Center for sensing technologies

Project aim: synthesis and characterization of nanocomposites consisting of cross-linked polyurethanes and mesoporous silica nanoparticles (MSNs) or porous silicon nanoparticles (pSiNps) for application as antimicrobial coatings on biomedical devices and implants.
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About the project: Bacterial infections from medical devices or implants are an increasing problem as a result of more widespread bacterial resistance caused by overuse of antibiotics, which can lead to contamination of medical devices and implants and to fatal patient outcomes. However, such bacterial contamination can be reduced by using appropriate materials to coat the surfaces of medical devices. Polyurethane Nanocomposites (PUN), with excellent mechanical properties and biocompatibility, are good candidates for use as antibacterial coatings on medical devices and implants. Also, mesoporous silicate nanoparticles (MSNs) and Mesoporous Silicon Nanoparticles (pSiNps) have specific properties, such as good biocompatibility, large specific surface area, uniform pore size distribution, ability for variously surface functionalization, excellent thermal stability and strength, meaning they are useful as controlled therapeutic molecule delivery systems
PUNs based on MSNs or pSiNps will include combinations of different antimicrobials, isolated from plants, to achieve effective and safe protection against bacterial infections. Also, in order to increase biocompatibility of films and minimize the occurrence of thromboembolic events after biomaterial implantation, the surface structure of these materials will be designed to decrease protein adsorption and to have weak cell adhesivity, i.e., to minimize blood coagulation.

Full research professor in the field of natural and mathematical sciences -chemistry

Nikola Knežević

Dr. Nikola Knežević is a Full research professor at the Center for Sensor Technologies at the Biosense Institute. He received his doctorate in 2009 at Iowa State University, USA, in synthesizing and functionalizing porous silica nanoparticles for stimuli-responsive and targeted drug delivery. He gained further research experience as a postdoctoral fellow at the University of Houston (research in the field of multistep organic synthesis), Universidad Complutense de Madrid (nanomaterials science), Institute Charles Gerhardt Montpellier (nanomaterials science), FP7 - ERA ERA Chairs postdoctoral fellow at the Vinča Nuclear Institute and as Pole Chimie Ballard Visiting Professor at the University of Montpellier. Nikola is the author/co-author of over 40 scientific publications, including 2 book chapters and one patent, with over 1900 citations and an H index of 21. Nikola is the coordinator of one H2020 project (NANOFACTS GA 952259), three Serbian national projects (PRECAST – 6060755, proof of concept – 5566, technology transfer -1135), and two bilateral research projects (with France and Germany). Research interests: Synthesis of functional bioresponsive nanomaterials for use in health protection and construction of biosensors.

Minja Mladenović is a Junior Research Assistant at the BioSense Institute and a Ph.D. student of Chemistry at the Faculty of Sciences, University of Novi Sad. She received her Bachelor (2015) and Master (2016) degrees from the Department of Chemistry, Biochemistry and Environmental Protection at the Faculty of Sciences, as well. Her research interests include synthesis, functionalization and characterization of silica-based nanomaterials and their application in stimuli-responsive and controlled drug/biomolecules delivery.

Minja Mladenović

BILATERAL

Due to their small diameter (ca. 100 nm) and highly porous nature, mesoporous silica nanoparticles (MSNs) have high specific surface area (ca. 1000 m2/g) which can be utilized for a plethora of functionalizations, loading/adsorption of different molecules and their removal, or as a platform for signaling molecules to detect different analytes.

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Mesoporous organosilicate nanomaterial (also termed Periodic Mesoporous Organosilica (PMO)) contains a large percentage of organic groups within the nanosphere, exhibiting unique characteristics for different applications.

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Our research is being conducted toward design of new series of MSN and PMO nanomaterials, as well as their core/shell analogs.

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Surface of these nanomaterials is being modified with different molecules to specifically interact with desired tissues, pollutants, or analytes, for their efficient application in monitoring and protection of health, food and the environment.

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One of the research goals is to develop smart nanomaterials with stimuli-responsive release of MSN/PMO-loaded molecules for diagnostics, imaging, and therapy of cancer tissues.

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Polymer-MSN composite materials are also being developed for applications as antimicrobial smart food packaging and medical implants.

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Finally, PMO nanoparticles are being constructed for efficient UVA and UVB blocking and application in a safer generation of sunscreens.

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<img class="alignnone wp-image-1626 size-full" src="https://api.biosens.rs/wp-content/uploads/2021/06/slika-1.png" alt="" width="837" height="466" />

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<a href="https://api.biosens.rs/wp-content/uploads/2021/06/68.tif"><img class="alignnone size-medium wp-image-1526" src="https://api.biosens.rs/wp-content/uploads/2021/06/68.tif" alt="" width="1" height="1" /></a>

Projects:

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PRECAST: Prediction of Cancer Treatment Effectiveness with Stimuli-responsive Nanomaterials, Science Fund of the Republic of Serbia

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<a href="https://cordis.europa.eu/project/id/952259">NANOFACTS: Networking Activities for Nanotechnology-Facilitated Cancer Theranostics H2020-WIDESPREAD-2020-5 </a>

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Novel Active Components for Health- and Environment-friendly UV-Blocking Based on Enriched Organosilica Nanomaterials, Project ID: 5566, The Innovation Fund of the Republic of Serbia

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III 44006, Ministry of Education, Science, and Technological Development of the Republic of Serbia.

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Silica nanomaterials

Heart-on-chip based on induced pluripotent stem cell technology for patient treatment

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Portable mass spectrometer based on a quadrupole analyser for investigaiton of consumer food intake effects for personalised nutrition using.

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Skin protection from UV irradiation based on nanoparticles

Selective cancer therapy based on slow release of drugs from silica nanomaterials

Medical and healthcare applications

Composite films and coatings utilize the combined properties of more than one material, opening up the possibility for realization of materials in the form of film with particular characteristics, which consequently can be employed for various applications such as gas sensing, piezoelectric and magnetic field sensors, smart packaging, protective coatings, etc.

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Our research focuses on development of composite films to be applied in colorimetric gas sensors. Namely, we investigate transparent hydrophobic polymer matrices with conductive metallic or metal-oxide particles. Such structures exhibit Local Surface Plasmon Resonance effect, and due to their properties and responsiveness to gases, these composite films are applied in the development of fast and durable sensors for gas monitoring in parts-per-million range.

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Second research direction involves application of biopolymer matrices as a part of composite systems. We are developing pH-sensitive composite films based on edible biopolymer and food extracted dyes, to be employed as a smart packaging solution for detection of food spoilage. In addition, we are developing fruit coatings based on biopolymers matrices and essential oil droplets to act as a physical and gas barrier with antimicrobial properties. These coatings extend fruit shelf life by preventing moisture loss and growth of fungi, while being edible, odorless, and entirely transparent.

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<img class="alignnone wp-image-1516 size-full" src="https://api.biosens.rs/wp-content/uploads/2021/06/63.png" alt="" width="1084" height="387" />

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Projects:

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III 44006 Development of new information and communication technologies, based on advanced mathematical methods, with applications in medicine, telecommunications, power systems, protection of national heritage and education, Ministry of Education, Science, and Technological Development of the Republic of Serbia.

Composite films

Biocompatible nanocomposites based on polyurethane networks and mesoporous silica nanoparticles for application as antimicrobial coatings for medical devices and implants

Centar za senzorske tehnologije

Cilj projekta: sinteza i karakterizacija nanokompozita sastavljenih od umreženih poliuretana i mezoporoznih silikatnih nanočestica (MSN) ili poroznih silicijumskih nanočestica (pSiNps) za potencijalnu primenu u obliku antimikrobnih prevlaka za biomedicinske uređaje i implante.
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O projektu: 
Bakterijske infekcije na medicinskim uređajima predstavljaju sve veći problem usled povećane rezistencije bakterija na tretman, prouzrokovane preteranom upotrebom antibiotika, što može dovesti do kontaminacije medicinskih uređaja i implanata i fatalnih zdravstvenih posledica. Međutim, takve kontaminacije bakterijama mogu biti smanjene koristeći odgovarajuće materijale kao prevlake za medicinske uređaje. Poliuretanski nanokompoziti (PUN) su dobri kandidati za primenu u obliku antibakterisjkih prevlaka za biomedicinske uređaje i implante zahvaljujući dobrim mehaničkim svojstvima i dobroj biokompatibilnosti. Pored toga, istraživanja u oblasti mezoporoznih silikatnih nanočestica (MSN) i mezoporoznih silicijumskih nanočestica (pSiNps) su privukla veliku pažnju istraživača posebno zbog mogućnosti kontrolisane isporuke terapeutskih molekula zbog specifičnih svojstava MSN ili pSiNps nanočestica, kao što su: dobra biokompatibilnost, velika specifična površina, poroznost sa ujednačenom raspodelom veličina pora, mogućnost raznovrsne funkcionalizacije površine, visoka termička stabilnost i čvrstoća.
Ispitivaće se PUN na bazi MSN ili pSiNps koje bi sadržale kombinacije različitih antimikrobnih molekula, izolovanih iz biljaka, radi postizanja efikasne i bezbedne zaštite od bakterijskih infekcija. Takođe, kako bi se povećala biokompatibilnost filmova i minimizirala pojava tromboembolijskih događaja nakon implantacije biomaterijala, površinska struktura ovih materijala će biti konstruisana sa ciljem u što manjoj adsorpciji proteina i što slabijoj adheziji ćelija, odnosno ka minimiziranju mogućnosti koagulacije krvi.

Naučni savetnik u oblasti prirodno–matematičkih nauka -hemija

Dr Nikola Knežević je Naučni savetnik u Centru za senzorske tehnologije na Institutu Biosens. Doktorirao je 2009. godine na Iowa State University, SAD, iz oblasti sinteze i funkcionalizacije poroznih silikatnih nanočestica za stimulans-responsivnu i ciljajuću isporuku lekova. Dalje istraživačko iskustvo stekao je kao postdoktorand na Univerzitetu u Hjustonu (istraživanje iz oblasti višestepene organske sinteze), Universidad Complutense de Madrid (nauka o nanomaterijalima), Institute Charles Gerhardt Montpellier (nauka o nanomaterijalima), FP7 – ERA ERA Chairs postdoktorand u Nuklearnom institutu Vinča i kao Pole Chimie Balard gostujući profesor na Univerzitetu u Monpeljeu. Nikola je autor/koautor na preko 40 naučnih publikacija, uključujući 2 poglavlja u knjigama i jedan patent, sa preko 1900 citata i H indeksom 21. Nikola je koordinator jednog projekta H2020 (NANOFACTS GA 952259), tri srpska nacionalna projekta (PRECAST – 6060755, dokaz koncepta – 5566, transfer tehnologija -1135) i dva bilateralna istraživačka projekta (sa Francuskom i Nemačkom). Istraživačka interesovanja: sinteza funkcionalnih bioresponsivnih nanomaterijala za primenu u zaštiti zdravlja i konstrukciji biosenzora.

Minja Mladenović je zaposlena na Institutu BioSens kao istraživač-pripravnik. Takođe, student je doktorskih akademskih studija hemije na Prirodno-matematičkom fakultetu Univerziteta u Novom Sadu, gde je završila osnovne i master studije hemije. Bavi se naučno-istraživačkim radom u oblasti sinteze, funkcionalizacije i karakterizacije nanomaterijala na bazi silike, kao i primenom istih za stimulisanu i kontrolisanu isporuku lekova i biomolekula.

BILATERALNI

Srce-na-čipu baziran na tehnologiji indukovanih pluripotentnih matičnih ćelija za primenu u lečenju i terapiji pacijenata.

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Portabilni maseni spektrometar sa kvadropolnim analizatorom za istraživanje efekata uzimanja hrane kod potrošača radi personalizovane nutritivnosti

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Zaštita kože od UV zračenja bazirana na nanomaterijalima

Selektivna terapija kancera bazirana na sporom otpuštanju lekova iz silikatnih nanomaterijala

Primene u medicini

Kompozitni filmovi sadrže kombinovana svojstva više od jednog materijala, čime otvaraju mogućnost realizacije materijala u formi filma sa naročitim karakteristikama, koji opet mogu biti primenjeni u razne svrhe kao sto su gasni senzori, piezoelektrični i senzori magnetnog polja, pametna pakovanja, zaštitne prevlake itd.

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Deo naših istraživanja fokusira se na razvoj kompozitnih filmova za primenu u kolorimetrijskim gasnim senzorima. Naime, mi istražujemo transparentne hidrofobne polimerne matrice sa provodnim metalnim i metal-oksidnim česticama. Takve strukture pokazuju Lokalnu Površinsku Plazmonsku Rezonanciju (LPPR), i zahvaljujući njihovim svojstvima i osetljivosti na gasove, ovi kompozitni filmovi mogu biti primenjeni u razvoju brzih i postojanih gasnih senzora za praćenje koncentracije gasova u ppm opsegu.

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Drugi smer istraživanja bazira se na primeni biopolimernih matrica kao dela kompozitnih sistema. Razvijamo pH osetljive kompozitne filmove na bazi biokompatibilnih polimera i boja ekstrahovanih iz hrane, za primenu u pametnim pakovanjima kao vizuelni indikator koji pruža informaciju o potencijalnom kvarenju hrane. Pored ovoga, radimo na razvoju prevlaka za voće na bazi biopolimernih matrica i esencijalnog ulja sa ulogom fizičke i gasne barijere koja poseduje antimikrobna svojstva. Ove prevlake produžavaju svežinu ploda putem sprečavanja gubitka vode i prevencije pojave gljivica i plesni, a uz to su jestive, bez mirisa i ukusa i potpuno transparentne.

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<img class="alignnone wp-image-1516 size-full" src="https://api.biosens.rs/wp-content/uploads/2021/06/63.png" alt="" width="1084" height="387" />

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Projekat:

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III 44006 Razvoj novih informaciono-komunikacionih tehnologija, korišćenjem naprednih matematičkih metoda, sa primenama u medicini, telekomunikacijama, energetici, zaštiti nacionalne baštine i obrazovanju, Ministarstvo prosvete, nauke i tehnološkog razvoja Republike Srbije.

Kompozitni filmovi

Zbog malog prečnika (oko 100 nm) i veoma porozne prirode, mezoporozne silikatne nanočestice (MSN) imaju veliku specifičnu površinu (oko 1000 m2 / g) koja se može koristiti za različite funkcionalizacije, punjenje/adsorpciju različitih molekula i njihovo uklanjanje, ili kao platforma za signalne molekule za detekciju različitih analita.

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Mezoporozni organosilikatni nanomaterijal (Periodični mezoporozni organosilikat (PMO)) sadrži veliki procenat organskih grupa, što omogućuje da ispoljavaju jedinstvene karakteristike za različite primene.

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Naše istraživanje se provodi u cilju dizajniranja novih serija MSN i PMO nanomaterijala, kao i njihovih jezgro / ljuska analoga.

Površina ovih nanomaterijala se modifikuje različitim molekulima kako bi specifično stupila u interakciju sa željenim tkivima, zagađivačima ili analitima, radi njihove efikasne primene u praćenju i zaštiti zdravlja, hrane i životne sredine.

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Jedan od ciljeva istraživanja je razvoj pametnih nanomaterijala sa stimulans-responzivnim oslobađanjem molekula iz MSN / PMO materijala, sa primenom u dijagnostici, snimanju i terapiji tkiva raka.
Kompozitni materijali polimer-MSN se takođe razvijaju u cilju primene kao antimikrobnih pametnih ambalaža za hranu i medicinskih implanata.

Konačno, nove PMO nanočestice se konstruišu radi efikasnog blokiranja UVA i UVB zračenja i primene u bezbednijoj generaciji krema za sunčanje.

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<img class="alignnone wp-image-1626 size-full" src="https://api.biosens.rs/wp-content/uploads/2021/06/slika-1.png" alt="" width="837" height="466" />

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Projekti:

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PRECAST: Prediction of Cancer Treatment Effectiveness with Stimuli-responsive Nanomaterials, Science Fund of the Republic of Serbia

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<a href="https://cordis.europa.eu/project/id/952259">NANOFACTS: Networking Activities for Nanotechnology-Facilitated Cancer Theranostics H2020-WIDESPREAD-2020-5</a>

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Novel Active Components for Health- and Enviroment-friendly UV-Blocking Based on Enriched Organosilica Nanomaterials, Project ID: 5566, The Innovation Fund of the Republic of Serbia

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III 44006 Razvoj novih informaciono-komunikacionih tehnologija, korišćenjem naprednih matematičkih metoda, sa primenama u medicini, telekomunikacijama, energetici, zaštiti nacionalne baštine i obrazovanju, Ministarstvo prosvete, nauke i tehnološkog razvoja Republike Srbije.

Silikatni nanomaterijali

Biokompatibilni nanokompoziti na bazi poliuretanskih mreža i mezoporoznih silikatnih nanočestica za primenu u obliku antimikrobnih prevlaka medicinskih uređaja i implanata

Biokompatibilni nanokompoziti na bazi poliuretanskih mreža i mezoporoznih silikatnih nanočestica za primenu u obliku antimikrobnih prevlaka medicinskih uređaja i implanata

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CST