Center for Biosystems

Center for sensing technologies

Project aim: RealSense2 will use mathematical modeling coupled to experimental validation to develop and optimize sensors for biomass &amp; nutrients/metabolites in media and enable their integration into commercial bioreactors. This will provide better bioprocess control, in-line monitoring and when combined with filtering, can enable media recycling which may reduce overall costs of proliferation steps for cultivated meat production.
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About the project: The specific objectives of the projects are a) to identify the most efficient configuration for integrating sensors into the stirred tank bioreactor through coupled mathematical modeling, computational fluid dynamics (CFD) and experiment, b) to develop and fabricate integration-ready sensors of biomass/nutrient/metabolite sensors ready for integration (TRL5-6) into the bioreactor and to integrate the fabricated sensors into the bioreactor following design identified through the CFD models as the most efficient, and c) to test the bioreactors with integrated sensors using commercially-relevant cell types provided by the advisory members. Importantly, cells of different origin will be tested i.e. mammalian and invertebrate.

Senior research associate in the field of technical / technological sciences, telecommunications and Information Technologies

Vasa Radonić

Zoran Pavlović was born in 1986. in Aleksinac, Serbia. He obtained a Diploma in 2010., and an M.Sc. degree in 2011. in Chemical Engineering at the Faculty of Technology and Metallurgy, University of Belgrade. Master thesis about electrochemical catalysts and cyclone flow reactor fuel cell system development was done at the Max Planck Institute for Dynamics of Complex Technical Systems, Magdeburg, Germany. He obtained a Ph.D. degree in 2017. in Heterogeneous Electrochemical catalysis (Dr. Rer. Nat - Doctor of Natural Sciences), from the Max Planck Institute for Chemical Energy Conversion, Muelheim an der Ruhr, Germany, as well as from Fritz Haber Institute. Berlin, Germany. During his Ph.D. studies, he presented his work in various electrochemical conferences in Germany and at the Electrochemical society conferences in the USA (Orlando - Florida, and San Diego - California). Since March 2018. he has been working in Pharmaceutical company Phytonet AG, Zurich, Switzerland, where he is involved in starting up a production site of Pharmaceutical food supplement products in Novi Sad, Serbia. He works there as a Production Manager and Manager of Technical systems while doing Process development, Pharmaceutical Technology Transfer, Quality control, and Business development. At the same time since March 2018. he has been working as an Assistant Professor at the Faculty of Pharmacy, the University of Business Academy in Novi Sad, where he gives lectures on Industrial Pharmacy, Organic, Bioorganic, Stereo, and Steroid Chemistry, and supervise B.Sc., M.Sc., and Ph.D. students during their thesis development, and during their practical training in Phytonet AG. Since January 2021, Zoran has been working at the BioSense Institute as a Senior Researcher, joining on many ongoing European projects. His main research interest is the electrochemical catalysis in renewable energy storage, development of bio-electrochemical sensors and materials for applications in agriculture, medicine, pharmacy, and environmental protection.

Zoran Pavlović

Dr Radonic received a PhD in electronics in 2010 at University of Novi Sad. He authored and co-authored 2 book chapters, 30 journal papers, more than 60 conference papers, 10 technical solutions, and has more than 440 citations. Dr Radonić was the project leader in four Serbian national projects, one project financed by European Bank for Research and Development, one Bilateral project between Serbia and Slovenia, project financed by Good Food Institute, USA and WP leader in one FP7 project–InnoSense and one H2020 project–ANTARES. He participates in a number of research FP6, FP7, H2020, COST, EUREKA, IPA projects. He is a member of IEEE and MAGEO (Microwaves in Agriculture, Environment and Earth Observation) groups and was a member of Technical Program Committees of EUROCON (Computer as Tool, 2015) conference, Salamanca, Spain and Sensordevices conference 2019, Nice, France. Steering committee member of the BioSense Institute 2015-2020, and the vice president of Scientific council of the BioSense Institute 2015-2020. From 2022, he is the assistant director for science. Reviewer in different scientific journals: Scientific Reports, Lab-on-Chip, IEEE Sensors Journal, MDPI Sensors, MDPI Electronics, Sensors and Actuators B, IEEE Wireless and component letters, Electronic letters, etc. The main expertise of Dr Radonic is microfluidics, sensors development (microwave, optical, microfluidic sensors and biosensors), electronic, RF and microwave passive components (filters, coupler, diplexer, etc.), antennas and RFID systems, periodical and artificial materials, fabrication technologies, measurements and characterization.

Stefan Jarić is employed at the Center for Sensing Technologies as a Research Assistant and a final-year PhD student in materials physics at the Department of Physics, Faculty of Sciences at the University of Novi Sad. Stefan works on the development and research of new solutions in the field of biosensors based on graphene field effect transistors and specific synthetic receptors (aptamers) for the detection of various analytes in the area of environmental protection (toxins) and medicine (biomarkers). His expertise is developing in the direction of micro and nanofabrication of sensor platforms (photolithography, physical vapor deposition of materials), characterization of materials (Raman spectroscopy, atomic force microscopy) and electrical characteristics of biosensors.

Stefan Jarić

Dr. Nikola Knežević is a Full research professor at the Center for Sensor Technologies at the Biosense Institute. He received his doctorate in 2009 at Iowa State University, USA, in synthesizing and functionalizing porous silica nanoparticles for stimuli-responsive and targeted drug delivery. He gained further research experience as a postdoctoral fellow at the University of Houston (research in the field of multistep organic synthesis), Universidad Complutense de Madrid (nanomaterials science), Institute Charles Gerhardt Montpellier (nanomaterials science), FP7 - ERA ERA Chairs postdoctoral fellow at the Vinča Nuclear Institute and as Pole Chimie Ballard Visiting Professor at the University of Montpellier. Nikola is the author/co-author of over 40 scientific publications, including 2 book chapters and one patent, with over 1900 citations and an H index of 21. Nikola is the coordinator of one H2020 project (NANOFACTS GA 952259), three Serbian national projects (PRECAST – 6060755, proof of concept – 5566, technology transfer -1135), and two bilateral research projects (with France and Germany). Research interests: Synthesis of functional bioresponsive nanomaterials for use in health protection and construction of biosensors.

Nikola Knežević

Nejra Omerović is a Research Assistant at the BioSense Institute. She received her BSc and MSc degree in 2015 and 2017, respectively, from the Faculty of Technology, University of Novi Sad, Serbia, where she is currently a PhD student at the Department of Materials engineering. From October 2015 to September 2016 she did an internship at AGFA-Gevaert NV, Mortsel, Belgium, where she worked at the Inkjet Ink Development Department. Her current research activities are focused on biopolymers and their nanocomposites.

Nejra Omerović

Dr Marko Radović graduated in 2005 from the Faculty of Physics, University of Belgrade. In 2008 he received a MSc degree at the Faculty of Physics, University of Belgrade. He received PhD title in 2015 also at Faculty of Physics, University of Belgrade. From April 2006. to February of 2017. He was employed at the Institute of Physics in Belgrade. Since February 2017. He is employed as a senior researcher at BioSense Institute. Dr Radović research interests are focused on a bottom-up approach, involving synthesis, characterization and functionalization of nanomaterials to design of gas monitoring platforms, biosensors, optoelectronic and photocatalytic devices. He has published 25 scientific papers in international and domestic journals and he is a member of the Serbian Society for Ceramic Materials.

Marko Radović

Ivana Podunavac is a Junior Researcher at BioSense Institute and PhD student of Applied Physics at the Faculty of Sciences, University of Novi Sad, Serbia. Her fields of research are microfluidics,
simulations, fabrication and testing of sensing devices.

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Ivana Podunavac

Goran Kitić works at the position of the Head of the Center for Sensing Technologies within the BioSense Institute. He received a PhD degree from the Faculty of Technical Sciences, University of Novi Sad, Serbia in 2016 on the subject of microwave soil moisture sensors. Goran Kitić developed experience in the field of different fabrication technologies (LTCC, thin&amp;thick film technology, microfabrication, PCB, wire bonding, machining, and 3D printing) and characterization methods (electron microscopy, EDS spectroscopy, UV/Vis spectroscopy, optical profilometry, electrical measurements). His research interests are related to the development of sensors applied in agriculture, food sector and environmental protection. Some of the developed sensor prototypes involve a multispectral sensor for plant status assessment, soil moisture sensor, a device for early mastitis detection, nitrates in water detector. Besides stated, he is heavily involved in the development of a robotic system for soil sampling and analysis. His research work resulted in seven international journal publications, and numerous papers published on international conferences. During his research career, he was involved in 13 international and national projects.

Goran Kitić

Mila Djisalov has a BSc in Biochemistry and a Master’s degree in Molecular Biology. She is currently a Ph.D. Student of Biochemistry at the Faculty of Sciences in Novi Sad and a Junior Researcher in BioSense Institute. Her main research interests are biosensor development and molecular tools for pathogen detection, cellular agriculture, and the development of lab-on-a-chip platforms. Mila is also one of the co-founders of the student group The BioSense Alt. Protein Project formed at the BioSense Institute.

MIla Đisalov

Ivana Gadjanski PhD is Research Associate Professor at BioSense Institute, University of Novi Sad, Serbia. She graduated from the Faculty of Biology, University of Belgrade as the 1st in the class (2003). She obtained PhD in Neuroscience (summa cum laude -oral presentation) from Georg-August University &amp; Max Planck Institute for Biophysical Chemistry, Goettingen, Germany (2007) and worked as a postdoctorate researcher at Ludwig-Maximillian University, Munich, Germany. Ivana then turned to the bioengineering field and spent a year as a Fulbright Visiting Scholar at Columbia University, USA - Lab for Stem cells and Tissue Engineering (Head: prof. Gordana Vunjak-Novakovic). In BioSense, she is moving further into the cellular agriculture field - developing solutions for cultivated meat. She also works on biosensing for agrifood, biomedicine and environmental monitoring. Ivana is a grantee of the Good Food Institute and coordinator of Horizon2020 projects DRAGON GA 810755 and IPANEMA GA 872662.

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Ivana Gađanski

RealSense1 i2 (1) 1

THE GOOD FOOD INSTITUTE GRANTS

BioSense participates in a range of pan-European initiatives that aim to centralise environmental data coming from various regions, sources and institutions. Such a database would provide researchers, government institutions and NGOs precious information about the state of ecosystems around Europe along with an insight on how they are changing in time.

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Environmental and ecological data is very heterogeneous. It encompasses logs from sensors, expert observations, photos from smartphones and UAVs, lab analyses and others. Individual data from one source or one region provides little information, as it cannot be analysed in a wider context. Also, data is usually kept at individual institutions separately, which poses further hurdles for comprehensive analysis and directly contradicts the EU principles of open science.

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For these reasons, we participate in a suite of European projects where we are developing a platform for deposition of environmental and ecological data. The platforms facilitates networking between European institutions and a collaborative approach to tackling the problems of climate change, global warming, habitat loss and altered competitive relationships within flora and fauna.

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<img class="alignnone wp-image-1847 size-full" src="https://api.biosens.rs/wp-content/uploads/2021/07/GIS.png" alt="" width="940" height="475" />

<img class="alignnone wp-image-1848 size-full" src="https://api.biosens.rs/wp-content/uploads/2021/07/GIS-1.png" alt="" width="940" height="477" />

<img class="alignnone wp-image-1849 size-full" src="https://api.biosens.rs/wp-content/uploads/2021/07/GIS-2.png" alt="" width="850" height="530" />

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Ecology Platforms

Due to their small diameter (ca. 100 nm) and highly porous nature, mesoporous silica nanoparticles (MSNs) have high specific surface area (ca. 1000 m2/g) which can be utilized for a plethora of functionalizations, loading/adsorption of different molecules and their removal, or as a platform for signaling molecules to detect different analytes.

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Mesoporous organosilicate nanomaterial (also termed Periodic Mesoporous Organosilica (PMO)) contains a large percentage of organic groups within the nanosphere, exhibiting unique characteristics for different applications.

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Our research is being conducted toward design of new series of MSN and PMO nanomaterials, as well as their core/shell analogs.

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Surface of these nanomaterials is being modified with different molecules to specifically interact with desired tissues, pollutants, or analytes, for their efficient application in monitoring and protection of health, food and the environment.

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One of the research goals is to develop smart nanomaterials with stimuli-responsive release of MSN/PMO-loaded molecules for diagnostics, imaging, and therapy of cancer tissues.

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Polymer-MSN composite materials are also being developed for applications as antimicrobial smart food packaging and medical implants.

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Finally, PMO nanoparticles are being constructed for efficient UVA and UVB blocking and application in a safer generation of sunscreens.

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<img class="alignnone wp-image-1626 size-full" src="https://api.biosens.rs/wp-content/uploads/2021/06/slika-1.png" alt="" width="837" height="466" />

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<a href="https://api.biosens.rs/wp-content/uploads/2021/06/68.tif"><img class="alignnone size-medium wp-image-1526" src="https://api.biosens.rs/wp-content/uploads/2021/06/68.tif" alt="" width="1" height="1" /></a>

Projects:

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PRECAST: Prediction of Cancer Treatment Effectiveness with Stimuli-responsive Nanomaterials, Science Fund of the Republic of Serbia

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<a href="https://cordis.europa.eu/project/id/952259">NANOFACTS: Networking Activities for Nanotechnology-Facilitated Cancer Theranostics H2020-WIDESPREAD-2020-5 </a>

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Novel Active Components for Health- and Environment-friendly UV-Blocking Based on Enriched Organosilica Nanomaterials, Project ID: 5566, The Innovation Fund of the Republic of Serbia

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III 44006, Ministry of Education, Science, and Technological Development of the Republic of Serbia.

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Silica nanomaterials

Biosensors are analytical devices for detection of the biologic material which may include biomolecules, biological structures or microorganisms (bacteria, viruses, algae, spores). Nowadays, they are being used for food safety evaluation, environmental monitoring, or for early detection of infectious diseases in biomedicine.

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We work on the development of new point-of-need biosensor transducers, biofunctionalization of the sensors with bioreceptors, and sensor integration into microfluidic systems. Developed biosensors are being implemented for detection of bacteria in food and water, detection of cyanobacterial toxins produced during harmful algal blooms, as well as for a fast detection of faecal bacteria and other microbiological pathogens in water.

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More concrete, we focus our research on development of new nanomaterials for functionalization of the biosensor electrodes and fabrication of new electrochemical transducers using different fabrication technologies. Particular attention is dedicated to new materials which will enable selective and efficient binding of the analytes and to the novel biosensor designs to increase the selectivity, sensitivity and limit of detection. Therefore, we develop the novel methods of graphene modification, and the mask-less methods of graphene patterning and integration to conventional industrial routes.

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A special focus is placed on biosensors containing enzymes, antibodies and nucleic acids (DNA, RNA) as recognition elements. This research is performed in close collaboration with the Center for Biosystems in which experts work on biomolecular engineering and molecular detection methods including the cutting-edge isothermal methods for nucleic acid amplification such as LAMP (loop-mediated isothermal amplification) and RPA (recombinase polymerase amplification) and other ways of molecular detection which can be well combined with the biosensing methods.

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In addition, BioSense Institute works on the new types of biosensors for monitoring nutrients and products of cellular metabolism during cultured meat production, as another example of excellent multidisciplinary collaboration with the Center for BioSystems and the researchers working on Cellular agriculture topics.

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<img class="alignnone wp-image-1508 " src="https://api.biosens.rs/wp-content/uploads/2021/06/58.png" alt="" width="701" height="417" />

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Collaborative projects with the Center for Biosystems:

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<a href="http://www.ipanema2020.com/">H2020 MSCA-RISE IPANEMA GA 872662</a> - Integration of PAper-based Nucleic acid testing mEthods into Microfluidic devices for improved biosensing Applications

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<a href="http://www.realsense.rs/">REALSENSE1</a> - Monitoring of cell culture parameters using sensors for biomass and nutrients/metabolites in media: Lab-on-a-Chip (LOC) approach, 2019-2021; Good Food Institute Inaugural Competitive Grant Program.

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<a href="http://www.realsense.rs/">REALSENSE2</a> – From lab-on-a-chip to custom bioreactor: scale up modeling study, 2020-2022 Good Food Institute Competitive Grant Program

Biosensors

Cellular agriculture refers to production of agricultural products using cell cultures. Cellular agriculture products are part of the larger group called Alternative proteins (alt.pro). Alt.pro are proteins that can act as substitutes for traditional animal‐based food. Generally, alt.pro are classified based on the source into: Plant-based, Insect-based and Cultured meat that is produced by cellular agriculture methods. Majority of the methods for cellular agriculture stem from the tissue engineering and biomedical field, while the application of the products generated by cellular agriculture falls predominantly into the food science.

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Cultured meat is the main type of alternative proteins BioSense addresses. One of the main challenges for scaling up the cultured meat production to the industry levels is concerning the overall costs of production. The highest portion of costs (up to 80%) for cultured meat production is for the cell culture medium and fetal calf or bovine serum. The challenge to decrease expenses is not only in designing low-cost cell culture medium formulations, but also in developing methods to optimize the bioreactor design, instrumentation and to include monitoring features via sensors for the maximum cell production capacity per unit medium volume.

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In that sense, we focus our research on optimizing procedures for generating cellular products, and we closely collaborate with the Center for sensing technologies regarding development of sensors for monitoring of key parameters in cultured meat bioprocess: Biomass concentration, a key variable in cell cultures and Concentration of key nutrients – glucose and glutamine and their main metabolites, lactate and ammonia, that can inhibit the cell growth. Sensors are tested using "miniaturized" bioreactors i.e. microfluidic model and Lab-on-a-chip (LOC) principle, which will be scaled up to the bioreactors of larger volumes.

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Projects:

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REALSENSE1 - Monitoring of cell culture parameters using sensors for biomass and nutrients/metabolites in media: Lab-on-a-Chip (LOC) approach, 2019-2021; Good Food Institute Inaugural Competitive Grant Program.

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REALSENSE2 - From lab-on-a-chip to custom bioreactor: scale up modeling study, 2020-2022 Good Food Institute Competitive Grant Program

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<img class="alignnone wp-image-1459 size-large" src="https://api.biosens.rs/wp-content/uploads/2021/06/31-1024x644.png" alt="" width="1024" height="644" />

Cellular agriculture

REALSENSE2- FROM LAB-ON-A-CHIP TO CUSTOM BIOREACTOR: SCALE UP MODELING STUDY, 2019-2021; Good Food Institute 2018 Competitive Grant Program

Centar za Biosisteme

Centar za senzorske tehnologije

Cilj projekta: RealSense2 fokusiran na matematičko modelovanje povezano sa eksperimentalnom validacijom kako bi razvili i optimizovali novi senzori za biomasu i hranljive sastojke/metabolite u hranljivom medijumu čime bi se omogućila njihova integraciju u komercijalne bioreaktore. Ovo će obezbediti bolju kontrolu bioprocesa, redovno praćenje, a u kombinaciji sa filtriranjem, može omogućiti recikliranje medijuma što će rezultovati u smanjenju ukupnih troškova neophodnih za proizvodnju kultivisanog mesa.
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O projektu: Specifični ciljevi projekata su: a) identifikacija najefikasnije konfiguracije za integrisanje senzora u bioreaktor sa mešanim rezervoarom kroz povezano matematičko modelovanje, dinamiku fluida i eksperimente, b) razvoj senzora za hranljive sastojke/metabolite spremne za integraciju (TRL5-6) u bioreaktor i za integrisanje proizvedenih senzora u bioreaktor prema dizajnu identifikovanom kroz najefikasniji matematički model, kao i c) za ispitivanje bioreaktora sa integrisanim senzorima koristeći komercijalno relevantnih tipovi ćelija. Testiraće se ćelije različitog porekla, tj. sisara i beskičmenjaka.

Viši naučni saradnik u oblasti tehničko–tehnoloških nauka, telekomunikacija i informacionih tehnologija

Goran Kitić je zaposlen na poziciji Rukovodioca centra za senzorske tehnologije na Institutu BioSens. Doktorirao je na Fakultetu tehničkih nauka, na Univerzitetu u Novom Sadu 2016. godine na temu mikrotalasnih senzora vlažnosti zemljišta. Goran Kitić ima iskustva u oblastima raznih tehnologija fabrikacije (LTCC, debeloslojna i tankoslojna tehnologija, mikrofabrikacija, PCB, wire bonding, mašinska obrada i 3D štampa) i metoda karakterizacije (elektronska mikroskopija, EDS spektoroskopija, UV/Vis spektroskopija, optička profilometrija, električna merenja). Njegova istraživanja su u vezi sa razvojem senzora koji svoju primenu imaju u poljoprivredi, prehrambenom sektoru i zaštiti životne sredine. Neki od razvijenih prototipa senzora uključuju multispektralni senzor za utvrđivanje statusa biljaka, senzor vlažnosti zemljišta, uređaj za rano otkrivanje mastitisa, detektor nitrata u vodi. Pored navedenog, u velikoj meri je uključen u razvoj robotskog sistema za uzorkovanje i analizu zemljišta. Njegovo istraživanje je publikovano u 7 međunarodnih časopisa, i brojnim radovima objavljenim na međunarodnim konferencijama. Tokom svoje karijere, učestvovao je u 13 međunarodnih i nacionalnih projekata.

Zoran Pavlović rođen je 1986. godine u Aleksincu, Srbija. Diplomu je stekao 2010. godine, a Master diplomu 2011. godine iz hemijskog inženjerstva na Tehnološko-metalurškom fakultetu Univerziteta u Beogradu. Master teza o elektrohemijskim katalizatorima i razvoju sistema gorivih ćelija sa protočnim ciklonskim reaktorima urađena je na Max Planck Institutu za dinamiku složenih tehničkih sistema, Magdeburg, Nemačka. Doktorirao je 2017. godine na heterogenoj elektrohemijskoj katalizi (dr. Rer. Nat - doktor prirodnih nauka), na Max Planck Institutu za hemijsku konverziju energije, Muelheim an der Ruhr, Nemačka, kao i na Fritz Haber Institutu, Berlin, Nemačka. Tokom doktorskih studija, predstavljao je svoj naucni rad na raznim elektrohemijskim konferencijama u Nemačkoj, kao i na konferencijama Elektrohemijskog društva u SAD-u (Orlando - Florida i San Diego - Kalifornija). Od marta 2018. godine radi u farmaceutskoj kompaniji Phytonet AG, Zurich, Švajcarska, gde je uključen u pokretanje proizvodnog pogona farmaceutskih preparata dodataka ishrani u Novom Sadu. Tamo radi kao menadžer proizvodnje i menadžer tehničkih sistema, gde se bavi optimizacijom postojećih i razvojem novih farmaceutskih procesa proizvodnje i transfera tehnologija, kao i poslovnim razvojem,. U isto vreme, od marta 2018. godine radi kao docent na Farmaceutskom fakultetu Univerziteta u Novom Sadu, gde vodi predavanja iz Industrijske farmacije, Organske, Bioorganske, Stereo i Steroidne hemije, a takođe je i mentor studentima na master i doktorskim tezama, kao i na studentskim praksama u Phytonet-u. Od januara 2021. godine, Zoran radi na Institutu BioSense kao Stariji istraživač, pridružujući se brojnim tekućim evropskim projektima. Njegov glavni istraživački interes je elektrokemijska kataliza u cilju skladištenja energije dobijene obnovljivim izvorima energije, i razvoj bio-elektrohemijskih senzora i materijala za primenu u poljoprivredi, medicini, farmaciji i zaštiti životne sredine.

Dr Radonić je doktorirao iz oblasti elektronike 2010. godine na Univerzitetu u Novom Sadu. Autor je 2 poglavlja u knjizi, 30 radova u naučnim časopisima, više od 60 konferencijskih radova, 10 tehničkih rešenja i ima više od 440 citata. Dr Radonić je bio rukovodilac na četiri nacionalna projekta, jednom projektu koji je finansirala Evropska banka za istraživanje i razvoj, jednom bilateralnom projektu između Srbije i Slovenije, projektu koji je finansirao Good Food Institute iz SAD-a i vođa radnog paketa u jednom FP7 projektu – Innosens i jednom H2020 – Antares. Učestvovao je u brojnim istraživačkim projektima iz programa FP6, FP7, H2020, COST, Eureka, IPA. Član je grupa IEEE i MAGEO (Mikrotalasi u poljoprivredi, životnoj sredini i posmatranju Zemlje) i član tehničkih programskih odbora konferencije EUROCON (Computer as a Tool, 2015), Salamanka, Španija i Sensodevice konferencije 2019, Nica, Francuska. Član upravnog odbora Instituta BioSense od 2015-2020 i potpredsednik Naučnog veća Instituta BioSense 2015-2020. Od 2022 obavlja funkciju pomoćnika direktora za nauku. Recenzent u mnogim naučnim časopisima: Scientific Reports, Lab-on-Chip, IEEE Sensors Journal, MDPI Sensors, MDPI Electronics, Sensors and Actuators B, IEEE Wireless and component letters, Electronic letters, etc. Oblast naučnog istraživanja dr Radonića je mikrofluidika, razvoj senzora (mikrotalasni, optički, mikrofluidni senzori i biosenzori), elektronskih, RF i mikrotalasnih pasivnih komponenti (filteri, spojnica, diplekser itd.), antena i RFID sistema, periodični i veštački materijali, tehnologije fabrikacije, merenja i karakterizacija.

Stefan Jarić je zaposlen u Centru za senzorske tehnologije kao istraživač-saradnik i student završne godine doktorskih studija fizike materijala na Departmanu za fiziku, Prirodno-matematičkog fakulteta u Novom Sadu. Stefan se bavi razvojem i istraživanjem novih rešenja na polju biosenzora zasnovanih na grafenskim tranzistorima sa efektom polja i specifičnih sintetičkih receptora (aptamera) za detekciju raznih analita iz sfere zaštite životne sredine (toksini) i medicine (biomarkeri). Njegova ekpertiza se razvija u pravcu mikro i nanofabrikacije senzorskih platformi (fotolitografija, fizičko naparavanje materijala), karakterizacije materijala (Ramanova spektroskopija, mikroskopija atomskih sila) i električnih karakteristika biosenzora.

Dr Nikola Knežević je Naučni savetnik u Centru za senzorske tehnologije na Institutu Biosens. Doktorirao je 2009. godine na Iowa State University, SAD, iz oblasti sinteze i funkcionalizacije poroznih silikatnih nanočestica za stimulans-responsivnu i ciljajuću isporuku lekova. Dalje istraživačko iskustvo stekao je kao postdoktorand na Univerzitetu u Hjustonu (istraživanje iz oblasti višestepene organske sinteze), Universidad Complutense de Madrid (nauka o nanomaterijalima), Institute Charles Gerhardt Montpellier (nauka o nanomaterijalima), FP7 – ERA ERA Chairs postdoktorand u Nuklearnom institutu Vinča i kao Pole Chimie Balard gostujući profesor na Univerzitetu u Monpeljeu. Nikola je autor/koautor na preko 40 naučnih publikacija, uključujući 2 poglavlja u knjigama i jedan patent, sa preko 1900 citata i H indeksom 21. Nikola je koordinator jednog projekta H2020 (NANOFACTS GA 952259), tri srpska nacionalna projekta (PRECAST – 6060755, dokaz koncepta – 5566, transfer tehnologija -1135) i dva bilateralna istraživačka projekta (sa Francuskom i Nemačkom). Istraživačka interesovanja: sinteza funkcionalnih bioresponsivnih nanomaterijala za primenu u zaštiti zdravlja i konstrukciji biosenzora.

Nejra Omerović je zaposlena na Institutu BioSens kao istraživač saradnik. Osnovne i master studije završila je 2015., odnosno 2017. godine na Tehnološkom fakultetu, Univerzitet u Novom Sadu, Srbija, gde je trenutno sudent doktorskih studija na Katedri za Inženjerstvo materijala. Od oktobra 2015. do septembra 2016. godine obavljala je stručnu praksu u kompaniji AGFA-Gevaert NV, Mortsel, Belgija, gde je radila na Departmanu za razvoj inkdžet mastila. Njene trenutne istraživačke aktivnosti usmerene su na biopolimere i njihove nanokompozite.

Dr Marko Radović je diplomirao 2005. na Fizičkom Fakultetu pri Beogradskom univerzitetu. 2008. je odbranio magistarski rad na Fizičkom Fakultetu na Univerzitetu u Beogradu. Doktorsku disertaciju je odbranio 2015. na Fizičkom Fakultetu u Beogradu. Od aprila 2006. je zaposlen na Institutu za Fiziku u Beogradu. Od februara 2015. je zaposlen kao naučni saradnik na Biosens Institutu. Naučno istraživačka delatnost Dr Radovića je fokusirana na tzv. “bottom-up approach” gde su povezane sinteza nanomaterijala, karakterizacija I funkcionalizacija nanomaterijala u dizajnu senzorskih optoelektronskih i fotokatalitičkih uređaja. Tokom dosadašnje karijere publikovao je više od 25 naučnih radova u međunarodnim i domaćim časopisima.

Ivana Podunavac je istraživač pripravnik na Institutu BioSens i student doktorskih studija primenjene fizike na Prirodno-matematičkom fakultetu, Univerziteta u Novom Sadu. Njene oblasti istraživanja su mikrofluidika, kao i izrada i testiranje senzora.

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Mila Đisalov je diplomirani biohemičar i master molekularne biologije. Trenutno je student doktorskih studija biohemije na Prirodno-matematičkom fakultetu u Novom Sadu i istraživač-pripravnik na Institutu BioSens. Njena glavna istraživačka interesovanja vezana su za razvoj biosenzora i molekularnih alata za detekciju patogena, ćelijska poljoprivreda i razvoj laboratorija na čipu. Mila je takođe jedan od osnivača studentske grupe The BioSense Alt. Protein Project, osnovane na Institutu BioSens.

Mila Đisalov

Dr Ivana Gađanski je viši naučni saradnik na Institutu Biosens, Univerzitet u Novom Sadu, Srbija. Diplomirala je na Biološkom fakultetu Univerziteta u Beogradu kao prva u klasi (2003). Doktorirala je iz neuronauka (ocena summa cum laude – usmena odbrana) na Georg-August Univerzitetu i Institutu za biofizičku hemiju Maks Plank, Getingen, Nemačka (2007) i radila na posledoktorskim studijama na Univerzitetu Ludvig-Maksimilijian, Minhen, Nemačka. Ivana se zatim posvetila oblasti bioinženjerstva i provela godinu dana kao Fulbrajt stipendista – gostujući istraživač na Univerzitetu Kolumbija, Njujork, SAD u Laboratoriji za matične ćelije i tkivno inženjerstvo (rukovodilac: prof. Gordana Vunjak-Novaković). U BioSensu radi na bioinženjerstvu u oblasti ćelijske poljoprivrede - razvija rešenja za kultivisano meso. Takođe radi na razvoju biosenzora za monitoring u oblasti poljoprivrede, biomedicine i zaštite životne sredine. Ivana je stipendista Instituta za dobru hranu i koordinator Horizont2020 projekata DRAGON GA 810755 i IPANEMA GA 872662.

GRANTOVI INSTITUTA DOBRE HRANE

Ćelijska poljoprivreda se bavi proizvodnjom poljoprivrednih proizvoda pomoću kultura ćelija i ulazi u oblast tzv. alternativnih proteina (alt.pro). Alt. pro su proteini koji se mogu koristiti kao zamena za uobičajene životinjske proteine. Alt.pro mogu biti biljnog porekla, mogu biti i od insekata, a posebna grupa proteina označena kao kultivisano meso se dobija pomoću metoda ćelijske poljoprivrede. Većina metoda ćelijske poljoprivrede potiče iz biomedicine i tkivnog inženjerstva, dok primene i upotreba proizvoda dobijenih ćelijskom poljoprivredom pretežno pripadaju oblasti tehnologije hrane.

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Kultivisano meso je osnovni tip alternativnih proteina kojim se bavi Institut BioSens. Jedan od glavnih izazova za skaliranje tj. industrijsku proizvodnju kultivisanog mesa su ukupni troškovi proizvodnje. Najveći deo troškova (čak do 80%) za kultivaciju mesa potiče od cene hranljivog medijuma i fetalnog goveđeg seruma, neophodnih za gajenje ćelija u kulturi. Troškovi se mogu smanjiti ne samo jeftinijim formulacijama hranljivog seruma, već i putem metoda optimizacije dizajna bioreaktora i instrumenata i naročito upotrebom senzorskog sistema praćenja ključnih parametara, da bi se postigla maksimalna produkcija ćelija po jedinici zapremine medijuma.

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U tom smislu se fokusiramo na optimizaciju procedura za dobijanje ćelijskih produkata, a u bliskoj saradnji sa Centrom za senzorske tehnologije razvijamo i senzore za praćenje ključnih parametara u bioprocesu kultivacije mesa: koncentracija biomase, što je osnovni parametar ćelijske kulture i koncentracija osnovnih hranljivih materija – glukoze i glutamina i njihovih glavnih metabolita, laktata i amonijaka, koji mogu inhibirati rast ćelija. Senzori se testiraju na „minijaturizovanim“ bioreaktorima tj. mikrofluidnim modelima uz primenu „Laboratorija na čipu“ principa, koji će se zatim skalirati na bioreaktore većih zapremina.

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Projekti:

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REALSENSE1 - Monitoring of cell culture parameters using sensors for biomass and nutrients/metabolites in media: Lab-on-a-Chip (LOC) approach, 2019-2021; Good Food Institute Inaugural Competitive Grant Program.

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REALSENSE2 - From lab-on-a-chip to custom bioreactor: scale up modeling study, 2020-2022 Good Food Institute Competitive Grant Program

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<img class="alignnone wp-image-1459 size-large" src="https://api.biosens.rs/wp-content/uploads/2021/06/31-1024x644.png" alt="" width="1024" height="644" />

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Ćelijska poljoprivreda

Biosenzori su analitički uređaji koji služe za detekciju biološkog materijala kao što su biomolekuli, biološke strukture ili mikroorganizmi (bakterije, virusi, alge, spore). Široku primenu biosenzori nalaze u kontroli bezbednosti hrane, monitoringu životne sredine, kao i u ranom otkrivanju infektivnih bolesti u biomedicini.

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Naša istraživanja su usmerena na razvoj novih biosenzorskih transdjusera, biofunkcionalizaciju senzora sa bioreceptorima, i integraciju senzora u mikrofluidične sisteme. Razvijeni senzori se koriste za detekciju bakterija u hrani i vodi, za detekciju cijano toksina nastalih tokom cvetanja algi, kao i za brzu detekciju fekalnih bakterija i drugih mikrobioloških patogena u vodi.

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Bavimo se i razvojem novih nanomaterijala za funkcionalizaciju biosenzorskih elektroda i fabrikaciju novih elektrohemijskih biosenzora koristeći različite tehnologije fabrikacije. Deo istraživanja je usmeren na nove materijale koji će omogućiti selektivno i efikasno vezivanje analita, kao i na projektovanje biosenzora u cilju poboljšanja njihove selektivnosti, osetljivosti i limita detekcije. Bavimo se i novim metodama modifikacije grafena, novim metodama za njegovu sintezu i integraciju u konvencionalne industrijske procese.

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Posebna pažnja je posvećena biosenzorima na bazi enzima, antitela i nukleinskih kiselina (DNK, RNK). Ova istraživanja se izvode u bliskoj saradnji sa Centrom za biosisteme u kojem istraživači rade na metodama biomolekularnog inženjerstva i molekularne dijagnostike što uključuje i najsavremenije izotermalne metode za umnožavanje nukleinskih kiselina kao što su LAMP (Loop Mediated Isothermal Amplification) i RPA (Recombinase Polymerase Amplification) koje se mogu dobro kombinovati sa biosenzorima razvijenim u okviru Centra za senzorske tehnologije.

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Takođe, Institut BioSens radi na novim vrstama biosenzora za monitoring hranljivih materija i produkata metabolizma ćelija u procesu proizvodnje kultivisanog mesa, što je još jedan primer dobre multidisciplinarne saradnje sa Centrom za biosisteme i istraživačima koji rade u okviru oblasti ćelijske poljoprivrede.

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<img class="alignnone wp-image-1508 " src="https://api.biosens.rs/wp-content/uploads/2021/06/58.png" alt="" width="701" height="417" />

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Zajednički projekti sa Centrom za biosisteme:

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H2020 MSCA-RISE IPANEMA GA 872662 - Integration of PAper-based Nucleic acid testing mEthods into Microfluidic devices for improved biosensing Applications

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REALSENSE1 - Monitoring of cell culture parameters using sensors for biomass and nutrients/metabolites in media: Lab-on-a-Chip (LOC) approach, 2019-2021; Good Food Institute Inaugural Competitive Grant Program.

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REALSENSE2 – From lab-on-a-chip to custom bioreactor: scale up modeling study, 2020-2022 Good Food Institute Competitive Grant Program

Biosenzori

BioSens učestvuje u raznim panevropskim inicijativama kojima je cilj da centralizuju podatke o životnoj sredini koji dolaze iz različitih regiona, izvora i institucija. Takva baza podataka pružila bi istraživačima, vladinim institucijama i nevladinim organizacijama dragocene informacije o stanju ekosistema širom Evrope, kao i uvid u to kako se oni vremenom menjaju.

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Ekološki podaci su vrlo heterogeni. Obuhvataju zapise sa senzora, stručna zapažanja, fotografije sa pametnih telefona i dronova, laboratorijske analize i druge. Pojedinačni podaci iz jednog izvora ili jednog regiona pružaju malo informacija, jer se ne mogu analizirati u širem kontekstu. Takođe, podaci se obično čuvaju u pojedinačnim institucijama odvojeno, što predstavlja dodatne prepreke za sveobuhvatnu analizu i direktno je u suprotnosti sa principima otvorene nauke EU.

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Iz tih razloga učestvujemo u nizu evropskih projekata gde razvijamo platformu za čuvanje podataka o životnoj sredini i ekologiji. Platforme olakšavaju umrežavanje između evropskih institucija i zajednički pristup rešavanju problema klimatskih promena, globalnog zagrevanja, gubitka staništa i izmenjenih odnosa unutar flore i faune.

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<img class="alignnone wp-image-1847 size-full" src="https://api.biosens.rs/wp-content/uploads/2021/07/GIS.png" alt="" width="940" height="475" />

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<img class="alignnone wp-image-1848 size-full" src="https://api.biosens.rs/wp-content/uploads/2021/07/GIS-1.png" alt="" width="940" height="477" />

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<img class="alignnone wp-image-1849 size-full" src="https://api.biosens.rs/wp-content/uploads/2021/07/GIS-2.png" alt="" width="850" height="530" />

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Ekološke platforme

Zbog malog prečnika (oko 100 nm) i veoma porozne prirode, mezoporozne silikatne nanočestice (MSN) imaju veliku specifičnu površinu (oko 1000 m2 / g) koja se može koristiti za različite funkcionalizacije, punjenje/adsorpciju različitih molekula i njihovo uklanjanje, ili kao platforma za signalne molekule za detekciju različitih analita.

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Mezoporozni organosilikatni nanomaterijal (Periodični mezoporozni organosilikat (PMO)) sadrži veliki procenat organskih grupa, što omogućuje da ispoljavaju jedinstvene karakteristike za različite primene.

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Naše istraživanje se provodi u cilju dizajniranja novih serija MSN i PMO nanomaterijala, kao i njihovih jezgro / ljuska analoga.

Površina ovih nanomaterijala se modifikuje različitim molekulima kako bi specifično stupila u interakciju sa željenim tkivima, zagađivačima ili analitima, radi njihove efikasne primene u praćenju i zaštiti zdravlja, hrane i životne sredine.

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Jedan od ciljeva istraživanja je razvoj pametnih nanomaterijala sa stimulans-responzivnim oslobađanjem molekula iz MSN / PMO materijala, sa primenom u dijagnostici, snimanju i terapiji tkiva raka.
Kompozitni materijali polimer-MSN se takođe razvijaju u cilju primene kao antimikrobnih pametnih ambalaža za hranu i medicinskih implanata.

Konačno, nove PMO nanočestice se konstruišu radi efikasnog blokiranja UVA i UVB zračenja i primene u bezbednijoj generaciji krema za sunčanje.

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<img class="alignnone wp-image-1626 size-full" src="https://api.biosens.rs/wp-content/uploads/2021/06/slika-1.png" alt="" width="837" height="466" />

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Projekti:

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PRECAST: Prediction of Cancer Treatment Effectiveness with Stimuli-responsive Nanomaterials, Science Fund of the Republic of Serbia

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<a href="https://cordis.europa.eu/project/id/952259">NANOFACTS: Networking Activities for Nanotechnology-Facilitated Cancer Theranostics H2020-WIDESPREAD-2020-5</a>

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Novel Active Components for Health- and Enviroment-friendly UV-Blocking Based on Enriched Organosilica Nanomaterials, Project ID: 5566, The Innovation Fund of the Republic of Serbia

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III 44006 Razvoj novih informaciono-komunikacionih tehnologija, korišćenjem naprednih matematičkih metoda, sa primenama u medicini, telekomunikacijama, energetici, zaštiti nacionalne baštine i obrazovanju, Ministarstvo prosvete, nauke i tehnološkog razvoja Republike Srbije.

REALSENSE2- FROM LAB-ON-A-CHIP TO CUSTOM BIOREACTOR: SCALE UP MODELING STUDY, 2019-2021; Good Food Institute 2018 Competitive Grant Program

Teme:

Ljudi:

Centri:

CBS

CST