Center for sensing technologies

Project aim: MicroLabAptaSens intends to make innovations in biosensor development for multiple pathogens detection for easy and fast detection in food safety, environmental monitoring, and healthcare.
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About the project: Foodborne bacterial diseases are a growing public health concern at the global level and one of the most important requirements in food safety is the development of accurate and early diagnostic tools for foodborne diseases. Project MicroLabAptaSens exploits complementary knowledge and expertise of the excellent scientists in order to: 1) Perform cutting edge research in the multidisciplinary field of sensors, microfluidics, nanomaterials, biotechnology and food safety with a main objective to 2) Develop innovative low-cost sensing devices for detection of the 3 most foodborne bacteria (Listeria monocytogenes, Salmonella enteritidis and Campylobacter jejuni) applicable for the in-situ point-of-need (PON) use and to 3) Fabricate and validate microfluidic (MF) Lab-on-a-Chip (LoC) tests with integrated sensor and on-chip reagent storages, finally aiming to 4) Pursue real-life applications in agrifood and biotech sectors. Novelty relies on a novel class of nanomaterial-functionalized aptamer-based biosensors suitable for integration into unique low-cost LoC MF tests. Application of advanced micro- and nano-technologies will allow integration with reagents for multiple pathogen analysis into user-friendly fast tests which will not require costly lab equipment, trained personnel, and special conditions. Such tests fit all the ASSURED (Affordable, Sensitive, Specific, User-friendly, Rapid and robust, Equipment-free and Deliverable to end-users) criteria recommended by the World Health Organization (WHO). The electronic readout module and smartphone application will be developed for data processing and visualization. The final goal is a cost-efficient sensing device that can satisfy market-needs and MF tests for applications in healthcare, environment monitoring and food safety sectors and can be easily scaled-up to the mass production or adapted for other specific applications such as viral or bacterial airborne pathogens detection.

Principal Research Fellow 

Vasa Radonić

Ivana Milošević, a Ph.D. student of Biotechnology at the Faculty of Technology, University of Novi Sad, is a Junior Research Assistant at the BioSense Institute. Since January 2023, Ivana has started working in the Sensing Technology team, specializing in developing biosensors based on flagellar filaments. Her work focuses on enhancing the sensitivity of these biosensors for detecting heavy metals, particularly nickel (Ni). Ivana's contributions extend to modifying electrochemical biosensors, showcasing her commitment to advancing biotechnological innovations.

Ivana Milošević

Dr Radonic received a PhD in electronics in 2010 at University of Novi Sad. He authored and co-authored 2 book chapters, 30 journal papers, more than 60 conference papers, 10 technical solutions, and has more than 440 citations. Dr Radonić was the project leader in four Serbian national projects, one project financed by European Bank for Research and Development, one Bilateral project between Serbia and Slovenia, project financed by Good Food Institute, USA and WP leader in one FP7 project–InnoSense and one H2020 project–ANTARES. He participates in a number of research FP6, FP7, H2020, COST, EUREKA, IPA projects. He is a member of IEEE and MAGEO (Microwaves in Agriculture, Environment and Earth Observation) groups and was a member of Technical Program Committees of EUROCON (Computer as Tool, 2015) conference, Salamanca, Spain and Sensordevices conference 2019, Nice, France. Steering committee member of the BioSense Institute 2015-2020, and the vice president of Scientific council of the BioSense Institute 2015-2020. From 2022, he is the assistant director for science. Reviewer in different scientific journals: Scientific Reports, Lab-on-Chip, IEEE Sensors Journal, MDPI Sensors, MDPI Electronics, Sensors and Actuators B, IEEE Wireless and component letters, Electronic letters, etc. The main expertise of Dr Radonic is microfluidics, sensors development (microwave, optical, microfluidic sensors and biosensors), electronic, RF and microwave passive components (filters, coupler, diplexer, etc.), antennas and RFID systems, periodical and artificial materials, fabrication technologies, measurements and characterization.

Stefan Jarić is employed at the Center for Sensing Technologies as a Research Assistant and a final-year PhD student in materials physics at the Department of Physics, Faculty of Sciences at the University of Novi Sad. Stefan works on the development and research of new solutions in the field of biosensors based on graphene field effect transistors and specific synthetic receptors (aptamers) for the detection of various analytes in the area of environmental protection (toxins) and medicine (biomarkers). His expertise is developing in the direction of micro and nanofabrication of sensor platforms (photolithography, physical vapor deposition of materials), characterization of materials (Raman spectroscopy, atomic force microscopy) and electrical characteristics of biosensors.

Stefan Jarić

Slavica Savić obtained her BSc degree in 2002. from the Faculty of Physical Chemistry, University of Belgrade. In 2009 she received her PhD degree in Materials in electronics, Faculty of Technical Sciences, University of Novi Sad. From Jan 2003-Sept. 2008 she worked at Institute of Technical Sciences of the Serbian Academy of Science and Arts as research assistant. From Sept. 2008.-July 2015 she worked as research assistant (2008-2010), research associate (2010- March 2015) and senior research associate (April 2015-July 2015) at Department of Material Science at Institute for Multidisciplinary Research, University of Belgrade. From July 2012 to December 2012 she was a visiting research scientist at Pontificia Universidade Catolica (PUC) Rio de Janeiro, Brasil. From July 2015-July 2020 she was senior research associate at BioSense Institute. Since July 2020 she is principal research fellow at BioSense Institute.Her research interest include advanced ceramic materials processing and characterization, materials science solid-state chemistry and physics. Specific field of research activity: Synthesis and characterisation of Eu, Zr, Nb and Ta - doped BCY10 cermets as electrolytes for IT-SOFC; Synthesis and characterization of thermistor material NiMn2O4 obtained mechanochemically and by complex polimerization method. Thermoelectric materials: Mechanochemically assisted solid state synthesis of pure NaCo2O4 and doped with Cu; Synthesis of NaCo2O4 by citrate gel process. Current activities are focused to the synthesis and characterization of MOx (M=Ti, Sn, Zn) for applications in gas sensors and humidity sensors. The significance of her research is confirmed with more than 50 scientific papers in relevant scientific journals.

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Slavica Savić

Dr Nikolina Janković (F) senior researcher in the Center for Sensing Technologies in the BioSense Institute. Author and co-author of more than 40 journal and conference papers and 5 book chapters. Main research interest metamaterials, plasmonics, acoustics, sensors, and microwave passive devices. The coordinator of the H2020 MSCA-RISE project NOCTURNO and EUREKA project WaQUMoS in Serbia. Well versed in EU regulations and procedures and the EC funded projects. In the last seven years, participated in two FP7 projects: MultiWaveS and InnoSense (Financial and Administrative Manager and WP leader), as well as in three H2020 projects (H2020 Teaming ANTARES, NOCTURNO, and SHealthy).

Nikolina Janković

Dr Marko Radović graduated in 2005 from the Faculty of Physics, University of Belgrade. In 2008 he received a MSc degree at the Faculty of Physics, University of Belgrade. He received PhD title in 2015 also at Faculty of Physics, University of Belgrade. From April 2006. to February of 2017. He was employed at the Institute of Physics in Belgrade. Since February 2017. He is employed as a senior researcher at BioSense Institute. Dr Radović research interests are focused on a bottom-up approach, involving synthesis, characterization and functionalization of nanomaterials to design of gas monitoring platforms, biosensors, optoelectronic and photocatalytic devices. He has published 25 scientific papers in international and domestic journals and he is a member of the Serbian Society for Ceramic Materials.

Marko Radović

Dr. Ivana Kundacina is a Research Associate at the BioSense Institute. She completed her bachelor’s and master’s degrees in physics and earned a Ph.D. in Applied Physics from the Faculty of Sciences, University of Novi Sad, Serbia. She defended her Ph.D. thesis titled “Development and Implementation of New Sensor and Biosensor Solutions Integrated into the Lab-on-Chip Concept” in 2024. Throughout her career, Dr. Kundacina has been involved in eight national and international research projects and has worked as a visiting researcher at the Technical University of Vienna, Austria, and the French National Institute for Agriculture, Food, and Environment (INRAe) in Paris, France. Her research interests include microfluidics, CFD simulations, and the development of sensing and biosensing solutions integrated into miniaturized systems.

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Ivana Kundačina

Goran Kitić works at the position of the Head of the Center for Sensing Technologies within the BioSense Institute. He received a PhD degree from the Faculty of Technical Sciences, University of Novi Sad, Serbia in 2016 on the subject of microwave soil moisture sensors. Goran Kitić developed experience in the field of different fabrication technologies (LTCC, thin&amp;thick film technology, microfabrication, PCB, wire bonding, machining, and 3D printing) and characterization methods (electron microscopy, EDS spectroscopy, UV/Vis spectroscopy, optical profilometry, electrical measurements). His research interests are related to the development of sensors applied in agriculture, food sector and environmental protection. Some of the developed sensor prototypes involve a multispectral sensor for plant status assessment, soil moisture sensor, a device for early mastitis detection, nitrates in water detector. Besides stated, he is heavily involved in the development of a robotic system for soil sampling and analysis. His research work resulted in seven international journal publications, and numerous papers published on international conferences. During his research career, he was involved in 13 international and national projects.

Goran Kitić

In 2009, Georges Dubourg received the engineer degree in electronics from the University of Sciences and Technologies in Bordeaux, France. Afterwards, he received his Ph.D degree in microelectronic from the University of Bordeaux in 2012. During his Ph.D thesis, he introduced a new activity in the IMS laboratory concerning organic MEMS for biosensing applications. From 2013 to 2014, he worked in the flied of microfluidic as a postdoctoral research fellow in the Institute of Electronics, Microelectronics and Nanotechnology (IEMN) at the University of Lille, France. Then he undertook postdoctoral research in Serbia at the University of Novi Sad before taking up his current senior researcher position at BioSense Institute in which he has launched from scratch a new research activity in the field of flexible electronic and sensors. His research interests include the design, fabrication and characterization of flexible sensors and microfluidic devices for biomedical application. From 2018, Georges is project coordinator of CISTEM project (H2020‐MSCA‐RISE‐2017) which aims to develop a new generation of organ-on-chip for modeling cardiovascular diseases.

Georges Dubourg

Mila Djisalov has a BSc in Biochemistry and a Master’s degree in Molecular Biology. She is currently a Ph.D. Student of Biochemistry at the Faculty of Sciences in Novi Sad and a Junior Researcher in BioSense Institute. Her main research interests are biosensor development and molecular tools for pathogen detection, cellular agriculture, and the development of lab-on-a-chip platforms. Mila is also one of the co-founders of the student group The BioSense Alt. Protein Project formed at the BioSense Institute.

MIla Đisalov

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Logo ENG

National

<a href="https://biosens.rs/en/themes/biosensors">Biosensors for toxin and pathogen detection</a>

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<a href="https://biosens.rs/en/themes/materials-science-nanomaterials-silica-nanomaterials-2">Water purification based on functionalized nanomaterials and hybrid membranes</a>

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<a href="https://biosens.rs/en/themes/materials-science-nanomaterials-silica-nanomaterials-2">Removal of iron from well water in irrigation systems</a>

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<a href="https://biosens.rs/en/projects/water-quality-monitoring-system-with-multi-parameter-iot-sensor-nodes-for-proactive-water-resource-management-2019-2022-e13044">Instrument for detection of pollutant and biochemical contamination of water</a>

Environmental monitoring applications

Biosensors are analytical devices for detection of the biologic material which may include biomolecules, biological structures or microorganisms (bacteria, viruses, algae, spores). Nowadays, they are being used for food safety evaluation, environmental monitoring, or for early detection of infectious diseases in biomedicine.

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We work on the development of new point-of-need biosensor transducers, biofunctionalization of the sensors with bioreceptors, and sensor integration into microfluidic systems. Developed biosensors are being implemented for detection of bacteria in food and water, detection of cyanobacterial toxins produced during harmful algal blooms, as well as for a fast detection of faecal bacteria and other microbiological pathogens in water.

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More concrete, we focus our research on development of new nanomaterials for functionalization of the biosensor electrodes and fabrication of new electrochemical transducers using different fabrication technologies. Particular attention is dedicated to new materials which will enable selective and efficient binding of the analytes and to the novel biosensor designs to increase the selectivity, sensitivity and limit of detection. Therefore, we develop the novel methods of graphene modification, and the mask-less methods of graphene patterning and integration to conventional industrial routes.

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A special focus is placed on biosensors containing enzymes, antibodies and nucleic acids (DNA, RNA) as recognition elements. This research is performed in close collaboration with the Center for Biosystems in which experts work on biomolecular engineering and molecular detection methods including the cutting-edge isothermal methods for nucleic acid amplification such as LAMP (loop-mediated isothermal amplification) and RPA (recombinase polymerase amplification) and other ways of molecular detection which can be well combined with the biosensing methods.

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In addition, BioSense Institute works on the new types of biosensors for monitoring nutrients and products of cellular metabolism during cultured meat production, as another example of excellent multidisciplinary collaboration with the Center for BioSystems and the researchers working on Cellular agriculture topics.

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<img class="alignnone wp-image-1508 " src="https://api.biosens.rs/wp-content/uploads/2021/06/58.png" alt="" width="701" height="417" />

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Collaborative projects with the Center for Biosystems:

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<a href="http://www.ipanema2020.com/">H2020 MSCA-RISE IPANEMA GA 872662</a> - Integration of PAper-based Nucleic acid testing mEthods into Microfluidic devices for improved biosensing Applications

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<a href="http://www.realsense.rs/">REALSENSE1</a> - Monitoring of cell culture parameters using sensors for biomass and nutrients/metabolites in media: Lab-on-a-Chip (LOC) approach, 2019-2021; Good Food Institute Inaugural Competitive Grant Program.

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<a href="http://www.realsense.rs/">REALSENSE2</a> – From lab-on-a-chip to custom bioreactor: scale up modeling study, 2020-2022 Good Food Institute Competitive Grant Program

Biosensors

Microfluidics deals with manipulation and control of small quantity of fluids in micro- and nano-channels and as such, it initiated revolution in different fields including microbiology, medicine, and chemical analysis.

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We focus our research on analysis and design of different microfluidic components, development of the new materials for microfluidic chips, and integration of the microfluidics with different detection systems and electronics. To that end, we employ different fabrication technologies PDMS, LTCC, 3D and inkjet printing, bio-printing, xurography, and hybrid technologies.

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Since the utilization of only one microfabrication technology cannot fulfill all the requirements set by the microfluidic chip, we also employ hybrid fabrication approach, i.e. we combine various technologies with various materials to realize microfluidic chips with high resolution, small dimension, and high flow rate. For instance, hybrid approach is used to develop multilayered filtration units, high flow rate mixers, as well as pollen separation pinch flow.

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Also, we integrate microfluidic concept and reagent storages with various transduction principles and sensors, to develop lab-on-a-chip platform which finds application in in-field environment monitoring, biomedicine and food analysis.

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Moreover, we work on development of microfluidic reactor that integrates cell culture system, different sensors for nutrient concentration and metabolic waste products measurement, optical detection system and electronics into single chip.

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Our efforts are also focused on medical applications - we combine the top notch microfluidic technologies and organ-on-a-chip concept, along with the technology of induced pluripotent stem cells, to design a new generation of heart-on-a-chip for the patient treatment.
In addition, we develop a microfluidic platform able to reproduce the dynamic and heterogeneity of the soil conditions while implementing monitoring systems for investigating the root/plant–microbiome interactions in a microfluidic device.

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<img class="alignnone wp-image-1529 size-full" src="https://api.biosens.rs/wp-content/uploads/2021/06/70.jpg" alt="" width="763" height="323" />

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PROJECTS:

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CISTEM - Heart On chip based on induced pluripotent Stem cell Technology for personalized Medicine, H2020-MSCA-RISE-2017, GA No. 778354

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REALSENSE1 - Monitoring of cell culture parameters using sensors for biomass and nutrients/metabolites in media: Lab-on-a-Chip (LOC) approach, 2019-2021; Good Food Institute Inaugural Competitive Grant Program.

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IPANEMA - Integration of PAper-based Nucleic acid testing mEthods into Microfluidic devices for improved biosensing Applications, H2020-MSCA-RISE-2019, 2020-2024. GA No. 872662,

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NANOFACTS - Networking Activities for Nanotechnology-Facilitated Cancer Theranostics, H2020 - WIDESPREAD-05-2020 – Twinning, 2021-2024. GA No. 952259

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Development and Integration of Microfluidic Biosensors for Meat Safety monitoring in farm-to-slaughterhouse continuum (DIBMES), Innovation fond, Serbia, Call: Proof of Concept ID: 5524

Microfluidics

Since carbon atomic lattice has high physical and chemical stability, carbon nanomaterials like carbon nanotubes and graphene present a promising platform for sensing of various liquid and gaseous analytes. However, high sensitivity to the changes in the environment, makes carbon nanomaterials also poorly selective to specific targets.

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In that sense, we investigate the ways of local tuning of the properties of graphene and carbon nanotubes using chemical and physical modification of their atomic lattices, to increase their sensing potential in terms of responsivity and selectivity. We are developing mask-less processes for local modification of materials properties, using various types of organic semiconductors monolayers. In addition, novel optical methods based on high energy ultraviolet or ultrafast laser are developed to alter graphene physical and chemical properties or reduction process of graphene oxide.

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Furthermore, we investigate carbon nanostructures functionalization with different materials, such as metal and metal oxides nanoparticles, (bio)polymers or semiconductor organic molecules, with the final goal to achieve the properties of carbon nanomaterials that meet requirements for high-performance sensors.

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We are particularly focused on electrochemical and chemoresistive biological sensors based on carbon nanotubes or graphene modified with specific biomolecules, for detection of small molecules, toxins, antibiotics, and pathogens in food and livestock.

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<img class="alignnone wp-image-1520 size-full" src="https://api.biosens.rs/wp-content/uploads/2021/06/65.jpg" alt="" width="604" height="461" />

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Carbon nanomaterials

MicoLabAptaSens- Microfluidic Lab-on-a-Chip platform for fast detection of pathogenic bacteria using novel electrochemical aptamer-based biosensors

Centar za senzorske tehnologije

Cilj projekta: MicroLabAptaSens namerava da napravi inovaciju u razvoju novih biosenzora za jednostavnu i brzu detekciju različitih patogena u cilju primene u bezbednosti hrane, praćenju životne sredine i zdravstvenoj zaštiti.
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O projektu: Bakterijske bolesti koje se prenose hranom predstavljaju sve veći problem javnog zdravlja na globalnom nivou i jedan od najvažnijih zahteva u bezbednosti hrane je razvoj tačnih i ranih dijagnostičkih alata za bolesti koje se prenose hranom. Projekat MicroLabAptaSens koristi komplementarno znanje i stručnost vrhunskih naučnika kako bi: 1) Sproveo najsavremenija istraživanja u multidisciplinarnoj oblasti senzora, mikrofluidike, nanomaterijala, biotehnologije i bezbednosti hrane sa glavnim ciljem da: 2) Razvije inovativne jeftine senzorske uređaje za detekciju 3 bakterije koje se najčešće prenose hranom (Listeria monocitogenes, Salmonella enteritidis i Campilobacter jejuni) koje su primenljive za upotrebu na terenu i za 3) Fabrikuju i validaciju mikrofluidičnih laboratorija na čipu testova sa integrisanim senzorom i skladištima reagensa na čipu, konačno sa ciljem 4) njihove upotrebe u poljoprivredno-prehrambenom i biotehnološkom sektoru. Novina se oslanja na novu klasu biosenzora zasnovanih na nanomaterijalima funkcionalizovanih na aptamerima pogodnih za integraciju u jedinstvene jeftine laboratorija na čipu testove. Primena naprednih mikro- i nano-tehnologija će omogućiti integraciju sa reagensima za analizu više patogena u brze testove prilagođene korisniku koji neće zahtevati skupu laboratorijsku opremu, obučeno osoblje i posebne uslove. Takvi testovi ispuniće sve ASSURED (pristupačne, osetljive, specifične, prilagođene korisniku, brze i robusne, bez opreme i isporučiv krajnjim korisnicima) kriterijume koje preporučuje Svetska zdravstvena organizacija. Za obradu i vizuelizaciju podataka biće razvijen elektronski modul za očitavanje i aplikacija za pametne telefone. Konačni cilj je ekonomičan senzorski uređaj koji može da zadovolji potrebe tržišta i MF testove za aplikacije u zdravstvu, praćenju životne sredine i sektorima bezbednosti hrane i koji se lako može proširiti na masovnu proizvodnju ili prilagoditi za druge specifične primene kao što je detekcija virusa ili otkrivanje patogena u vazduhu.
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Naučni savetnik u oblasti tehničko–tehnoloških nauka, telekomunikacija i informacionih tehnologija

Ivana Milošević je student doktorskih studija na studijskom programu Biotehnologija na Tehnološkom fakultetu, Univerziteta u Novom Sadu. Od januara 2023. godine, Ivana je zaposlena kao istraživač-pripravnik na Institutu BioSens. U Centru za senzorske tehnologije, Ivana se specijalizuje za razvoj biosenzora na bazi flagelarnih filamenata. Njen rad se fokusira na povećanje osetljivosti ovih biosenzora za detekciju teških metala, posebno nikla (Ni). Ivanin doprinos se proteže na modifikaciju elektrohemijskih biosenzora, pokazujući njenu posvećenost unapređenju biotehnoloških inovacija.

Goran Kitić je zaposlen na poziciji Rukovodioca centra za senzorske tehnologije na Institutu BioSens. Doktorirao je na Fakultetu tehničkih nauka, na Univerzitetu u Novom Sadu 2016. godine na temu mikrotalasnih senzora vlažnosti zemljišta. Goran Kitić ima iskustva u oblastima raznih tehnologija fabrikacije (LTCC, debeloslojna i tankoslojna tehnologija, mikrofabrikacija, PCB, wire bonding, mašinska obrada i 3D štampa) i metoda karakterizacije (elektronska mikroskopija, EDS spektoroskopija, UV/Vis spektroskopija, optička profilometrija, električna merenja). Njegova istraživanja su u vezi sa razvojem senzora koji svoju primenu imaju u poljoprivredi, prehrambenom sektoru i zaštiti životne sredine. Neki od razvijenih prototipa senzora uključuju multispektralni senzor za utvrđivanje statusa biljaka, senzor vlažnosti zemljišta, uređaj za rano otkrivanje mastitisa, detektor nitrata u vodi. Pored navedenog, u velikoj meri je uključen u razvoj robotskog sistema za uzorkovanje i analizu zemljišta. Njegovo istraživanje je publikovano u 7 međunarodnih časopisa, i brojnim radovima objavljenim na međunarodnim konferencijama. Tokom svoje karijere, učestvovao je u 13 međunarodnih i nacionalnih projekata.

Dr Radonić je doktorirao iz oblasti elektronike 2010. godine na Univerzitetu u Novom Sadu. Autor je 2 poglavlja u knjizi, 30 radova u naučnim časopisima, više od 60 konferencijskih radova, 10 tehničkih rešenja i ima više od 440 citata. Dr Radonić je bio rukovodilac na četiri nacionalna projekta, jednom projektu koji je finansirala Evropska banka za istraživanje i razvoj, jednom bilateralnom projektu između Srbije i Slovenije, projektu koji je finansirao Good Food Institute iz SAD-a i vođa radnog paketa u jednom FP7 projektu – Innosens i jednom H2020 – Antares. Učestvovao je u brojnim istraživačkim projektima iz programa FP6, FP7, H2020, COST, Eureka, IPA. Član je grupa IEEE i MAGEO (Mikrotalasi u poljoprivredi, životnoj sredini i posmatranju Zemlje) i član tehničkih programskih odbora konferencije EUROCON (Computer as a Tool, 2015), Salamanka, Španija i Sensodevice konferencije 2019, Nica, Francuska. Član upravnog odbora Instituta BioSense od 2015-2020 i potpredsednik Naučnog veća Instituta BioSense 2015-2020. Od 2022 obavlja funkciju pomoćnika direktora za nauku. Recenzent u mnogim naučnim časopisima: Scientific Reports, Lab-on-Chip, IEEE Sensors Journal, MDPI Sensors, MDPI Electronics, Sensors and Actuators B, IEEE Wireless and component letters, Electronic letters, etc. Oblast naučnog istraživanja dr Radonića je mikrofluidika, razvoj senzora (mikrotalasni, optički, mikrofluidni senzori i biosenzori), elektronskih, RF i mikrotalasnih pasivnih komponenti (filteri, spojnica, diplekser itd.), antena i RFID sistema, periodični i veštački materijali, tehnologije fabrikacije, merenja i karakterizacija.

Stefan Jarić je zaposlen u Centru za senzorske tehnologije kao istraživač-saradnik i student završne godine doktorskih studija fizike materijala na Departmanu za fiziku, Prirodno-matematičkog fakulteta u Novom Sadu. Stefan se bavi razvojem i istraživanjem novih rešenja na polju biosenzora zasnovanih na grafenskim tranzistorima sa efektom polja i specifičnih sintetičkih receptora (aptamera) za detekciju raznih analita iz sfere zaštite životne sredine (toksini) i medicine (biomarkeri). Njegova ekpertiza se razvija u pravcu mikro i nanofabrikacije senzorskih platformi (fotolitografija, fizičko naparavanje materijala), karakterizacije materijala (Ramanova spektroskopija, mikroskopija atomskih sila) i električnih karakteristika biosenzora.

Slavica Savić je diplomirala 2002 godine na Fakultetu za fizičku hemiju, Univerzitet u Beogradu. Zvanje doktora nauka u oblasti materijala u elektronici je stekla 2009 godine na Fakultetu tehničkih nauka Univerziteta u Novom Sadu. Od januara 2003. do septembra 2008. radila je na Institutu tehničkih nauka SANU kao istraživač saradnik. Od septembra 2008. do jula 2015. bila je zaposlena kao istraživač saradnik, naučni saradnik i viši naučni saradnik na Odseku za materijale Instituta za multidisciplinarna istraživanja Univerziteta u Beogradu. Od jula 2012. do decembra 2012. bila je gostujući istraživač (istraživač na postdoktorskim studijama) na Pontificia Universidade Catolica (PUC), Rio de Janeiro, Brazil. Od jula 2015.do jula 2020. radila je kao viši naučni saradnik na Institutu BioSens. Od jula 2020. radi kao naučni savetnik u Centru za senzorske tehnologije Instituta BioSens. Učestvovala je na više nacionalnih i međunarodnih projekata a rukovodila je bilateralnim istraživačkim projektom sa Italijom kao i projektom finansiranim od Evropske banke za istraživanje i razvoj.Oblasti njenog istraživanja su nauka o materijalima, procesiranje keramičkih materijala i njihova karakterizacija, fizika i hemija čvrstog stanja. Specifična oblast istraživanja: Sinteza i karakterizacija dopirane BCY10 keramike primenjene kao elektrolita za srednjetemperaturne gorivne ćelije; sinteza i karakterizacija termistorskog materijala nikl manganita; Termoelektrični materijali: mehanohemijski podržana sinteza u čvrstom stanju kao i citratna gel sinteza čistog natrijum kobaltita kao i dopiranog bakrom. Trenutne istraživačke aktivnosti su usmerene ka sintezi i karakterizaciji metal oksidnih materijala MOx (M=Ti, Sn, Zn) sa primenom u gasnim senzorima i senzorima vlage kao i 2D materijalima kao gasnim senzorima za primenu u poljoprivredi, kao i medicinskim uređajima za analizu daha itd. Značaj njenih istraživanja je potvrđen sa više od 50 naučnih radova objavljenim u visoko rangiranim međunarodnim časopisima.

Dr Nikolina Janković je stariji Istraživač u Centru za senzorske tehnologije Instituta BioSens. Autor i koautor više od 40 radova u časopisima i na konferencijama, kao i 5 poglavlja u knjigama. Njena polja interesovanja uključuju metamaterijale, akustiku, plazmoniku, senzore i mikrotalasna pasivna kola. Koordinator je H2020 MSCA-RISE projekta NOCTURNO i EUREKA projekat WaQUMoS u Srbiji. In poslednjih sedam godina učestvovala je u 2 FP7 projekta MultiWaveS i InnoSense (menadžer projekta i vođa radnog paketa), kao i u 3 H2020 projekta (H2020 Teaming ANTARES (vođa radnog paketa), NOCTURNO (rukovodilac) i SHealthy).

Dr Marko Radović je diplomirao 2005. na Fizičkom Fakultetu pri Beogradskom univerzitetu. 2008. je odbranio magistarski rad na Fizičkom Fakultetu na Univerzitetu u Beogradu. Doktorsku disertaciju je odbranio 2015. na Fizičkom Fakultetu u Beogradu. Od aprila 2006. je zaposlen na Institutu za Fiziku u Beogradu. Od februara 2015. je zaposlen kao naučni saradnik na Biosens Institutu. Naučno istraživačka delatnost Dr Radovića je fokusirana na tzv. “bottom-up approach” gde su povezane sinteza nanomaterijala, karakterizacija I funkcionalizacija nanomaterijala u dizajnu senzorskih optoelektronskih i fotokatalitičkih uređaja. Tokom dosadašnje karijere publikovao je više od 25 naučnih radova u međunarodnim i domaćim časopisima.

Dr Ivana Kundačina je naučni saradnik na Institutu Biosens. Završila je osnovne i master studije fizike, kao i doktorske studije primenjene fizike na Prirodno-matematičkom fakultetu, Univerziteta u Novom Sadu. Doktorsku disertaciju pod nazivom „Razvoj i implementacija novih senzorskih i biosenzorskih rešenja primenom koncepta Laboratorija-na-čipu“ odbranila je 2024. godine. Tokom svoje karijere, Ivana Kundačina je učestvovala u osam nacionalnih i međunarodnih istraživačkih projekata i radila kao gostujući istraživač na Tehničkom univerzitetu u Beču, Austrija, i institutu INRAe u Parizu, Francuska. Njene oblasti istraživanja uključuju mikrofluidiku, CFD simulacije, kao i razvoj senzorskih i biosenzorskih rešenja integrisanih u minijaturizovane sisteme.

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Georges Dubourg је zaposlen u Institutu BioSens од 16. oktobra 2015. godine. Prethodno je bio zaposlen na Fakultetu tehničkih nauka, na Departmanu za energetiku, elektroniku i telekomunikacije, od septembra 2014. godine kao saradnik na FP7 projektu InnoSense. Od januara 2013. do jula 2014. godine radio je kao posle doktorski istaživač na Institutu za elektroniku, mikro i nanoelektroniku (IEMN) na Univerzitetu u Lilu (Francuska). Doktorske studije završio je na Univerzitetu u Bordou (Francuska), gde je radio kao istraćivač u laboratoriji Integracija: od Materijala do Sistema (IMS). U toku svog dosadašnjeg rada, dr Georges Dubourg se prvenstveno bavio naučnim istaživanjima u oblasti mikroelektronike i mikrofabrikacionih tehnologija, gde je ostvario zapažene rezultate na međunarodnom nivou. Takođe, učestvovao je u izvođenju auditornih i laboratorijskih vežbi kao asistent u nastavi od 2010. do 2012. na Univerzitetu u Bordou (Francuska) na nekoliko predmeta iz oblasti elektronike i tehnologija mikrofabrikacije na osnovnim akademskim i master studijama. Sarađivao je sa studentima pri izradi obaveznih projekata, diplomskih i master završnih radova. Domen istraživanja obuhvataju razvoj i fabrikaciju fleksibilnih senzora, mikrofluidiku i razvoj platformi organ-na-čipu. Georges je koordinator Horizon2020 projekta CISTEM-GA 778354.

Mila Đisalov je diplomirani biohemičar i master molekularne biologije. Trenutno je student doktorskih studija biohemije na Prirodno-matematičkom fakultetu u Novom Sadu i istraživač-pripravnik na Institutu BioSens. Njena glavna istraživačka interesovanja vezana su za razvoj biosenzora i molekularnih alata za detekciju patogena, ćelijska poljoprivreda i razvoj laboratorija na čipu. Mila je takođe jedan od osnivača studentske grupe The BioSense Alt. Protein Project, osnovane na Institutu BioSens.

Mila Đisalov

Fond za nauku logo za oba projekta

Nacionalni

<a href="https://biosens.rs/themes/biosenzori">Biosenzori za detekciju toksina i patogena</a>

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<a href="https://biosens.rs/themes/silikatni-nanomaterijali-3">Prečišćavanje vode korišćenjem funkcionalizovanih nanomaterijala i hibridnih membrana</a>

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<a href="https://biosens.rs/themes/silikatni-nanomaterijali-3">Uklanjanje gvožđa iz bunarske vode u sistemima za navodnjavanje</a>

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<a href="https://biosens.rs/projects/remote-sensing">Instrument za detekciju polutanata i biohemijske kontaminacije vode</a>

Primene u monitoringu životne sredine

Biosenzori su analitički uređaji koji služe za detekciju biološkog materijala kao što su biomolekuli, biološke strukture ili mikroorganizmi (bakterije, virusi, alge, spore). Široku primenu biosenzori nalaze u kontroli bezbednosti hrane, monitoringu životne sredine, kao i u ranom otkrivanju infektivnih bolesti u biomedicini.

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Naša istraživanja su usmerena na razvoj novih biosenzorskih transdjusera, biofunkcionalizaciju senzora sa bioreceptorima, i integraciju senzora u mikrofluidične sisteme. Razvijeni senzori se koriste za detekciju bakterija u hrani i vodi, za detekciju cijano toksina nastalih tokom cvetanja algi, kao i za brzu detekciju fekalnih bakterija i drugih mikrobioloških patogena u vodi.

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Bavimo se i razvojem novih nanomaterijala za funkcionalizaciju biosenzorskih elektroda i fabrikaciju novih elektrohemijskih biosenzora koristeći različite tehnologije fabrikacije. Deo istraživanja je usmeren na nove materijale koji će omogućiti selektivno i efikasno vezivanje analita, kao i na projektovanje biosenzora u cilju poboljšanja njihove selektivnosti, osetljivosti i limita detekcije. Bavimo se i novim metodama modifikacije grafena, novim metodama za njegovu sintezu i integraciju u konvencionalne industrijske procese.

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Posebna pažnja je posvećena biosenzorima na bazi enzima, antitela i nukleinskih kiselina (DNK, RNK). Ova istraživanja se izvode u bliskoj saradnji sa Centrom za biosisteme u kojem istraživači rade na metodama biomolekularnog inženjerstva i molekularne dijagnostike što uključuje i najsavremenije izotermalne metode za umnožavanje nukleinskih kiselina kao što su LAMP (Loop Mediated Isothermal Amplification) i RPA (Recombinase Polymerase Amplification) koje se mogu dobro kombinovati sa biosenzorima razvijenim u okviru Centra za senzorske tehnologije.

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Takođe, Institut BioSens radi na novim vrstama biosenzora za monitoring hranljivih materija i produkata metabolizma ćelija u procesu proizvodnje kultivisanog mesa, što je još jedan primer dobre multidisciplinarne saradnje sa Centrom za biosisteme i istraživačima koji rade u okviru oblasti ćelijske poljoprivrede.

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<img class="alignnone wp-image-1508 " src="https://api.biosens.rs/wp-content/uploads/2021/06/58.png" alt="" width="701" height="417" />

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Zajednički projekti sa Centrom za biosisteme:

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H2020 MSCA-RISE IPANEMA GA 872662 - Integration of PAper-based Nucleic acid testing mEthods into Microfluidic devices for improved biosensing Applications

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REALSENSE1 - Monitoring of cell culture parameters using sensors for biomass and nutrients/metabolites in media: Lab-on-a-Chip (LOC) approach, 2019-2021; Good Food Institute Inaugural Competitive Grant Program.

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REALSENSE2 – From lab-on-a-chip to custom bioreactor: scale up modeling study, 2020-2022 Good Food Institute Competitive Grant Program

Biosenzori

S obzirom na to da atomska rešetka ugljenika poseduje visoku fizičku i hemijsku stabilnost, ugljenični nanomaterijali, kao što su ugljenične nanocevi i grafen, predstavljaju obećavajuću platformu za detekciju različitih tečnih ili gasnih analita. Međutim, visoka osetljivost na promene u okolini čini ugljenične nanomaterijale slabo selektivnim prema specifičnim analitima.

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U tom smislu, istražujemo mogućnosti lokalnog podešavanja svojstava grafena i ugljeničnih nanocevi pomoću fizičkih i hemijskih modifikacija njihove atomske rešetke, kako bismo povećali njihov potencijal detekcije u pogledu responzivnosti i selektivnosti. Razvijamo procese bez maske za finu modifikaciju svojstava materijala korišćenjem različitih tipova organskih poluprovodnih monoslojeva. Pored toga, razvijaju se nove optičke metode zasnovane na UV ili ultra brzim laserima visoke energije za promenu fizičkih i hemijskih osobina grafena ili za redukciju grafen-oksida.

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Osim toga, ispitujemo mogućnost funkcionalizacije ugljeničnih nanostruktura različitim materijalima, kao što su nanočestice metala i njihovih oksida, (bio)polimeri ili poluprovodni organski molekuli, sa ciljem dobijanja ugljeničnih nanomaterijala koji zadovoljavaju zahteve senzora visokih preformansi.

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Poseban fokus jeste razvoj elektrohemijskih i hemorezistentnih bioloških senzora na bazi ugljeničnih nanocevi ili grafena modifikovanih specifičnim biomolekulima, za detekciju malih molekula, toksina, antibiotika i patogena u hrani i životinjama.

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<img class="alignnone wp-image-1520 size-full" src="https://api.biosens.rs/wp-content/uploads/2021/06/65.jpg" alt="" width="604" height="461" />

Ugljenični nanomaterijali

Mikrofluidika je oblast koja se bavi manipulacijom i kontrolom malih količina fluida u mikro- i nano-kanalima i kao takva mikrofludika je napravila pravu malu revoluciju u mikrobiološkim, medincinskim i hemijskim analizama.

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Naša istraživanja su fokusirana na analizu i projektovanje različitih mikrofludičinih komponenta, razvoj novih materijala za mikrofluidične čipove i integraciju mikrofluidike sa različitim sistemima za detekciju i elektronikom. U tu svrhu koristimo različite tehnologije fabrikacije, kao što su Polidimetilsiloksan (PDMS), niskotemperaturna zajedno pečena keramika (LTCC), 3D, inkdžet i bio-štampa, ksirografija, kao i hibridne tehnologije.
Kako korišćenje samo jedne tehnologije fabrikacije ne može da ispuni sve zahteve savremenih mikrofluidičnih sistema, koristimo hibridni pristup - kombinujemo različite tehnologije sa različitim materijalima da bismo dobili mikrofluidične čipove sa visokom rezolucijom, malim dimenzijama i visokim protocima. Na primer, hibridni pristup se koristi za razvoj višeslojnih čipova za filtraciju, mešača fluida za visoke protoke, kao i separatora za polen.

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Takođe, integrišemo mikrofluidne čipove sa reagentima za analizu na čipu sa različitim tipovima senzora u cilju razvoja labratorije na čipu za detekciju parametara fluida, koji nalaze primenu u kontroli mešanja fluida, biomedicine, monitoringu ekosistema i analizi tečne hrane.
Osim toga, radimo na razvoju mikrofluidičnog reaktora, koji integriše sistem za ćelijsku kulturu, različite senzore za detekciju koncentracije hranljivih materija i merenje metaboličkih otpadnih proizvoda, optičku detekciju i elektroniku u jedan jedinstveni sistem na čipu.

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Prateći ovaj koncept, razvijamo i mikrofluidičnu platformu koja ima mogućnost da reprodukuje dinamiku i heterogenost zemljišta na taj način razvijajući sistem za monitoring interakcije između biljke i mikrobioma.

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<img class="alignnone wp-image-1529 size-full" src="https://api.biosens.rs/wp-content/uploads/2021/06/70.jpg" alt="" width="763" height="323" />

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PROJEKTI:

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CISTEM - Heart On chip based on induced pluripotent Stem cell Technology for personalized Medicine, H2020-MSCA-RISE-2017, GA No. 778354

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REALSENSE1 - Monitoring of cell culture parameters using sensors for biomass and nutrients/metabolites in media: Lab-on-a-Chip (LOC) approach, 2019-2021; Good Food Institute Inaugural Competitive Grant Program.

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IPANEMA - Integration of PAper-based Nucleic acid testing mEthods into Microfluidic devices for improved biosensing Applications, H2020-MSCA-RISE-2019, 2020-2024. GA No. 872662,

MicoLabAptaSens- Microfluidic Lab-on-a-Chip platform for fast detection of pathogenic bacteria using novel electrochemical aptamer-based biosensors

Themes:

People:

Centers:

CST