na Institutu BioSens. Njen glavni fokus istraživanja je razvoj biosenzora i molekularnih tehnika za detekciju patogena, ćelijska poljoprivreda i razvoj lab-on-a-chip platformi.

Mila Đisalov je diplomirani biohemičar i master molekularne biologije. Trenutno je student doktorskih studija biohemije na Prirodno-matematičkom fakultetu u Novom Sadu i istraživač-pripravnik na Institutu BioSens. Njena glavna istraživačka interesovanja vezana su za razvoj biosenzora i molekularnih alata za detekciju patogena, ćelijska poljoprivreda i razvoj laboratorija na čipu. Mila je takođe jedan od osnivača studentske grupe The BioSense Alt. Protein Project, osnovane na Institutu BioSens.

Mila Đisalov

Dr Ivana Gađanski je viši naučni saradnik na Institutu Biosens, Univerzitet u Novom Sadu, Srbija. Diplomirala je na Biološkom fakultetu Univerziteta u Beogradu kao prva u klasi (2003). Doktorirala je iz neuronauka (ocena summa cum laude – usmena odbrana) na Georg-August Univerzitetu i Institutu za biofizičku hemiju Maks Plank, Getingen, Nemačka (2007) i radila na posledoktorskim studijama na Univerzitetu Ludvig-Maksimilijian, Minhen, Nemačka. Ivana se zatim posvetila oblasti bioinženjerstva i provela godinu dana kao Fulbrajt stipendista – gostujući istraživač na Univerzitetu Kolumbija, Njujork, SAD u Laboratoriji za matične ćelije i tkivno inženjerstvo (rukovodilac: prof. Gordana Vunjak-Novaković). U BioSensu radi na bioinženjerstvu u oblasti ćelijske poljoprivrede - razvija rešenja za kultivisano meso. Takođe radi na razvoju biosenzora za monitoring u oblasti poljoprivrede, biomedicine i zaštite životne sredine. Ivana je stipendista Instituta za dobru hranu i koordinator Horizont2020 projekata DRAGON GA 810755 i IPANEMA GA 872662.

GRANTOVI INSTITUTA DOBRE HRANE

Ćelijska poljoprivreda se bavi proizvodnjom poljoprivrednih proizvoda pomoću kultura ćelija i ulazi u oblast tzv. alternativnih proteina (alt.pro). Alt. pro su proteini koji se mogu koristiti kao zamena za uobičajene životinjske proteine. Alt.pro mogu biti biljnog porekla, mogu biti i od insekata, a posebna grupa proteina označena kao kultivisano meso se dobija pomoću metoda ćelijske poljoprivrede. Većina metoda ćelijske poljoprivrede potiče iz biomedicine i tkivnog inženjerstva, dok primene i upotreba proizvoda dobijenih ćelijskom poljoprivredom pretežno pripadaju oblasti tehnologije hrane.

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Kultivisano meso je osnovni tip alternativnih proteina kojim se bavi Institut BioSens. Jedan od glavnih izazova za skaliranje tj. industrijsku proizvodnju kultivisanog mesa su ukupni troškovi proizvodnje. Najveći deo troškova (čak do 80%) za kultivaciju mesa potiče od cene hranljivog medijuma i fetalnog goveđeg seruma, neophodnih za gajenje ćelija u kulturi. Troškovi se mogu smanjiti ne samo jeftinijim formulacijama hranljivog seruma, već i putem metoda optimizacije dizajna bioreaktora i instrumenata i naročito upotrebom senzorskog sistema praćenja ključnih parametara, da bi se postigla maksimalna produkcija ćelija po jedinici zapremine medijuma.

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U tom smislu se fokusiramo na optimizaciju procedura za dobijanje ćelijskih produkata, a u bliskoj saradnji sa Centrom za senzorske tehnologije razvijamo i senzore za praćenje ključnih parametara u bioprocesu kultivacije mesa: koncentracija biomase, što je osnovni parametar ćelijske kulture i koncentracija osnovnih hranljivih materija – glukoze i glutamina i njihovih glavnih metabolita, laktata i amonijaka, koji mogu inhibirati rast ćelija. Senzori se testiraju na „minijaturizovanim“ bioreaktorima tj. mikrofluidnim modelima uz primenu „Laboratorija na čipu“ principa, koji će se zatim skalirati na bioreaktore većih zapremina.

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Projekti:

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REALSENSE1 - Monitoring of cell culture parameters using sensors for biomass and nutrients/metabolites in media: Lab-on-a-Chip (LOC) approach, 2019-2021; Good Food Institute Inaugural Competitive Grant Program.

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REALSENSE2 - From lab-on-a-chip to custom bioreactor: scale up modeling study, 2020-2022 Good Food Institute Competitive Grant Program

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<img class="alignnone wp-image-1459 size-large" src="https://api.biosens.rs/wp-content/uploads/2021/06/31-1024x644.png" alt="" width="1024" height="644" />

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Ćelijska poljoprivreda

Biosenzori su analitički uređaji koji služe za detekciju biološkog materijala kao što su biomolekuli, biološke strukture ili mikroorganizmi (bakterije, virusi, alge, spore). Široku primenu biosenzori nalaze u kontroli bezbednosti hrane, monitoringu životne sredine, kao i u ranom otkrivanju infektivnih bolesti u biomedicini.

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Naša istraživanja su usmerena na razvoj novih biosenzorskih transdjusera, biofunkcionalizaciju senzora sa bioreceptorima, i integraciju senzora u mikrofluidične sisteme. Razvijeni senzori se koriste za detekciju bakterija u hrani i vodi, za detekciju cijano toksina nastalih tokom cvetanja algi, kao i za brzu detekciju fekalnih bakterija i drugih mikrobioloških patogena u vodi.

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Bavimo se i razvojem novih nanomaterijala za funkcionalizaciju biosenzorskih elektroda i fabrikaciju novih elektrohemijskih biosenzora koristeći različite tehnologije fabrikacije. Deo istraživanja je usmeren na nove materijale koji će omogućiti selektivno i efikasno vezivanje analita, kao i na projektovanje biosenzora u cilju poboljšanja njihove selektivnosti, osetljivosti i limita detekcije. Bavimo se i novim metodama modifikacije grafena, novim metodama za njegovu sintezu i integraciju u konvencionalne industrijske procese.

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Posebna pažnja je posvećena biosenzorima na bazi enzima, antitela i nukleinskih kiselina (DNK, RNK). Ova istraživanja se izvode u bliskoj saradnji sa Centrom za biosisteme u kojem istraživači rade na metodama biomolekularnog inženjerstva i molekularne dijagnostike što uključuje i najsavremenije izotermalne metode za umnožavanje nukleinskih kiselina kao što su LAMP (Loop Mediated Isothermal Amplification) i RPA (Recombinase Polymerase Amplification) koje se mogu dobro kombinovati sa biosenzorima razvijenim u okviru Centra za senzorske tehnologije.

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Takođe, Institut BioSens radi na novim vrstama biosenzora za monitoring hranljivih materija i produkata metabolizma ćelija u procesu proizvodnje kultivisanog mesa, što je još jedan primer dobre multidisciplinarne saradnje sa Centrom za biosisteme i istraživačima koji rade u okviru oblasti ćelijske poljoprivrede.

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<img class="alignnone wp-image-1508 " src="https://api.biosens.rs/wp-content/uploads/2021/06/58.png" alt="" width="701" height="417" />

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Zajednički projekti sa Centrom za biosisteme:

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H2020 MSCA-RISE IPANEMA GA 872662 - Integration of PAper-based Nucleic acid testing mEthods into Microfluidic devices for improved biosensing Applications

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REALSENSE1 - Monitoring of cell culture parameters using sensors for biomass and nutrients/metabolites in media: Lab-on-a-Chip (LOC) approach, 2019-2021; Good Food Institute Inaugural Competitive Grant Program.

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REALSENSE2 – From lab-on-a-chip to custom bioreactor: scale up modeling study, 2020-2022 Good Food Institute Competitive Grant Program

Biosenzori

BioSens učestvuje u raznim panevropskim inicijativama kojima je cilj da centralizuju podatke o životnoj sredini koji dolaze iz različitih regiona, izvora i institucija. Takva baza podataka pružila bi istraživačima, vladinim institucijama i nevladinim organizacijama dragocene informacije o stanju ekosistema širom Evrope, kao i uvid u to kako se oni vremenom menjaju.

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Ekološki podaci su vrlo heterogeni. Obuhvataju zapise sa senzora, stručna zapažanja, fotografije sa pametnih telefona i dronova, laboratorijske analize i druge. Pojedinačni podaci iz jednog izvora ili jednog regiona pružaju malo informacija, jer se ne mogu analizirati u širem kontekstu. Takođe, podaci se obično čuvaju u pojedinačnim institucijama odvojeno, što predstavlja dodatne prepreke za sveobuhvatnu analizu i direktno je u suprotnosti sa principima otvorene nauke EU.

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Iz tih razloga učestvujemo u nizu evropskih projekata gde razvijamo platformu za čuvanje podataka o životnoj sredini i ekologiji. Platforme olakšavaju umrežavanje između evropskih institucija i zajednički pristup rešavanju problema klimatskih promena, globalnog zagrevanja, gubitka staništa i izmenjenih odnosa unutar flore i faune.

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<img class="alignnone wp-image-1847 size-full" src="https://api.biosens.rs/wp-content/uploads/2021/07/GIS.png" alt="" width="940" height="475" />

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<img class="alignnone wp-image-1848 size-full" src="https://api.biosens.rs/wp-content/uploads/2021/07/GIS-1.png" alt="" width="940" height="477" />

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<img class="alignnone wp-image-1849 size-full" src="https://api.biosens.rs/wp-content/uploads/2021/07/GIS-2.png" alt="" width="850" height="530" />

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Ekološke platforme

Mikrofluidika je oblast koja se bavi manipulacijom i kontrolom malih količina fluida u mikro- i nano-kanalima i kao takva mikrofludika je napravila pravu malu revoluciju u mikrobiološkim, medincinskim i hemijskim analizama.

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Naša istraživanja su fokusirana na analizu i projektovanje različitih mikrofludičinih komponenta, razvoj novih materijala za mikrofluidične čipove i integraciju mikrofluidike sa različitim sistemima za detekciju i elektronikom. U tu svrhu koristimo različite tehnologije fabrikacije, kao što su Polidimetilsiloksan (PDMS), niskotemperaturna zajedno pečena keramika (LTCC), 3D, inkdžet i bio-štampa, ksirografija, kao i hibridne tehnologije.
Kako korišćenje samo jedne tehnologije fabrikacije ne može da ispuni sve zahteve savremenih mikrofluidičnih sistema, koristimo hibridni pristup - kombinujemo različite tehnologije sa različitim materijalima da bismo dobili mikrofluidične čipove sa visokom rezolucijom, malim dimenzijama i visokim protocima. Na primer, hibridni pristup se koristi za razvoj višeslojnih čipova za filtraciju, mešača fluida za visoke protoke, kao i separatora za polen.

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Takođe, integrišemo mikrofluidne čipove sa reagentima za analizu na čipu sa različitim tipovima senzora u cilju razvoja labratorije na čipu za detekciju parametara fluida, koji nalaze primenu u kontroli mešanja fluida, biomedicine, monitoringu ekosistema i analizi tečne hrane.
Osim toga, radimo na razvoju mikrofluidičnog reaktora, koji integriše sistem za ćelijsku kulturu, različite senzore za detekciju koncentracije hranljivih materija i merenje metaboličkih otpadnih proizvoda, optičku detekciju i elektroniku u jedan jedinstveni sistem na čipu.

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Prateći ovaj koncept, razvijamo i mikrofluidičnu platformu koja ima mogućnost da reprodukuje dinamiku i heterogenost zemljišta na taj način razvijajući sistem za monitoring interakcije između biljke i mikrobioma.

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<img class="alignnone wp-image-1529 size-full" src="https://api.biosens.rs/wp-content/uploads/2021/06/70.jpg" alt="" width="763" height="323" />

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PROJEKTI:

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CISTEM - Heart On chip based on induced pluripotent Stem cell Technology for personalized Medicine, H2020-MSCA-RISE-2017, GA No. 778354

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REALSENSE1 - Monitoring of cell culture parameters using sensors for biomass and nutrients/metabolites in media: Lab-on-a-Chip (LOC) approach, 2019-2021; Good Food Institute Inaugural Competitive Grant Program.

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IPANEMA - Integration of PAper-based Nucleic acid testing mEthods into Microfluidic devices for improved biosensing Applications, H2020-MSCA-RISE-2019, 2020-2024. GA No. 872662,

Mikrofluidika

Zbog malog prečnika (oko 100 nm) i veoma porozne prirode, mezoporozne silikatne nanočestice (MSN) imaju veliku specifičnu površinu (oko 1000 m2 / g) koja se može koristiti za različite funkcionalizacije, punjenje/adsorpciju različitih molekula i njihovo uklanjanje, ili kao platforma za signalne molekule za detekciju različitih analita.

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Mezoporozni organosilikatni nanomaterijal (Periodični mezoporozni organosilikat (PMO)) sadrži veliki procenat organskih grupa, što omogućuje da ispoljavaju jedinstvene karakteristike za različite primene.

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Naše istraživanje se provodi u cilju dizajniranja novih serija MSN i PMO nanomaterijala, kao i njihovih jezgro / ljuska analoga.

Površina ovih nanomaterijala se modifikuje različitim molekulima kako bi specifično stupila u interakciju sa željenim tkivima, zagađivačima ili analitima, radi njihove efikasne primene u praćenju i zaštiti zdravlja, hrane i životne sredine.

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Jedan od ciljeva istraživanja je razvoj pametnih nanomaterijala sa stimulans-responzivnim oslobađanjem molekula iz MSN / PMO materijala, sa primenom u dijagnostici, snimanju i terapiji tkiva raka.
Kompozitni materijali polimer-MSN se takođe razvijaju u cilju primene kao antimikrobnih pametnih ambalaža za hranu i medicinskih implanata.

Konačno, nove PMO nanočestice se konstruišu radi efikasnog blokiranja UVA i UVB zračenja i primene u bezbednijoj generaciji krema za sunčanje.

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<img class="alignnone wp-image-1626 size-full" src="https://api.biosens.rs/wp-content/uploads/2021/06/slika-1.png" alt="" width="837" height="466" />

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Projekti:

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PRECAST: Prediction of Cancer Treatment Effectiveness with Stimuli-responsive Nanomaterials, Science Fund of the Republic of Serbia

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<a href="https://cordis.europa.eu/project/id/952259">NANOFACTS: Networking Activities for Nanotechnology-Facilitated Cancer Theranostics H2020-WIDESPREAD-2020-5</a>

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Novel Active Components for Health- and Enviroment-friendly UV-Blocking Based on Enriched Organosilica Nanomaterials, Project ID: 5566, The Innovation Fund of the Republic of Serbia

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III 44006 Razvoj novih informaciono-komunikacionih tehnologija, korišćenjem naprednih matematičkih metoda, sa primenama u medicini, telekomunikacijama, energetici, zaštiti nacionalne baštine i obrazovanju, Ministarstvo prosvete, nauke i tehnološkog razvoja Republike Srbije.

REALSENSE1 – Monitoring of cell culture parameters using sensors for biomass and nutrients/metabolites in media: Lab-on-a-Chip (LOC) approach, 2019-2021; Good Food Institute 2018 Competitive Grant Program

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CBS

CST