Center for sensing technologies

Project aim: CISTEM aims to combine top notch microfluidic technologies and organ-on-a-chip concept, along with the technology of induced pluripotent stem cells, to design a new generation of heart-on-a-chip for the patient treatment.
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About the project: Many rare diseases cause chronic health problems or are even life-threatening. The impact on the quality of life of affected patients, of whom many are children, is significant. To date, a limited but increasing number of so-called orphan drugs (drugs for rare diseases) are reaching patients. However, most rare diseases are still without any effective treatment. The development of novel human systems for drug discovery therefore represents a major public health priority. Microfluidic technology-based “Organs-on-a-chip” represents a powerful tool for investigating rare disease mechanisms and testing new drug and treatment due to their ability to mimic tailored micro-environment architecture inspired by organ-level functions in vivo. However, due to the variability in rare disease mechanisms from one patient to another, organ on chip technology needs to be more precise and to convey towards personalized medicine. The human-induced pluripotent stem cells (iPSCs) have a strong potential in engineering organ-on-a-chip since they are derived in a patient-matched manner which makes them a superb source to construct human models for personalized drug screening as well as for understanding patient-specific fundamentals of diseases. To provide novel miniaturized platform for investigation of rare diseases able to address the burning issues in precision medicine today, CISTEM is bringing together and synergistically several highly promising research directions which are too often investigated separately at the academic level but also at the industrial level. CISTEM project is implemented by exploiting the complementary expertise of its partners and creating synergies between them through the targeted secondments of staff.
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<img decoding="async" loading="lazy" class="alignnone wp-image-693 size-full" src="https://api.biosens.rs/wp-content/uploads/2021/06/CISTEM-Photo.png" alt="" width="924" height="587" srcset="https://api.biosens.rs/wp-content/uploads/2021/06/CISTEM-Photo.png 924w, https://api.biosens.rs/wp-content/uploads/2021/06/CISTEM-Photo-300x191.png 300w, https://api.biosens.rs/wp-content/uploads/2021/06/CISTEM-Photo-768x488.png 768w" sizes="(max-width: 924px) 100vw, 924px" /><img decoding="async" loading="lazy" class="alignnone wp-image-695 size-full" src="https://api.biosens.rs/wp-content/uploads/2021/06/CISTEM-Photo.jpg" alt="" width="845" height="321" srcset="https://api.biosens.rs/wp-content/uploads/2021/06/CISTEM-Photo.jpg 845w, https://api.biosens.rs/wp-content/uploads/2021/06/CISTEM-Photo-300x114.jpg 300w, https://api.biosens.rs/wp-content/uploads/2021/06/CISTEM-Photo-768x292.jpg 768w" sizes="(max-width: 845px) 100vw, 845px" />

Research associate in the field of technical - technological and biotechnical sciences

Georges Dubourg

Dr Radonic received a PhD in electronics in 2010 at University of Novi Sad. He authored and co-authored 2 book chapters, 30 journal papers, more than 60 conference papers, 10 technical solutions, and has more than 440 citations. Dr Radonić was the project leader in four Serbian national projects, one project financed by European Bank for Research and Development, one Bilateral project between Serbia and Slovenia, project financed by Good Food Institute, USA and WP leader in one FP7 project–InnoSense and one H2020 project–ANTARES. He participates in a number of research FP6, FP7, H2020, COST, EUREKA, IPA projects. He is a member of IEEE and MAGEO (Microwaves in Agriculture, Environment and Earth Observation) groups and was a member of Technical Program Committees of EUROCON (Computer as Tool, 2015) conference, Salamanca, Spain and Sensordevices conference 2019, Nice, France. Steering committee member of the BioSense Institute 2015-2020, and the vice president of Scientific council of the BioSense Institute 2015-2020. From 2022, he is the assistant director for science. Reviewer in different scientific journals: Scientific Reports, Lab-on-Chip, IEEE Sensors Journal, MDPI Sensors, MDPI Electronics, Sensors and Actuators B, IEEE Wireless and component letters, Electronic letters, etc. The main expertise of Dr Radonic is microfluidics, sensors development (microwave, optical, microfluidic sensors and biosensors), electronic, RF and microwave passive components (filters, coupler, diplexer, etc.), antennas and RFID systems, periodical and artificial materials, fabrication technologies, measurements and characterization.

Vasa Radonić

Jovana Stanojev is a research assistant and a PhD student at the Faculty of Technology, University of Novi Sad. She studied materials engineering. She is working towards finishing her PhD thesis. Topic of her PhD thesis is "Development of uncooled infrared detector based on lead selenide". Her current research focuses are development of new materials for sensors, nanomaterials and nanomembranes, microfluidics, development of organ-on-a-chip platforms, cell culturing.

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Jovana Stanojev

In 2009, Georges Dubourg received the engineer degree in electronics from the University of Sciences and Technologies in Bordeaux, France. Afterwards, he received his Ph.D degree in microelectronic from the University of Bordeaux in 2012. During his Ph.D thesis, he introduced a new activity in the IMS laboratory concerning organic MEMS for biosensing applications. From 2013 to 2014, he worked in the flied of microfluidic as a postdoctoral research fellow in the Institute of Electronics, Microelectronics and Nanotechnology (IEMN) at the University of Lille, France. Then he undertook postdoctoral research in Serbia at the University of Novi Sad before taking up his current senior researcher position at BioSense Institute in which he has launched from scratch a new research activity in the field of flexible electronic and sensors. His research interests include the design, fabrication and characterization of flexible sensors and microfluidic devices for biomedical application. From 2018, Georges is project coordinator of CISTEM project (H2020‐MSCA‐RISE‐2017) which aims to develop a new generation of organ-on-chip for modeling cardiovascular diseases.

CISTEM Logo

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This project has received funding from the European Union’s Horizon 2020 research and innovation program under grant agreement No.778354.



Horizon 2020

Heart-on-chip based on induced pluripotent stem cell technology for patient treatment

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Portable mass spectrometer based on a quadrupole analyser for investigaiton of consumer food intake effects for personalised nutrition using.

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Skin protection from UV irradiation based on nanoparticles

Selective cancer therapy based on slow release of drugs from silica nanomaterials

Medical and healthcare applications

Microfluidics deals with manipulation and control of small quantity of fluids in micro- and nano-channels and as such, it initiated revolution in different fields including microbiology, medicine, and chemical analysis.

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We focus our research on analysis and design of different microfluidic components, development of the new materials for microfluidic chips, and integration of the microfluidics with different detection systems and electronics. To that end, we employ different fabrication technologies PDMS, LTCC, 3D and inkjet printing, bio-printing, xurography, and hybrid technologies.

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Since the utilization of only one microfabrication technology cannot fulfill all the requirements set by the microfluidic chip, we also employ hybrid fabrication approach, i.e. we combine various technologies with various materials to realize microfluidic chips with high resolution, small dimension, and high flow rate. For instance, hybrid approach is used to develop multilayered filtration units, high flow rate mixers, as well as pollen separation pinch flow.

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Also, we integrate microfluidic concept and reagent storages with various transduction principles and sensors, to develop lab-on-a-chip platform which finds application in in-field environment monitoring, biomedicine and food analysis.

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Moreover, we work on development of microfluidic reactor that integrates cell culture system, different sensors for nutrient concentration and metabolic waste products measurement, optical detection system and electronics into single chip.

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Our efforts are also focused on medical applications - we combine the top notch microfluidic technologies and organ-on-a-chip concept, along with the technology of induced pluripotent stem cells, to design a new generation of heart-on-a-chip for the patient treatment.
In addition, we develop a microfluidic platform able to reproduce the dynamic and heterogeneity of the soil conditions while implementing monitoring systems for investigating the root/plant–microbiome interactions in a microfluidic device.

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PROJECTS:

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CISTEM - Heart On chip based on induced pluripotent Stem cell Technology for personalized Medicine, H2020-MSCA-RISE-2017, GA No. 778354

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REALSENSE1 - Monitoring of cell culture parameters using sensors for biomass and nutrients/metabolites in media: Lab-on-a-Chip (LOC) approach, 2019-2021; Good Food Institute Inaugural Competitive Grant Program.

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IPANEMA - Integration of PAper-based Nucleic acid testing mEthods into Microfluidic devices for improved biosensing Applications, H2020-MSCA-RISE-2019, 2020-2024. GA No. 872662,

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NANOFACTS - Networking Activities for Nanotechnology-Facilitated Cancer Theranostics, H2020 - WIDESPREAD-05-2020 – Twinning, 2021-2024. GA No. 952259

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Development and Integration of Microfluidic Biosensors for Meat Safety monitoring in farm-to-slaughterhouse continuum (DIBMES), Innovation fond, Serbia, Call: Proof of Concept ID: 5524

Microfluidics

CISTEM- Heart on chip based on induced pluripotent stem cell technology for personalized medicine

Centar za senzorske tehnologije

Cilj projekta: CISTEM kombinuje vrhunske mikrofluidne tehnologije i koncept organa-na-čipu, zajedno sa tehnologijom indukovanih pluripotentnih matičnih ćelija, kako bi dizajnirao novu generaciju srca na čipu za lečenje pacijenta.
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O projektu: Mnoge retke bolesti uzrokuju hronične zdravstvene probleme ili su čak opasne po život. Uticaj na kvalitet života obolelih pacijenata, od kojih su mnogi deca, je značajan. Danas, sve je veći broj lekova za retke bolesti. Međutim, većina retkih bolesti još uvek nije efikasno lečena. Razvoj novih sistema za otkrivanje lekova predstavlja glavni prioritet sektora javnog zdravlja. „Organi na čipu“zasnovani na mikrofluidnoj tehnologiji predstavljaju moćno sredstvo za istraživanje mehanizama retkih bolesti i testiranje novih lekova i načina lečenja, zbog njihove sposobnosti da imitiraju arhitekturu mikro-okruženja inspirisanu funkcijama na nivou organa in vivo. Međutim, zbog varijabilnosti mehanizama retkih bolesti od jednog pacijenta do drugog, tehnologija na čipu mora biti preciznija i usmerena prema personalizovanoj medicini. Ljudski indukovane pluripotentne matične ćelije (iPSC) imaju snažan potencijal u inženjerskom organu na čipu, jer su izvedene na način prilagođen pacijentu, što ih čini izvanrednim izvorom za izgradnju ljudskih modela za personalizovani skrining lekova, kao i za razumevanje osnova bolesti specifičnih za pacijenta. Da bi pružio novu minijaturizovanu platformu za ispitivanje retkih bolesti koja je u stanju da odgovori na goruća pitanja u preciznoj medicini danas, CISTEM sinergijski povezuje nekoliko različitih oblasti koje se uglavnom na akademskom i industrijskom nivou istražuju odvojeno. CISTEM projekat se sprovodi spajanjem komplementarnih veština i kompetencija svih partnera na projektu i stvaranjem sinergije između njih.
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<img decoding="async" loading="lazy" class="alignnone wp-image-693 size-full" src="https://api.biosens.rs/wp-content/uploads/2021/06/CISTEM-Photo.png" alt="" width="924" height="587" srcset="https://api.biosens.rs/wp-content/uploads/2021/06/CISTEM-Photo.png 924w, https://api.biosens.rs/wp-content/uploads/2021/06/CISTEM-Photo-300x191.png 300w, https://api.biosens.rs/wp-content/uploads/2021/06/CISTEM-Photo-768x488.png 768w" sizes="(max-width: 924px) 100vw, 924px" /><img decoding="async" loading="lazy" class="alignnone wp-image-695 size-full" src="https://api.biosens.rs/wp-content/uploads/2021/06/CISTEM-Photo.jpg" alt="" width="845" height="321" srcset="https://api.biosens.rs/wp-content/uploads/2021/06/CISTEM-Photo.jpg 845w, https://api.biosens.rs/wp-content/uploads/2021/06/CISTEM-Photo-300x114.jpg 300w, https://api.biosens.rs/wp-content/uploads/2021/06/CISTEM-Photo-768x292.jpg 768w" sizes="(max-width: 845px) 100vw, 845px" />

Naučni saradnik u oblasti tehničko–tehnoloških i biotehničkih nauka

Dr Radonić je doktorirao iz oblasti elektronike 2010. godine na Univerzitetu u Novom Sadu. Autor je 2 poglavlja u knjizi, 30 radova u naučnim časopisima, više od 60 konferencijskih radova, 10 tehničkih rešenja i ima više od 440 citata. Dr Radonić je bio rukovodilac na četiri nacionalna projekta, jednom projektu koji je finansirala Evropska banka za istraživanje i razvoj, jednom bilateralnom projektu između Srbije i Slovenije, projektu koji je finansirao Good Food Institute iz SAD-a i vođa radnog paketa u jednom FP7 projektu – Innosens i jednom H2020 – Antares. Učestvovao je u brojnim istraživačkim projektima iz programa FP6, FP7, H2020, COST, Eureka, IPA. Član je grupa IEEE i MAGEO (Mikrotalasi u poljoprivredi, životnoj sredini i posmatranju Zemlje) i član tehničkih programskih odbora konferencije EUROCON (Computer as a Tool, 2015), Salamanka, Španija i Sensodevice konferencije 2019, Nica, Francuska. Član upravnog odbora Instituta BioSense od 2015-2020 i potpredsednik Naučnog veća Instituta BioSense 2015-2020. Od 2022 obavlja funkciju pomoćnika direktora za nauku. Recenzent u mnogim naučnim časopisima: Scientific Reports, Lab-on-Chip, IEEE Sensors Journal, MDPI Sensors, MDPI Electronics, Sensors and Actuators B, IEEE Wireless and component letters, Electronic letters, etc. Oblast naučnog istraživanja dr Radonića je mikrofluidika, razvoj senzora (mikrotalasni, optički, mikrofluidni senzori i biosenzori), elektronskih, RF i mikrotalasnih pasivnih komponenti (filteri, spojnica, diplekser itd.), antena i RFID sistema, periodični i veštački materijali, tehnologije fabrikacije, merenja i karakterizacija.

Jovana Stanojev je istraživač saradnik i student doktorskih studija na Tehnološkom fakultetu u Novom Sadu, na Univerzitetu u Novom Sadu. Studirala je inženjerstvo materijala i trenutno završava svoju doktorsku disertaciju. Tema njene doktorske disertacije je "Razvoj nehlađenog infracrvenog detektora na bazi olovo selenida". Njeno istraživanje se fokusira na razvoj novih materijala za senzore, nanomaterijala i nanomembrana, mikrofluidiku, uzgajanje ćelijskih kultura i razvoj platformi organ-na-čipu.

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Georges Dubourg је zaposlen u Institutu BioSens од 16. oktobra 2015. godine. Prethodno je bio zaposlen na Fakultetu tehničkih nauka, na Departmanu za energetiku, elektroniku i telekomunikacije, od septembra 2014. godine kao saradnik na FP7 projektu InnoSense. Od januara 2013. do jula 2014. godine radio je kao posle doktorski istaživač na Institutu za elektroniku, mikro i nanoelektroniku (IEMN) na Univerzitetu u Lilu (Francuska). Doktorske studije završio je na Univerzitetu u Bordou (Francuska), gde je radio kao istraćivač u laboratoriji Integracija: od Materijala do Sistema (IMS). U toku svog dosadašnjeg rada, dr Georges Dubourg se prvenstveno bavio naučnim istaživanjima u oblasti mikroelektronike i mikrofabrikacionih tehnologija, gde je ostvario zapažene rezultate na međunarodnom nivou. Takođe, učestvovao je u izvođenju auditornih i laboratorijskih vežbi kao asistent u nastavi od 2010. do 2012. na Univerzitetu u Bordou (Francuska) na nekoliko predmeta iz oblasti elektronike i tehnologija mikrofabrikacije na osnovnim akademskim i master studijama. Sarađivao je sa studentima pri izradi obaveznih projekata, diplomskih i master završnih radova. Domen istraživanja obuhvataju razvoj i fabrikaciju fleksibilnih senzora, mikrofluidiku i razvoj platformi organ-na-čipu. Georges je koordinator Horizon2020 projekta CISTEM-GA 778354.

Ovaj projekat je finansiran od strane Evropske Unije, iz programa za Istraživanje i Inovacije-Horizont 2020, pod sporazumom o grantu broj 778354. 

            


Horizont 2020

Srce-na-čipu baziran na tehnologiji indukovanih pluripotentnih matičnih ćelija za primenu u lečenju i terapiji pacijenata.

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Portabilni maseni spektrometar sa kvadropolnim analizatorom za istraživanje efekata uzimanja hrane kod potrošača radi personalizovane nutritivnosti

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Zaštita kože od UV zračenja bazirana na nanomaterijalima

Selektivna terapija kancera bazirana na sporom otpuštanju lekova iz silikatnih nanomaterijala

Primene u medicini

Mikrofluidika je oblast koja se bavi manipulacijom i kontrolom malih količina fluida u mikro- i nano-kanalima i kao takva mikrofludika je napravila pravu malu revoluciju u mikrobiološkim, medincinskim i hemijskim analizama.

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Naša istraživanja su fokusirana na analizu i projektovanje različitih mikrofludičinih komponenta, razvoj novih materijala za mikrofluidične čipove i integraciju mikrofluidike sa različitim sistemima za detekciju i elektronikom. U tu svrhu koristimo različite tehnologije fabrikacije, kao što su Polidimetilsiloksan (PDMS), niskotemperaturna zajedno pečena keramika (LTCC), 3D, inkdžet i bio-štampa, ksirografija, kao i hibridne tehnologije.
Kako korišćenje samo jedne tehnologije fabrikacije ne može da ispuni sve zahteve savremenih mikrofluidičnih sistema, koristimo hibridni pristup - kombinujemo različite tehnologije sa različitim materijalima da bismo dobili mikrofluidične čipove sa visokom rezolucijom, malim dimenzijama i visokim protocima. Na primer, hibridni pristup se koristi za razvoj višeslojnih čipova za filtraciju, mešača fluida za visoke protoke, kao i separatora za polen.

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Takođe, integrišemo mikrofluidne čipove sa reagentima za analizu na čipu sa različitim tipovima senzora u cilju razvoja labratorije na čipu za detekciju parametara fluida, koji nalaze primenu u kontroli mešanja fluida, biomedicine, monitoringu ekosistema i analizi tečne hrane.
Osim toga, radimo na razvoju mikrofluidičnog reaktora, koji integriše sistem za ćelijsku kulturu, različite senzore za detekciju koncentracije hranljivih materija i merenje metaboličkih otpadnih proizvoda, optičku detekciju i elektroniku u jedan jedinstveni sistem na čipu.

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Prateći ovaj koncept, razvijamo i mikrofluidičnu platformu koja ima mogućnost da reprodukuje dinamiku i heterogenost zemljišta na taj način razvijajući sistem za monitoring interakcije između biljke i mikrobioma.

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<img class="alignnone wp-image-1529 size-full" src="https://api.biosens.rs/wp-content/uploads/2021/06/70.jpg" alt="" width="763" height="323" />

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PROJEKTI:

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CISTEM - Heart On chip based on induced pluripotent Stem cell Technology for personalized Medicine, H2020-MSCA-RISE-2017, GA No. 778354

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REALSENSE1 - Monitoring of cell culture parameters using sensors for biomass and nutrients/metabolites in media: Lab-on-a-Chip (LOC) approach, 2019-2021; Good Food Institute Inaugural Competitive Grant Program.

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IPANEMA - Integration of PAper-based Nucleic acid testing mEthods into Microfluidic devices for improved biosensing Applications, H2020-MSCA-RISE-2019, 2020-2024. GA No. 872662,

CISTEM- Heart on chip based on induced pluripotent stem cell technology for personalized medicine

Teme:

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CST