Center for Biosystems

Project aim: Improving the public health system to fulfill its role in the prevention and treatment of seasonal allergies
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About the project: Exposure to aeroallergens is associated with allergic rhinitis and asthma that significantly reduce quality of life and have a notable economic impact on society. Timely information on exposure risk is prerequisite for preventing symptoms and management allergic disease. There is a long-term awareness on the importance of aerobiological data in Europe, but applied methodology using the volumetric spore trap of the Hirst design provides only past data (1-7 days old). Such data, from public health point of view, can be used for observational retrospective analysis and strategy development only. In order to be of use for improvement the quality of life in pollen sensitive population, public health system in the region should provide real-time measurements of atmospheric pollen concentrations and forecasts, similarly as seen in the case of meteorology and air quality where assimilation of real-time data is necessary to increase the performance of atmospheric models.
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RealForAll modernized public health service in the region and notably enhanced the quality and applicative value of the information it provides by introducing real-time monitoring of airborne allergens, developing models for prediction exposure and by creating a joint platform for instantaneous dissemination of these information. In addition, the project made an effort to educate end-users on the benefits from using aerobiological information for prevention and management of allergy symptoms.
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Principal Research Fellow 

Branko Šikoparija

Predrag Radišić, born in 1962, has PhD in Biology and works as senior researcher at BioSense Institute and Chief at Laboratory for palynology - UNSPMF (ISO17025). He is a practiced researcher with a strong background in environmental science, ecology, aerobiology, allergy &amp; health, modelling &amp; forecasting.Predrag Radišić is a writer for multiple scientific research journals. He co-authored a monograph on fauna Syrphidae and allergenic pollen and published 36 scientific papers and more than 70 other scientific publications (GoogleSchoolarCitations H-index=8). As researcher, Predrag Radišić developed skills for applying for funding and he also acted as the investigator on a number national and international research projects (Ministry of Science-Serbia, SNSF-SCOPES, IPA, WUS-CEP&amp;CDP+). He participated in the registration of Protected Designations of Origin for Fruska Gora Lime Tree Honey and in two COST Actions ES0603 and FA1203.

Predrag Radišić

Dr Branko Sikoparija is a Principal Research Fellow at the Center for Biosystems and quality manager (ISO17025) in the Laboratory for Palynology University of Novi Sad Faculty of Sciences. His research focus is aerobiology and currently, he is interested in developing real-time detection and identification of bioaerosols. Branko Sikoparija is an accomplished writer and reviewer for multiple scientific research journals. Serves as Associate Editor for Aerobiologia. He coordinates the RealForAll network of automated bioaerosol monitors on the Pannonian plain. He is also an active member of the EUMETNET AutoPollen program, vice chair of the COST Action CA18226, the chair of the European Aerobiology Society Working Group for Quality Control, and principal investigator in the BREATHE project financed in the PROMIS call Science Fund of the Republic of Serbia.

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We study the diversity of bioaerosols so as the processes involved in the emission and dispersion of particles of biological origin in the atmosphere. By focusing on airborne pollen and fungal spores, we set the foundation for modelling of aerobiological phenomena and forecasting their impact.

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The group started to study pollen in 1989 focusing on feeding habits of insects, in particularly honeybees and hoverflies.  We supported the local Association of beekeepers “Dr Jovan Živanovic” to register the protected designation of origin for “Fruška gora lime tree honey”. Since 1999 the research was oriented towards the agents of allergies when the group started continuous sampling bioaerosols by using Hirst type volumetric pollen and spore traps.

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In the BioSense Institute, classical aerobiology has the chance to utilise advanced IT tools both for sampling and detection of bioaerosols and for data analytics. We are striving to set the basis for source-oriented modelling of airborne pollen concentrations. Therefore, we are coupling remote sensing data and airborne pollen data to map sources of the most important allergenic pollen, and setting field experiments to parametrize emission of the pollen.

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Our group is also exploring the possibilities for using flow cytometry in automated detection and quantification of bioaerosols (i.e. pollen and fungal spores). We are also developing an automated pollen monitoring system and compare its performance with classical Hirst trap and microscopy-based method.

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We host an hourly resolved long term collection of air samples from typical agriculture region. In addition, we have reference signals of scattering, fluorescence spectrum and fluorescence lifetime collected by RAPID-e device for the majority of airborne pollen.

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Projects:

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2017-2020 “Real-time measurements and forecasting for successful prevention and management of seasonal allergies in Croatia-Serbia cross-border region” – <a href="http://www.realforall.com">RealForAll</a>, INTERREG IPA-CBC Croatia Serbia

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2020-2022 “Real-time detection and quantification of bioaerosols relevant for human and plant health” – <a href="https://www.realforall.com/language/en/project-breathe/">BREATHE</a>, Fond za nauku Republike Srbije

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2016-2020 Monitoring polena u vazduhu na teritoriji AP Vojvodine, Pokrajinski sekretarijat za zaštitu životne sredine

Aerobiology and palynology

RealForAll -Real-time measurements and forecasting for successful prevention and management of seasonal allergies in Croatia-Serbia cross-border region – RealForAll (2017HR-RS151)

Centar za Biosisteme

Cilj projekta: Unapređivanje sistema javnog zdravstva kako bi ispunio svoju ulogu u prevenciji i lečenju sezonskih alergija.
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O projektu: Izloženost aeroalergenima je u u direktnoj vezi sa alergijskim rinitisom i astmom koji nepovoljno utiču na kvalitet života i mogu imati značajan negativan ekonomski uticaj. Odgovarajuća informacija o riziku za izloženost alergenima je preduslov za prevenciju simptoma alergije. Iako u Evropi, dugi niz godina postoji svest o važnosti aerobioloških podataka, primenjena metodologija omogućava njihovu dostupnost tek sa 1-7 dana zakašnjenja. Sa aspekta javnog zdravlja ovakvi podaci pomažu retrospektivnu analizu i stretegijsko planiranje, ali da bi omogućili unapređenje života za krajnje korisnike, moraju biti dostupni u realnom vremenu uz odgovarajuću prognozu (kao što je slučaj u meteorologiji).
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RealForAll omogućio je praćenje aeroalergena u realnom vremenu, razvio prognostičke modele i kreirao zajedničku platformu za diseminaciju merenih i prognoziranih podataka. Takođe, kroz projekat, vršene su edukacije korisnika o načinu upotrebe dostupnih aerobioloških podataka u prevenciji i svakodnevnom životu sa sezonskim alergijama.
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Naučni savetnik

Predrag Radišić, rođen 1962. godine, doktorirao je biologiju i radi kao viši istraživač na BioSense institutu i kao Šef u laboratoriji za palinologiju - UNSPMF (ISO17025). Sa višegodišnjim iskustvom se bavi istraživanjem u oblastima: zaštite životne sredine, ekologije, aerobiologije, alergijama i zdravlju, modeliranju i prognoziranju, Predrag Radišić je objavljivao radove u više naučno-istraživačkih časopisa. Koautor je monografija o fauni cvetnih muva (Diptera: Syirphidae), monografije o alergenom polenu i objavio 36 naučnih radova i više od 70 drugih naučnih publikacija (Google-SchoolarCitations H-indek = 8). Kao istraživač, Predrag Radišić je razvio veštine za prijavljivanje za finansiranje, a takođe je radio i kao istraživač na brojnim nacionalnim i međunarodnim istraživačkim projektima (Ministarstvo nauke-Srbija, SNSF-SCOPES, IPA, VUS-CEP &amp; CDP +). Učestvovao je u registraciji zaštićenih oznaka porekla za fruškogorski med od lipe i u dve COST akcije ES0603 i FA1203.

Dr Branko Šikoparijaje naučni savetnik u Centru za biosisteme i rukovodilac kvaliteta (ISO17025) u Laboratoriji za palinologiju na Prirodno-matematičkom fakultetu Univerziteta u Novom Sadu. Fokus istraživanja je aerobiolgija, a trenutno je zainteresovan za razvoj detekcije i identifikacije bioaerosola u realnom vremenu. Branko Šikoparija je publikovao i recenzirao naučne radove u brojnim naučnim časopisima. Radi i kao pomoćnik urednika časopisa Aerobiologia. Koordinator je RealForAll mreže za autmatski monitoring bioaerosola u Panonskoj niziji. Takođe, aktivan je član EUMETNET AutoPollen programa, zamenik predsedavajućeg COST Action CA18226, i predsedavajući radne grupe Evropskog Aerobiološkog Udruženja za kontrolu kvaliteta. Vodeći je istraživač BREATHE projekta koji u okviru PROMIS programa finansira Fond za nauku Republike Srbije.

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Proučavamo raznovrsnost bioaerosola i procese koji su uključeni u emisiju i disperziju čestica biološkog porekla u atmosferi. Razvijamo sinergiju između merenja bioaerosola u vazduhu i modeliranja njihovog atmosferskog ponašanja. Fokusirajući se na polen i spore gljiva postavili smo temelje za modeliranje aerobioloških pojava i predviđanje njihovog uticaja. Naša istraživanja vode ka razvoju atmosferske dijagnostike a s ciljem pružanja informacija o riziku za zdravlje kako ljudi tako i biljaka i životinja.
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Grupa je počela proučavati polen 1989. godine s naglaskom na prehrambene navike insekata, pogotovo pčela i muva lebdelica. Ovom ekspertizom u saradnji sa društvom pčelara “Dr Jovan Živanovič”, omogućili smo registraciju imena porekla za „Fruškogorski lipov med“. Od 1999. godine istraživanje je usmereno na uzročnike alergija, kada je grupa započela kontinuirano uzorkovanje bioaerosola primenom Hirstovih volumetrijskih klopki za polen i spore.
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U Institutu BioSens, imamo priliku da iskoristimo napredne IT alate kako za uzorkovanje i otkrivanje bioaerosola, tako i za analizu podataka. Nastojimo postaviti temelje za modeliranje atmosferskih koncentracija polena. Stoga povezujemo aerobiološke i satelitske podatke kako bismo mapirali izvore najvažnijeg alergenog polena. Postavljamo terenske eksperimente kako bismo parametrirali emisiju polena.
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Naša grupa takođe istražuje mogućnosti korišćenja protočne citometrije za automstsku detekciju i kvantifikaciju bioaerosola. Trenutno, razvijamo sistem automatskog prećenja polena suspendovanog u vazduhu i poredimo njegove performanse sa klasičnom Hirstovom volumetrijskom metodom i svetlosnom mikroskopijom.
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Posedujemo višegodišnju zbirku uzoraka vazduha iz tipične poljoprivredne oblasti koja je osnov za proučavanje raznovrsnosti bioaerosola i sintetisanje znanja o procesima povezanim sa kretanjem čestica biološkog porekla u atmosferi iznad tipičnih poljoprivrednih i urbanih područja. Takođe imamo referentne signale rasipanja, spektra fluorescence i vremena trajanja fluorescence.
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Projekti:

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2017-2020 “Real-time measurements and forecasting for successful prevention and management of seasonal allergies in Croatia-Serbia cross-border region” –<a href="http://www.realforall.com"> RealForAll</a>, INTERREG IPA-CBC Croatia Serbia

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2020-2022 “Real-time detection and quantification of bioaerosols relevant for human and plant health” –<a href="https://www.realforall.com/language/en/project-breathe/"> BREATHE</a>, Fond za nauku Republike Srbije

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2016-2020 Monitoring polena u vazduhu na teritoriji AP Vojvodine, Pokrajinski sekretarijat za zaštitu životne sredine

Aerobiologija i palinologija

RealForAll -Merenja u realnom vremenu i prognoza za uspešnu prevenciju i život sa sezonskim alergijama u prekograničnom području Hrvatska-Srbija- RealForAll (2017HR-RS151)

RealForAll -Merenja u realnom vremenu i prognoza za uspešnu prevenciju i život sa sezonskim alergijama u prekograničnom području Hrvatska-Srbija- RealForAll (2017HR-RS151)

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