Center for sensing technologies

Project aim: The project aims at developing novel brain-cancer targeting nanomaterials as contrast agents for application in efficient magnetic resonance imaging (MRI) of Glioblastoma multiforme (GBM).
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About the project: In case of successful cancer-targeting these novel nanoparticles would enable strong contrast enhancement, selectively at the tumor location. Upon testing different structures of nanomaterials, the methodology could also reveal the most effective composition of a nanomedicine for potent GBM cancer targeting and personalized therapy. Different types of nanomaterials based on mesoporous silica nanoparticles (MSN) are to be developed for GBM imaging and selective drug-delivery in response to weakly acidic extracellular environment of GBM cancer tissues.
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Biosense institute is involved in synthesis and development of the nanomaterials while part of the instrumental characterizations is to be conducted at the partnering institution, Faculty of Technology and Metallurgy, University of Belgrade. The initial testing of MRI capabilities of the nanomaterials is to be performed at the project-supporting BioNanoTechnology laboratory at the Wageningen University and Research, the Netherlands while experiments on mice are to be conducted at the other project-supporting institution, Trinity College Dublin, Ireland.
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<img decoding="async" loading="lazy" class="alignnone size-medium wp-image-1391" src="https://api.biosens.rs/wp-content/uploads/2021/06/Logo-ENG-2-300x97.png" alt="" width="300" height="97" srcset="https://api.biosens.rs/wp-content/uploads/2021/06/Logo-ENG-2-300x97.png 300w, https://api.biosens.rs/wp-content/uploads/2021/06/Logo-ENG-2.png 512w" sizes="(max-width: 300px) 100vw, 300px" />

Full research professor in the field of natural and mathematical sciences -chemistry

Nikola Knežević

Dr. Nikola Knežević is a Full research professor at the Center for Sensor Technologies at the Biosense Institute. He received his doctorate in 2009 at Iowa State University, USA, in synthesizing and functionalizing porous silica nanoparticles for stimuli-responsive and targeted drug delivery. He gained further research experience as a postdoctoral fellow at the University of Houston (research in the field of multistep organic synthesis), Universidad Complutense de Madrid (nanomaterials science), Institute Charles Gerhardt Montpellier (nanomaterials science), FP7 - ERA ERA Chairs postdoctoral fellow at the Vinča Nuclear Institute and as Pole Chimie Ballard Visiting Professor at the University of Montpellier. Nikola is the author/co-author of over 40 scientific publications, including 2 book chapters and one patent, with over 1900 citations and an H index of 21. Nikola is the coordinator of one H2020 project (NANOFACTS GA 952259), three Serbian national projects (PRECAST – 6060755, proof of concept – 5566, technology transfer -1135), and two bilateral research projects (with France and Germany). Research interests: Synthesis of functional bioresponsive nanomaterials for use in health protection and construction of biosensors.

Mirjana Mundžić has a Bachelor and Master`s degree in Biochemistry. She is currently a PhD Student of Biochemistry at the Faculty of Sciences in Novi Sad and a Junior Research Assistant at the BioSense Institute. Her main research interests are evaluation of cytotoxicity of nanomaterials along with the evaluation of their biocompatibility and bioactivity.

Mirjana Mundžić

Minja Mladenović is a Junior Research Assistant at the BioSense Institute and a Ph.D. student of Chemistry at the Faculty of Sciences, University of Novi Sad. She received her Bachelor (2015) and Master (2016) degrees from the Department of Chemistry, Biochemistry and Environmental Protection at the Faculty of Sciences, as well. Her research interests include synthesis, functionalization and characterization of silica-based nanomaterials and their application in stimuli-responsive and controlled drug/biomolecules delivery.

Minja Mladenović

Aleksandra Pavlović is a Junior Research Assistant at the BioSense Institute and a Ph.D. student of Chemistry at the Faculty of Sciences, University of Novi Sad. She received her Bachelor (2019) and Master (2020) degrees from the Department of Chemistry, Biochemistry and Environmental Protection at the Faculty of Sciences, as well. Her research interests include synthesis, functionalization and characterization of periodically mesoporous organosilicate nanoparticles for dermal use, as well as the development of biosensors for various biomolecules.

Aleksandra Pavlović

Mila Djisalov has a BSc in Biochemistry and a Master’s degree in Molecular Biology. She is currently a Ph.D. Student of Biochemistry at the Faculty of Sciences in Novi Sad and a Junior Researcher in BioSense Institute. Her main research interests are biosensor development and molecular tools for pathogen detection, cellular agriculture, and the development of lab-on-a-chip platforms. Mila is also one of the co-founders of the student group The BioSense Alt. Protein Project formed at the BioSense Institute.

MIla Đisalov

Ivana Gadjanski PhD is Research Associate Professor at BioSense Institute, University of Novi Sad, Serbia. She graduated from the Faculty of Biology, University of Belgrade as the 1st in the class (2003). She obtained PhD in Neuroscience (summa cum laude -oral presentation) from Georg-August University &amp; Max Planck Institute for Biophysical Chemistry, Goettingen, Germany (2007) and worked as a postdoctorate researcher at Ludwig-Maximillian University, Munich, Germany. Ivana then turned to the bioengineering field and spent a year as a Fulbright Visiting Scholar at Columbia University, USA - Lab for Stem cells and Tissue Engineering (Head: prof. Gordana Vunjak-Novakovic). In BioSense, she is moving further into the cellular agriculture field - developing solutions for cultivated meat. She also works on biosensing for agrifood, biomedicine and environmental monitoring. Ivana is a grantee of the Good Food Institute and coordinator of Horizon2020 projects DRAGON GA 810755 and IPANEMA GA 872662.

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Ivana Gađanski

PRECAST-LOGO

National

Due to their small diameter (ca. 100 nm) and highly porous nature, mesoporous silica nanoparticles (MSNs) have high specific surface area (ca. 1000 m2/g) which can be utilized for a plethora of functionalizations, loading/adsorption of different molecules and their removal, or as a platform for signaling molecules to detect different analytes.

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Mesoporous organosilicate nanomaterial (also termed Periodic Mesoporous Organosilica (PMO)) contains a large percentage of organic groups within the nanosphere, exhibiting unique characteristics for different applications.

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Our research is being conducted toward design of new series of MSN and PMO nanomaterials, as well as their core/shell analogs.

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Surface of these nanomaterials is being modified with different molecules to specifically interact with desired tissues, pollutants, or analytes, for their efficient application in monitoring and protection of health, food and the environment.

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One of the research goals is to develop smart nanomaterials with stimuli-responsive release of MSN/PMO-loaded molecules for diagnostics, imaging, and therapy of cancer tissues.

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Polymer-MSN composite materials are also being developed for applications as antimicrobial smart food packaging and medical implants.

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Finally, PMO nanoparticles are being constructed for efficient UVA and UVB blocking and application in a safer generation of sunscreens.

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<img class="alignnone wp-image-1626 size-full" src="https://api.biosens.rs/wp-content/uploads/2021/06/slika-1.png" alt="" width="837" height="466" />

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<a href="https://api.biosens.rs/wp-content/uploads/2021/06/68.tif"><img class="alignnone size-medium wp-image-1526" src="https://api.biosens.rs/wp-content/uploads/2021/06/68.tif" alt="" width="1" height="1" /></a>

Projects:

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PRECAST: Prediction of Cancer Treatment Effectiveness with Stimuli-responsive Nanomaterials, Science Fund of the Republic of Serbia

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<a href="https://cordis.europa.eu/project/id/952259">NANOFACTS: Networking Activities for Nanotechnology-Facilitated Cancer Theranostics H2020-WIDESPREAD-2020-5 </a>

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Novel Active Components for Health- and Environment-friendly UV-Blocking Based on Enriched Organosilica Nanomaterials, Project ID: 5566, The Innovation Fund of the Republic of Serbia

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III 44006, Ministry of Education, Science, and Technological Development of the Republic of Serbia.

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Silica nanomaterials

Heart-on-chip based on induced pluripotent stem cell technology for patient treatment

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Portable mass spectrometer based on a quadrupole analyser for investigaiton of consumer food intake effects for personalised nutrition using.

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Skin protection from UV irradiation based on nanoparticles

Selective cancer therapy based on slow release of drugs from silica nanomaterials

Medical and healthcare applications

Biomolecules, including DNA, RNA, proteins, peptides and the constellation of small molecules made by cells, are the molecules that fuel life. Biomolecular engineering (BME) is the analysis and engineering of these living systems. BME research uses techniques from synthetic biology, systems biology, biomedical research and biotechnology, biochemistry and biophysics of biological systems and cell biology to quantify and manipulate the structure and function of molecules and macromolecular assemblies for potential applications in:

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· Biotechnology: Biosensing and bioanalytical devices, microfluidics-enabled cell culture technologies, (e.g. organ-on-a-chip), development and application of novel molecular tools (applicable for food safety evaluation)

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· Fundamental processes of living systems: host pathogen interactions, cellular and subcellular organization, regulation and control of biological networks

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· Human and animal disease: design and engineering of diagnostic methodologies, cell and tissue engineering, delivery of therapeutics
In addition, BME research efforts include interdisciplinary activities, such as materials science and nano- and microelectronics, as well as computer science and applied mathematics.

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In that sense, BME is a very good example of the close multidisciplinary collaboration between the Center for Biosystems and the two other centers in BioSense Institute: Center for sensing technologies and Center for information technologies.

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Projects:

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H2020 MSCA-RISE IPANEMA GA 872662 - Integration of PAper-based Nucleic acid testing mEthods into Microfluidic devices for improved biosensing Applications

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<img class="alignnone wp-image-1421 size-full" src="https://api.biosens.rs/wp-content/uploads/2021/06/bs6.jpg" alt="" width="1100" height="624" />

Biomolecular engineering

PRECAST – Prediction of Cancer Treatment Effectiveness with Stimuli-responsive Nanomaterials

Centar za senzorske tehnologije

Cilj projekta: PRECAST projekat ima za cilj razvoj novih nanomaterijala kao kontrastnih agenasa za ciljanu magnetnu rezonantnu tomografiju (MRI) multiformnog glioblastoma (GBM).
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O projektu: U slučaju uspešnog ciljanja raka, ove nove nanočestice bi omogućile snažno pojačavanje kontrasta, selektivno na mestu tumora. Nakon ispitivanja različitih struktura nanomaterijala, metodologija bi takođe mogla otkriti najefikasniji sastav nanomedikamenta za ciljanje karcinoma GBM i personalizovanu terapiju. Biće konstruisani različiti tipovi nanomaterijala na bazi mezoporoznih silikatnih nanočestica (MSN) za GBM snimanje i selektivnu isporuku lekova kao odgovor na acidifikovano vanćelijsko mikro okruženje tkiva karcinoma GBM. 
Na Institutu BioSens radiće se sinteza, kao i razvoj nanomaterijala, dok će deo instrumentalnih karakterizacija biti izveden u partnerskoj instituciji, Tehnološko-metalurškom fakultetu Univerziteta u Beogradu. Inicijalno testiranje sposobnosti nanomaterijala za MRI će se obavljati u laboratoriji BioNanoTechnology Univerziteta u Vaheningenu iz Holandije, koja podržava projekat. In vivo eksperimenti će biti sprovedeni u drugoj instituciji koja podržava projekat, Triniti koledžu u Dablinu.
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<img decoding="async" loading="lazy" class="alignnone wp-image-1390 size-medium" src="https://api.biosens.rs/wp-content/uploads/2021/06/Fond-za-nauku-logo-za-oba-projekta-300x101.png" alt="" width="300" height="101" srcset="https://api.biosens.rs/wp-content/uploads/2021/06/Fond-za-nauku-logo-za-oba-projekta-300x101.png 300w, https://api.biosens.rs/wp-content/uploads/2021/06/Fond-za-nauku-logo-za-oba-projekta.png 512w" sizes="(max-width: 300px) 100vw, 300px" />

Naučni savetnik u oblasti prirodno–matematičkih nauka -hemija

Mirjana Mundžić je završila osnovne i master studije iz biohemije. Trenutno je doktorand biohemije na Prirodno-matematičkom fakultetu u Novom Sadu i istraživač pripravnik na Institutu BioSens. Njeno glavno istraživačko interesovanje je procena citotoksičnosti nanomaterijala zajedno sa procenom njihove biokompatibilnosti i bioaktivnosti.

Dr Nikola Knežević je Naučni savetnik u Centru za senzorske tehnologije na Institutu Biosens. Doktorirao je 2009. godine na Iowa State University, SAD, iz oblasti sinteze i funkcionalizacije poroznih silikatnih nanočestica za stimulans-responsivnu i ciljajuću isporuku lekova. Dalje istraživačko iskustvo stekao je kao postdoktorand na Univerzitetu u Hjustonu (istraživanje iz oblasti višestepene organske sinteze), Universidad Complutense de Madrid (nauka o nanomaterijalima), Institute Charles Gerhardt Montpellier (nauka o nanomaterijalima), FP7 – ERA ERA Chairs postdoktorand u Nuklearnom institutu Vinča i kao Pole Chimie Balard gostujući profesor na Univerzitetu u Monpeljeu. Nikola je autor/koautor na preko 40 naučnih publikacija, uključujući 2 poglavlja u knjigama i jedan patent, sa preko 1900 citata i H indeksom 21. Nikola je koordinator jednog projekta H2020 (NANOFACTS GA 952259), tri srpska nacionalna projekta (PRECAST – 6060755, dokaz koncepta – 5566, transfer tehnologija -1135) i dva bilateralna istraživačka projekta (sa Francuskom i Nemačkom). Istraživačka interesovanja: sinteza funkcionalnih bioresponsivnih nanomaterijala za primenu u zaštiti zdravlja i konstrukciji biosenzora.

Minja Mladenović je zaposlena na Institutu BioSens kao istraživač-pripravnik. Takođe, student je doktorskih akademskih studija hemije na Prirodno-matematičkom fakultetu Univerziteta u Novom Sadu, gde je završila osnovne i master studije hemije. Bavi se naučno-istraživačkim radom u oblasti sinteze, funkcionalizacije i karakterizacije nanomaterijala na bazi silike, kao i primenom istih za stimulisanu i kontrolisanu isporuku lekova i biomolekula.

Aleksandra Pavlović je zaposlena na Institutu BioSens kao istraživač-pripravnik. Takođe, student je doktorskih akademskih studija hemije na Prirodno-matematičkom fakultetu Univerziteta u Novom Sadu, gde je završila osnovne (2019.) i master (2020.) studije hemije. Bavi se naučno-istraživačkim radom u oblasti sinteze, funkcionalizacije i karakterizacije periodično mezoporoznih organosilikatnih nanočestica za dermalnu upotrebu, kao i razvijanjem biosenzora za različite biomolekule.

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Mila Đisalov je diplomirani biohemičar i master molekularne biologije. Trenutno je student doktorskih studija biohemije na Prirodno-matematičkom fakultetu u Novom Sadu i istraživač-pripravnik na Institutu BioSens. Njena glavna istraživačka interesovanja vezana su za razvoj biosenzora i molekularnih alata za detekciju patogena, ćelijska poljoprivreda i razvoj laboratorija na čipu. Mila je takođe jedan od osnivača studentske grupe The BioSense Alt. Protein Project, osnovane na Institutu BioSens.

Mila Đisalov

Dr Ivana Gađanski je viši naučni saradnik na Institutu Biosens, Univerzitet u Novom Sadu, Srbija. Diplomirala je na Biološkom fakultetu Univerziteta u Beogradu kao prva u klasi (2003). Doktorirala je iz neuronauka (ocena summa cum laude – usmena odbrana) na Georg-August Univerzitetu i Institutu za biofizičku hemiju Maks Plank, Getingen, Nemačka (2007) i radila na posledoktorskim studijama na Univerzitetu Ludvig-Maksimilijian, Minhen, Nemačka. Ivana se zatim posvetila oblasti bioinženjerstva i provela godinu dana kao Fulbrajt stipendista – gostujući istraživač na Univerzitetu Kolumbija, Njujork, SAD u Laboratoriji za matične ćelije i tkivno inženjerstvo (rukovodilac: prof. Gordana Vunjak-Novaković). U BioSensu radi na bioinženjerstvu u oblasti ćelijske poljoprivrede - razvija rešenja za kultivisano meso. Takođe radi na razvoju biosenzora za monitoring u oblasti poljoprivrede, biomedicine i zaštite životne sredine. Ivana je stipendista Instituta za dobru hranu i koordinator Horizont2020 projekata DRAGON GA 810755 i IPANEMA GA 872662.

Nacionalni

Srce-na-čipu baziran na tehnologiji indukovanih pluripotentnih matičnih ćelija za primenu u lečenju i terapiji pacijenata.

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Portabilni maseni spektrometar sa kvadropolnim analizatorom za istraživanje efekata uzimanja hrane kod potrošača radi personalizovane nutritivnosti

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Zaštita kože od UV zračenja bazirana na nanomaterijalima

Selektivna terapija kancera bazirana na sporom otpuštanju lekova iz silikatnih nanomaterijala

Primene u medicini

Biomolekuli su molekuli su koji omogućavaju funkcionisanje živih organizama, uključujući DNK, RNK, proteine, peptide i konstelaciju malih molekula koji se sintetišu u ćelijama. Biomolekularno inženjerstvo (BMI) obuhvata ispitivanje i inženjerstvo svih ovih živih sistema. BMI istraživanja koriste tehnike i metode iz sintetičke biologije, sistemske biologije, biomedicinskih istraživanja i biotehnologije, biohemije i biofizike bioloških sistema i ćelijske biologije za kvantifikovanje i manipulisanje strukturom i funkcijom molekula i makromolekularnih sklopova za potencijalne primene u sledećim oblastima:

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· Biotehnologija: Biosenzorski i bioanalitički uređaji, tehnologije ćelijskih kultura sa omogućenom mikrofluidikom (npr. organi na čipu), razvoj i primena novih molekularnih alata detekcije patogena (primenljivo za procenu bezbednosti hrane)

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· Osnovni procesi živih sistema: interakcije patogena i domaćina, ćelijska i unutarćelijska organizacija

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· Bolesti ljudi i životinja: dizajn i inženjerstvo dijagnostičkih metodologija, ćelijsko i tkivno inženjerstvo
Pored toga, BMI istraživanja uključuju veoma interdisciplinarne aktivnosti, kao što su nauka o materijalima i nano- i mikroelektronika, kao i računarstvo i primenjena matematika. U tom smislu, BMI je vrlo dobar primer bliske multidisciplinarne saradnje između Centra za biosisteme i druga dva centra u okviru BioSens Instituta - Centra za senzorske tehnologije i Centra za informacione tehnologije.

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Projekti:

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H2020 MSCA-RISE IPANEMA GA 872662 - Integration of PAper-based Nucleic acid testing mEthods into Microfluidic devices for improved biosensing Applications

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<img class="alignnone wp-image-1421 size-full" src="https://api.biosens.rs/wp-content/uploads/2021/06/bs6.jpg" alt="" width="1100" height="624" />

Biomolekularno inženjerstvo

Zbog malog prečnika (oko 100 nm) i veoma porozne prirode, mezoporozne silikatne nanočestice (MSN) imaju veliku specifičnu površinu (oko 1000 m2 / g) koja se može koristiti za različite funkcionalizacije, punjenje/adsorpciju različitih molekula i njihovo uklanjanje, ili kao platforma za signalne molekule za detekciju različitih analita.

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Mezoporozni organosilikatni nanomaterijal (Periodični mezoporozni organosilikat (PMO)) sadrži veliki procenat organskih grupa, što omogućuje da ispoljavaju jedinstvene karakteristike za različite primene.

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Naše istraživanje se provodi u cilju dizajniranja novih serija MSN i PMO nanomaterijala, kao i njihovih jezgro / ljuska analoga.

Površina ovih nanomaterijala se modifikuje različitim molekulima kako bi specifično stupila u interakciju sa željenim tkivima, zagađivačima ili analitima, radi njihove efikasne primene u praćenju i zaštiti zdravlja, hrane i životne sredine.

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Jedan od ciljeva istraživanja je razvoj pametnih nanomaterijala sa stimulans-responzivnim oslobađanjem molekula iz MSN / PMO materijala, sa primenom u dijagnostici, snimanju i terapiji tkiva raka.
Kompozitni materijali polimer-MSN se takođe razvijaju u cilju primene kao antimikrobnih pametnih ambalaža za hranu i medicinskih implanata.

Konačno, nove PMO nanočestice se konstruišu radi efikasnog blokiranja UVA i UVB zračenja i primene u bezbednijoj generaciji krema za sunčanje.

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<img class="alignnone wp-image-1626 size-full" src="https://api.biosens.rs/wp-content/uploads/2021/06/slika-1.png" alt="" width="837" height="466" />

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Projekti:

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PRECAST: Prediction of Cancer Treatment Effectiveness with Stimuli-responsive Nanomaterials, Science Fund of the Republic of Serbia

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<a href="https://cordis.europa.eu/project/id/952259">NANOFACTS: Networking Activities for Nanotechnology-Facilitated Cancer Theranostics H2020-WIDESPREAD-2020-5</a>

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Novel Active Components for Health- and Enviroment-friendly UV-Blocking Based on Enriched Organosilica Nanomaterials, Project ID: 5566, The Innovation Fund of the Republic of Serbia

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III 44006 Razvoj novih informaciono-komunikacionih tehnologija, korišćenjem naprednih matematičkih metoda, sa primenama u medicini, telekomunikacijama, energetici, zaštiti nacionalne baštine i obrazovanju, Ministarstvo prosvete, nauke i tehnološkog razvoja Republike Srbije.

PRECAST- Prediction of Cancer Treatment Effectiveness with Stimuli-responsive Nanomaterials

Teme:

Ljudi:

Centri:

CST