Center for Biosystems

Center for sensing technologies

Project aim: IPANEMA has formed a large network of multidisciplinary expertise to rapidly realise the potential of paper-based microfluidic platforms.
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About the project: Nucleic acids (including deoxyribonucleic acid or DNA and ribonucleic acid or RNA) are found in all cells and viruses. Nucleic acid amplification tests (such as PCR tests) are increasingly used to facilitate detection of the DNA or RNA associated with bacteria, viruses, or other pathogens even when present in very small quantities. However, they are still relatively complex and expensive for widespread use in point-of-care (POC) rapid diagnostic systems. With partners in Europe, USA, China, Russia, and Israel that include SMEs and academics, IPANEMA is working on several different technologies that can be integrated with smart phones for sophisticated yet cost-effective and easy-to-use POC diagnostics.
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Senior research associate in the field of natural/mathematical sciences - biology

Ivana Gađanski

Dr Radonic received a PhD in electronics in 2010 at University of Novi Sad. He authored and co-authored 2 book chapters, 30 journal papers, more than 60 conference papers, 10 technical solutions, and has more than 440 citations. Dr Radonić was the project leader in four Serbian national projects, one project financed by European Bank for Research and Development, one Bilateral project between Serbia and Slovenia, project financed by Good Food Institute, USA and WP leader in one FP7 project–InnoSense and one H2020 project–ANTARES. He participates in a number of research FP6, FP7, H2020, COST, EUREKA, IPA projects. He is a member of IEEE and MAGEO (Microwaves in Agriculture, Environment and Earth Observation) groups and was a member of Technical Program Committees of EUROCON (Computer as Tool, 2015) conference, Salamanca, Spain and Sensordevices conference 2019, Nice, France. Steering committee member of the BioSense Institute 2015-2020, and the vice president of Scientific council of the BioSense Institute 2015-2020. From 2022, he is the assistant director for science. Reviewer in different scientific journals: Scientific Reports, Lab-on-Chip, IEEE Sensors Journal, MDPI Sensors, MDPI Electronics, Sensors and Actuators B, IEEE Wireless and component letters, Electronic letters, etc. The main expertise of Dr Radonic is microfluidics, sensors development (microwave, optical, microfluidic sensors and biosensors), electronic, RF and microwave passive components (filters, coupler, diplexer, etc.), antennas and RFID systems, periodical and artificial materials, fabrication technologies, measurements and characterization.

Vasa Radonić

Stefan Jarić is employed at the Center for Sensing Technologies as a Research Assistant and a final-year PhD student in materials physics at the Department of Physics, Faculty of Sciences at the University of Novi Sad. Stefan works on the development and research of new solutions in the field of biosensors based on graphene field effect transistors and specific synthetic receptors (aptamers) for the detection of various analytes in the area of environmental protection (toxins) and medicine (biomarkers). His expertise is developing in the direction of micro and nanofabrication of sensor platforms (photolithography, physical vapor deposition of materials), characterization of materials (Raman spectroscopy, atomic force microscopy) and electrical characteristics of biosensors.

Stefan Jarić

Slobodan Birgermajer is research associate in the Center for Sensing Technologies at the BioSense Institute. Mr. Birgermajer received his PhD in microware electronics form the Faculty of Technical Sciences, University of Novi Sad, in 2019. He is currently working on development and application of the sensors in agriculture, food industry, and environmental monitoring, and he is actively involved in many H2020 projects such as ANTARES, IPANEMA SHealty, etc. His main research interest includes microwave passive devices, photonic crystals, microfluidic sensors, and 3D and 4D printing.

Slobodan Birgermajer

Dr. Nikola Knežević is a Full research professor at the Center for Sensor Technologies at the Biosense Institute. He received his doctorate in 2009 at Iowa State University, USA, in synthesizing and functionalizing porous silica nanoparticles for stimuli-responsive and targeted drug delivery. He gained further research experience as a postdoctoral fellow at the University of Houston (research in the field of multistep organic synthesis), Universidad Complutense de Madrid (nanomaterials science), Institute Charles Gerhardt Montpellier (nanomaterials science), FP7 - ERA ERA Chairs postdoctoral fellow at the Vinča Nuclear Institute and as Pole Chimie Ballard Visiting Professor at the University of Montpellier. Nikola is the author/co-author of over 40 scientific publications, including 2 book chapters and one patent, with over 1900 citations and an H index of 21. Nikola is the coordinator of one H2020 project (NANOFACTS GA 952259), three Serbian national projects (PRECAST – 6060755, proof of concept – 5566, technology transfer -1135), and two bilateral research projects (with France and Germany). Research interests: Synthesis of functional bioresponsive nanomaterials for use in health protection and construction of biosensors.

Nikola Knežević

Nejra Omerović is a Research Assistant at the BioSense Institute. She received her BSc and MSc degree in 2015 and 2017, respectively, from the Faculty of Technology, University of Novi Sad, Serbia, where she is currently a PhD student at the Department of Materials engineering. From October 2015 to September 2016 she did an internship at AGFA-Gevaert NV, Mortsel, Belgium, where she worked at the Inkjet Ink Development Department. Her current research activities are focused on biopolymers and their nanocomposites.

Nejra Omerović

Minja Mladenović is a Junior Research Assistant at the BioSense Institute and a Ph.D. student of Chemistry at the Faculty of Sciences, University of Novi Sad. She received her Bachelor (2015) and Master (2016) degrees from the Department of Chemistry, Biochemistry and Environmental Protection at the Faculty of Sciences, as well. Her research interests include synthesis, functionalization and characterization of silica-based nanomaterials and their application in stimuli-responsive and controlled drug/biomolecules delivery.

Minja Mladenović

Dr Marko Radović graduated in 2005 from the Faculty of Physics, University of Belgrade. In 2008 he received a MSc degree at the Faculty of Physics, University of Belgrade. He received PhD title in 2015 also at Faculty of Physics, University of Belgrade. From April 2006. to February of 2017. He was employed at the Institute of Physics in Belgrade. Since February 2017. He is employed as a senior researcher at BioSense Institute. Dr Radović research interests are focused on a bottom-up approach, involving synthesis, characterization and functionalization of nanomaterials to design of gas monitoring platforms, biosensors, optoelectronic and photocatalytic devices. He has published 25 scientific papers in international and domestic journals and he is a member of the Serbian Society for Ceramic Materials.

Marko Radović

Jovana Stanojev is a research assistant and a PhD student at the Faculty of Technology, University of Novi Sad. She studied materials engineering. She is working towards finishing her PhD thesis. Topic of her PhD thesis is "Development of uncooled infrared detector based on lead selenide". Her current research focuses are development of new materials for sensors, nanomaterials and nanomembranes, microfluidics, development of organ-on-a-chip platforms, cell culturing.

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Jovana Stanojev

Ivana Podunavac is a Junior Researcher at BioSense Institute and PhD student of Applied Physics at the Faculty of Sciences, University of Novi Sad, Serbia. Her fields of research are microfluidics,
simulations, fabrication and testing of sensing devices.

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Ivana Podunavac

Mila Djisalov has a BSc in Biochemistry and a Master’s degree in Molecular Biology. She is currently a Ph.D. Student of Biochemistry at the Faculty of Sciences in Novi Sad and a Junior Researcher in BioSense Institute. Her main research interests are biosensor development and molecular tools for pathogen detection, cellular agriculture, and the development of lab-on-a-chip platforms. Mila is also one of the co-founders of the student group The BioSense Alt. Protein Project formed at the BioSense Institute.

MIla Đisalov

Ljiljana Janjusevic PhD is Research Associate at the BioSens Institute, University of Novi Sad, Serbia. Bechelor, master and doctoral studies were completed at the Department of Biology and Ecology, Faculty of Sciences, University of Novi Sad. During the doctoral studies, the focus of the research was on examining the biological activities and chemical composition of macrofungi as well as the essntial oils of some plants. Research at the Institute focuses on the development of sensors and molecular techniques for pathogen detection, biomolecular engineering and cell agriculture.

Ljiljana Janjušević

Ivana Gadjanski PhD is Research Associate Professor at BioSense Institute, University of Novi Sad, Serbia. She graduated from the Faculty of Biology, University of Belgrade as the 1st in the class (2003). She obtained PhD in Neuroscience (summa cum laude -oral presentation) from Georg-August University &amp; Max Planck Institute for Biophysical Chemistry, Goettingen, Germany (2007) and worked as a postdoctorate researcher at Ludwig-Maximillian University, Munich, Germany. Ivana then turned to the bioengineering field and spent a year as a Fulbright Visiting Scholar at Columbia University, USA - Lab for Stem cells and Tissue Engineering (Head: prof. Gordana Vunjak-Novakovic). In BioSense, she is moving further into the cellular agriculture field - developing solutions for cultivated meat. She also works on biosensing for agrifood, biomedicine and environmental monitoring. Ivana is a grantee of the Good Food Institute and coordinator of Horizon2020 projects DRAGON GA 810755 and IPANEMA GA 872662.

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Ipanema Logo

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This project has received funding from the European Union’s Horizon 2020 research and innovation program under grant agreement No. 872662. 
                        

Horizon 2020

Due to their small diameter (ca. 100 nm) and highly porous nature, mesoporous silica nanoparticles (MSNs) have high specific surface area (ca. 1000 m2/g) which can be utilized for a plethora of functionalizations, loading/adsorption of different molecules and their removal, or as a platform for signaling molecules to detect different analytes.

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Mesoporous organosilicate nanomaterial (also termed Periodic Mesoporous Organosilica (PMO)) contains a large percentage of organic groups within the nanosphere, exhibiting unique characteristics for different applications.

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Our research is being conducted toward design of new series of MSN and PMO nanomaterials, as well as their core/shell analogs.

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Surface of these nanomaterials is being modified with different molecules to specifically interact with desired tissues, pollutants, or analytes, for their efficient application in monitoring and protection of health, food and the environment.

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One of the research goals is to develop smart nanomaterials with stimuli-responsive release of MSN/PMO-loaded molecules for diagnostics, imaging, and therapy of cancer tissues.

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Polymer-MSN composite materials are also being developed for applications as antimicrobial smart food packaging and medical implants.

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Finally, PMO nanoparticles are being constructed for efficient UVA and UVB blocking and application in a safer generation of sunscreens.

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<img class="alignnone wp-image-1626 size-full" src="https://api.biosens.rs/wp-content/uploads/2021/06/slika-1.png" alt="" width="837" height="466" />

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Projects:

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PRECAST: Prediction of Cancer Treatment Effectiveness with Stimuli-responsive Nanomaterials, Science Fund of the Republic of Serbia

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<a href="https://cordis.europa.eu/project/id/952259">NANOFACTS: Networking Activities for Nanotechnology-Facilitated Cancer Theranostics H2020-WIDESPREAD-2020-5 </a>

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Novel Active Components for Health- and Environment-friendly UV-Blocking Based on Enriched Organosilica Nanomaterials, Project ID: 5566, The Innovation Fund of the Republic of Serbia

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III 44006, Ministry of Education, Science, and Technological Development of the Republic of Serbia.

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Silica nanomaterials

Biosensors are analytical devices for detection of the biologic material which may include biomolecules, biological structures or microorganisms (bacteria, viruses, algae, spores). Nowadays, they are being used for food safety evaluation, environmental monitoring, or for early detection of infectious diseases in biomedicine.

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We work on the development of new point-of-need biosensor transducers, biofunctionalization of the sensors with bioreceptors, and sensor integration into microfluidic systems. Developed biosensors are being implemented for detection of bacteria in food and water, detection of cyanobacterial toxins produced during harmful algal blooms, as well as for a fast detection of faecal bacteria and other microbiological pathogens in water.

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More concrete, we focus our research on development of new nanomaterials for functionalization of the biosensor electrodes and fabrication of new electrochemical transducers using different fabrication technologies. Particular attention is dedicated to new materials which will enable selective and efficient binding of the analytes and to the novel biosensor designs to increase the selectivity, sensitivity and limit of detection. Therefore, we develop the novel methods of graphene modification, and the mask-less methods of graphene patterning and integration to conventional industrial routes.

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A special focus is placed on biosensors containing enzymes, antibodies and nucleic acids (DNA, RNA) as recognition elements. This research is performed in close collaboration with the Center for Biosystems in which experts work on biomolecular engineering and molecular detection methods including the cutting-edge isothermal methods for nucleic acid amplification such as LAMP (loop-mediated isothermal amplification) and RPA (recombinase polymerase amplification) and other ways of molecular detection which can be well combined with the biosensing methods.

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In addition, BioSense Institute works on the new types of biosensors for monitoring nutrients and products of cellular metabolism during cultured meat production, as another example of excellent multidisciplinary collaboration with the Center for BioSystems and the researchers working on Cellular agriculture topics.

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<img class="alignnone wp-image-1508 " src="https://api.biosens.rs/wp-content/uploads/2021/06/58.png" alt="" width="701" height="417" />

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Collaborative projects with the Center for Biosystems:

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<a href="http://www.ipanema2020.com/">H2020 MSCA-RISE IPANEMA GA 872662</a> - Integration of PAper-based Nucleic acid testing mEthods into Microfluidic devices for improved biosensing Applications

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<a href="http://www.realsense.rs/">REALSENSE1</a> - Monitoring of cell culture parameters using sensors for biomass and nutrients/metabolites in media: Lab-on-a-Chip (LOC) approach, 2019-2021; Good Food Institute Inaugural Competitive Grant Program.

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<a href="http://www.realsense.rs/">REALSENSE2</a> – From lab-on-a-chip to custom bioreactor: scale up modeling study, 2020-2022 Good Food Institute Competitive Grant Program

Biosensors

Microfluidics deals with manipulation and control of small quantity of fluids in micro- and nano-channels and as such, it initiated revolution in different fields including microbiology, medicine, and chemical analysis.

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We focus our research on analysis and design of different microfluidic components, development of the new materials for microfluidic chips, and integration of the microfluidics with different detection systems and electronics. To that end, we employ different fabrication technologies PDMS, LTCC, 3D and inkjet printing, bio-printing, xurography, and hybrid technologies.

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Since the utilization of only one microfabrication technology cannot fulfill all the requirements set by the microfluidic chip, we also employ hybrid fabrication approach, i.e. we combine various technologies with various materials to realize microfluidic chips with high resolution, small dimension, and high flow rate. For instance, hybrid approach is used to develop multilayered filtration units, high flow rate mixers, as well as pollen separation pinch flow.

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Also, we integrate microfluidic concept and reagent storages with various transduction principles and sensors, to develop lab-on-a-chip platform which finds application in in-field environment monitoring, biomedicine and food analysis.

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Moreover, we work on development of microfluidic reactor that integrates cell culture system, different sensors for nutrient concentration and metabolic waste products measurement, optical detection system and electronics into single chip.

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Our efforts are also focused on medical applications - we combine the top notch microfluidic technologies and organ-on-a-chip concept, along with the technology of induced pluripotent stem cells, to design a new generation of heart-on-a-chip for the patient treatment.
In addition, we develop a microfluidic platform able to reproduce the dynamic and heterogeneity of the soil conditions while implementing monitoring systems for investigating the root/plant–microbiome interactions in a microfluidic device.

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<img class="alignnone wp-image-1529 size-full" src="https://api.biosens.rs/wp-content/uploads/2021/06/70.jpg" alt="" width="763" height="323" />

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PROJECTS:

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CISTEM - Heart On chip based on induced pluripotent Stem cell Technology for personalized Medicine, H2020-MSCA-RISE-2017, GA No. 778354

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REALSENSE1 - Monitoring of cell culture parameters using sensors for biomass and nutrients/metabolites in media: Lab-on-a-Chip (LOC) approach, 2019-2021; Good Food Institute Inaugural Competitive Grant Program.

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IPANEMA - Integration of PAper-based Nucleic acid testing mEthods into Microfluidic devices for improved biosensing Applications, H2020-MSCA-RISE-2019, 2020-2024. GA No. 872662,

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NANOFACTS - Networking Activities for Nanotechnology-Facilitated Cancer Theranostics, H2020 - WIDESPREAD-05-2020 – Twinning, 2021-2024. GA No. 952259

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Development and Integration of Microfluidic Biosensors for Meat Safety monitoring in farm-to-slaughterhouse continuum (DIBMES), Innovation fond, Serbia, Call: Proof of Concept ID: 5524

Microfluidics

Biomolecules, including DNA, RNA, proteins, peptides and the constellation of small molecules made by cells, are the molecules that fuel life. Biomolecular engineering (BME) is the analysis and engineering of these living systems. BME research uses techniques from synthetic biology, systems biology, biomedical research and biotechnology, biochemistry and biophysics of biological systems and cell biology to quantify and manipulate the structure and function of molecules and macromolecular assemblies for potential applications in:

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· Biotechnology: Biosensing and bioanalytical devices, microfluidics-enabled cell culture technologies, (e.g. organ-on-a-chip), development and application of novel molecular tools (applicable for food safety evaluation)

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· Fundamental processes of living systems: host pathogen interactions, cellular and subcellular organization, regulation and control of biological networks

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· Human and animal disease: design and engineering of diagnostic methodologies, cell and tissue engineering, delivery of therapeutics
In addition, BME research efforts include interdisciplinary activities, such as materials science and nano- and microelectronics, as well as computer science and applied mathematics.

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In that sense, BME is a very good example of the close multidisciplinary collaboration between the Center for Biosystems and the two other centers in BioSense Institute: Center for sensing technologies and Center for information technologies.

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Projects:

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H2020 MSCA-RISE IPANEMA GA 872662 - Integration of PAper-based Nucleic acid testing mEthods into Microfluidic devices for improved biosensing Applications

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<img class="alignnone wp-image-1421 size-full" src="https://api.biosens.rs/wp-content/uploads/2021/06/bs6.jpg" alt="" width="1100" height="624" />

Biomolecular engineering

IPANEMA – Integration of PAper-based Nucleic acid testing mEthods into Microfluidic devices for improved biosensing Applications

Centar za Biosisteme

Centar za senzorske tehnologije

Cilj projekta: IPANEMA projekat okuplja široku mrežu institucija sa multidisciplinarnim ekspertizama sa ciljem da razvije i primeni u praksi potencijal mikrofluidičnih platformi zasnovanih na papiru.
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O projektu: Nukleinske kiseline (uključujući deoksiribonukleinsku kiselinu ili DNK i ribonukleinsku kiselinu ili RNK) nalaze se u svim ćelijama i virusima. Testovi koji koriste amplifikaciju tj. umnožavanje nukleinske kiseline sve se više koriste pri detekciji DNK ili RNK povezane sa bakterijama, virusima ili drugim patogenima, čak i kada su prisutni u vrlo malim količinama. Međutim, takvi testovi (kao npr. PCR) su i dalje relativno komplikovani i skupi za široku upotrebu pri terenskom testiranju, bez odlaska u laboratoriju. Sa partnerima u Evropi, SAD, Kini, Rusiji i Izraelu, među kojima su i akademske institucije i kompanije, IPANEMA radi na nekoliko različitih tehnologija koje se mogu integrisati sa pametnim telefonima za sofisticiranu, ali isplativu i jednostavnu terensku dijagnostiku.
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 Viši naučni saradnik u oblasti prirodno-matematičke nauke - biologija

Dr Radonić je doktorirao iz oblasti elektronike 2010. godine na Univerzitetu u Novom Sadu. Autor je 2 poglavlja u knjizi, 30 radova u naučnim časopisima, više od 60 konferencijskih radova, 10 tehničkih rešenja i ima više od 440 citata. Dr Radonić je bio rukovodilac na četiri nacionalna projekta, jednom projektu koji je finansirala Evropska banka za istraživanje i razvoj, jednom bilateralnom projektu između Srbije i Slovenije, projektu koji je finansirao Good Food Institute iz SAD-a i vođa radnog paketa u jednom FP7 projektu – Innosens i jednom H2020 – Antares. Učestvovao je u brojnim istraživačkim projektima iz programa FP6, FP7, H2020, COST, Eureka, IPA. Član je grupa IEEE i MAGEO (Mikrotalasi u poljoprivredi, životnoj sredini i posmatranju Zemlje) i član tehničkih programskih odbora konferencije EUROCON (Computer as a Tool, 2015), Salamanka, Španija i Sensodevice konferencije 2019, Nica, Francuska. Član upravnog odbora Instituta BioSense od 2015-2020 i potpredsednik Naučnog veća Instituta BioSense 2015-2020. Od 2022 obavlja funkciju pomoćnika direktora za nauku. Recenzent u mnogim naučnim časopisima: Scientific Reports, Lab-on-Chip, IEEE Sensors Journal, MDPI Sensors, MDPI Electronics, Sensors and Actuators B, IEEE Wireless and component letters, Electronic letters, etc. Oblast naučnog istraživanja dr Radonića je mikrofluidika, razvoj senzora (mikrotalasni, optički, mikrofluidni senzori i biosenzori), elektronskih, RF i mikrotalasnih pasivnih komponenti (filteri, spojnica, diplekser itd.), antena i RFID sistema, periodični i veštački materijali, tehnologije fabrikacije, merenja i karakterizacija.

Stefan Jarić je zaposlen u Centru za senzorske tehnologije kao istraživač-saradnik i student završne godine doktorskih studija fizike materijala na Departmanu za fiziku, Prirodno-matematičkog fakulteta u Novom Sadu. Stefan se bavi razvojem i istraživanjem novih rešenja na polju biosenzora zasnovanih na grafenskim tranzistorima sa efektom polja i specifičnih sintetičkih receptora (aptamera) za detekciju raznih analita iz sfere zaštite životne sredine (toksini) i medicine (biomarkeri). Njegova ekpertiza se razvija u pravcu mikro i nanofabrikacije senzorskih platformi (fotolitografija, fizičko naparavanje materijala), karakterizacije materijala (Ramanova spektroskopija, mikroskopija atomskih sila) i električnih karakteristika biosenzora.

Slobodan Birgermajer je naučni saradnik u Centru za Senzorske Tehnologije pri Institutu BioSens. Doktorat iz mikrotalasne elektronike odbranio je 2019. godine na Fakultetu Tehničkoih Nauka, Univerziteta u Novom Sadu. Trenutno radi na razvoju i primeni senzora u poljprivredi, prehrambenoj industriji i monitoringu životne sredine, i aktivno je uključena na više međunarodnih projekata iz poziva H2020, kao što su ANTARES, SHEALTY, IPANEMA, i drugi. Takođe, domen istraživanja obuhvata razvoj i fabrikaciju mikrotalasnih pasivnih kola, fotonskih kristala, mikrofludinih senzora, kao i 3D i 4D tehnologiju štampe.

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Dr Nikola Knežević je Naučni savetnik u Centru za senzorske tehnologije na Institutu Biosens. Doktorirao je 2009. godine na Iowa State University, SAD, iz oblasti sinteze i funkcionalizacije poroznih silikatnih nanočestica za stimulans-responsivnu i ciljajuću isporuku lekova. Dalje istraživačko iskustvo stekao je kao postdoktorand na Univerzitetu u Hjustonu (istraživanje iz oblasti višestepene organske sinteze), Universidad Complutense de Madrid (nauka o nanomaterijalima), Institute Charles Gerhardt Montpellier (nauka o nanomaterijalima), FP7 – ERA ERA Chairs postdoktorand u Nuklearnom institutu Vinča i kao Pole Chimie Balard gostujući profesor na Univerzitetu u Monpeljeu. Nikola je autor/koautor na preko 40 naučnih publikacija, uključujući 2 poglavlja u knjigama i jedan patent, sa preko 1900 citata i H indeksom 21. Nikola je koordinator jednog projekta H2020 (NANOFACTS GA 952259), tri srpska nacionalna projekta (PRECAST – 6060755, dokaz koncepta – 5566, transfer tehnologija -1135) i dva bilateralna istraživačka projekta (sa Francuskom i Nemačkom). Istraživačka interesovanja: sinteza funkcionalnih bioresponsivnih nanomaterijala za primenu u zaštiti zdravlja i konstrukciji biosenzora.

Nejra Omerović je zaposlena na Institutu BioSens kao istraživač saradnik. Osnovne i master studije završila je 2015., odnosno 2017. godine na Tehnološkom fakultetu, Univerzitet u Novom Sadu, Srbija, gde je trenutno sudent doktorskih studija na Katedri za Inženjerstvo materijala. Od oktobra 2015. do septembra 2016. godine obavljala je stručnu praksu u kompaniji AGFA-Gevaert NV, Mortsel, Belgija, gde je radila na Departmanu za razvoj inkdžet mastila. Njene trenutne istraživačke aktivnosti usmerene su na biopolimere i njihove nanokompozite.

Minja Mladenović je zaposlena na Institutu BioSens kao istraživač-pripravnik. Takođe, student je doktorskih akademskih studija hemije na Prirodno-matematičkom fakultetu Univerziteta u Novom Sadu, gde je završila osnovne i master studije hemije. Bavi se naučno-istraživačkim radom u oblasti sinteze, funkcionalizacije i karakterizacije nanomaterijala na bazi silike, kao i primenom istih za stimulisanu i kontrolisanu isporuku lekova i biomolekula.

Dr Marko Radović je diplomirao 2005. na Fizičkom Fakultetu pri Beogradskom univerzitetu. 2008. je odbranio magistarski rad na Fizičkom Fakultetu na Univerzitetu u Beogradu. Doktorsku disertaciju je odbranio 2015. na Fizičkom Fakultetu u Beogradu. Od aprila 2006. je zaposlen na Institutu za Fiziku u Beogradu. Od februara 2015. je zaposlen kao naučni saradnik na Biosens Institutu. Naučno istraživačka delatnost Dr Radovića je fokusirana na tzv. “bottom-up approach” gde su povezane sinteza nanomaterijala, karakterizacija I funkcionalizacija nanomaterijala u dizajnu senzorskih optoelektronskih i fotokatalitičkih uređaja. Tokom dosadašnje karijere publikovao je više od 25 naučnih radova u međunarodnim i domaćim časopisima.

Jovana Stanojev je istraživač saradnik i student doktorskih studija na Tehnološkom fakultetu u Novom Sadu, na Univerzitetu u Novom Sadu. Studirala je inženjerstvo materijala i trenutno završava svoju doktorsku disertaciju. Tema njene doktorske disertacije je "Razvoj nehlađenog infracrvenog detektora na bazi olovo selenida". Njeno istraživanje se fokusira na razvoj novih materijala za senzore, nanomaterijala i nanomembrana, mikrofluidiku, uzgajanje ćelijskih kultura i razvoj platformi organ-na-čipu.

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Ivana Podunavac je istraživač pripravnik na Institutu BioSens i student doktorskih studija primenjene fizike na Prirodno-matematičkom fakultetu, Univerziteta u Novom Sadu. Njene oblasti istraživanja su mikrofluidika, kao i izrada i testiranje senzora.

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Mila Đisalov je diplomirani biohemičar i master molekularne biologije. Trenutno je student doktorskih studija biohemije na Prirodno-matematičkom fakultetu u Novom Sadu i istraživač-pripravnik na Institutu BioSens. Njena glavna istraživačka interesovanja vezana su za razvoj biosenzora i molekularnih alata za detekciju patogena, ćelijska poljoprivreda i razvoj laboratorija na čipu. Mila je takođe jedan od osnivača studentske grupe The BioSense Alt. Protein Project, osnovane na Institutu BioSens.

Mila Đisalov

Ljiljana Janjušević PhD je naučni saradnik na BioSens institutu, Unirzitet u Novom Sadu, R. Srbija. Diplomske, master i doktorske studije završila je na Departmanu za biologiju i ekoloiju Prirodno-matmatičkog fakulteta Univerziteta u Novom Sadu. Tokom doktorskih studija fokus istraživanja je bio usmeren na ispitivanje biološke aktivnosti i hemijskog sastava makrogljiva kao i etarskih ulja nekih biljaka. Istraživanja na Institutu su usmerena na razvoj senzora i molekularnih tehnika za detekkciju patogena, biomoleularni inžnjering i ćelijsku poljoprivredu.

Dr Ivana Gađanski je viši naučni saradnik na Institutu Biosens, Univerzitet u Novom Sadu, Srbija. Diplomirala je na Biološkom fakultetu Univerziteta u Beogradu kao prva u klasi (2003). Doktorirala je iz neuronauka (ocena summa cum laude – usmena odbrana) na Georg-August Univerzitetu i Institutu za biofizičku hemiju Maks Plank, Getingen, Nemačka (2007) i radila na posledoktorskim studijama na Univerzitetu Ludvig-Maksimilijian, Minhen, Nemačka. Ivana se zatim posvetila oblasti bioinženjerstva i provela godinu dana kao Fulbrajt stipendista – gostujući istraživač na Univerzitetu Kolumbija, Njujork, SAD u Laboratoriji za matične ćelije i tkivno inženjerstvo (rukovodilac: prof. Gordana Vunjak-Novaković). U BioSensu radi na bioinženjerstvu u oblasti ćelijske poljoprivrede - razvija rešenja za kultivisano meso. Takođe radi na razvoju biosenzora za monitoring u oblasti poljoprivrede, biomedicine i zaštite životne sredine. Ivana je stipendista Instituta za dobru hranu i koordinator Horizont2020 projekata DRAGON GA 810755 i IPANEMA GA 872662.

Ovaj projekat je finansiran od strane Evropske Unije, iz programa za Istraživanje i Inovacije-  Horizont 2020, pod sporazumom o grantu broj 872662.
                   

Horizont 2020

Biomolekuli su molekuli su koji omogućavaju funkcionisanje živih organizama, uključujući DNK, RNK, proteine, peptide i konstelaciju malih molekula koji se sintetišu u ćelijama. Biomolekularno inženjerstvo (BMI) obuhvata ispitivanje i inženjerstvo svih ovih živih sistema. BMI istraživanja koriste tehnike i metode iz sintetičke biologije, sistemske biologije, biomedicinskih istraživanja i biotehnologije, biohemije i biofizike bioloških sistema i ćelijske biologije za kvantifikovanje i manipulisanje strukturom i funkcijom molekula i makromolekularnih sklopova za potencijalne primene u sledećim oblastima:

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· Biotehnologija: Biosenzorski i bioanalitički uređaji, tehnologije ćelijskih kultura sa omogućenom mikrofluidikom (npr. organi na čipu), razvoj i primena novih molekularnih alata detekcije patogena (primenljivo za procenu bezbednosti hrane)

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· Osnovni procesi živih sistema: interakcije patogena i domaćina, ćelijska i unutarćelijska organizacija

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· Bolesti ljudi i životinja: dizajn i inženjerstvo dijagnostičkih metodologija, ćelijsko i tkivno inženjerstvo
Pored toga, BMI istraživanja uključuju veoma interdisciplinarne aktivnosti, kao što su nauka o materijalima i nano- i mikroelektronika, kao i računarstvo i primenjena matematika. U tom smislu, BMI je vrlo dobar primer bliske multidisciplinarne saradnje između Centra za biosisteme i druga dva centra u okviru BioSens Instituta - Centra za senzorske tehnologije i Centra za informacione tehnologije.

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Projekti:

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H2020 MSCA-RISE IPANEMA GA 872662 - Integration of PAper-based Nucleic acid testing mEthods into Microfluidic devices for improved biosensing Applications

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<img class="alignnone wp-image-1421 size-full" src="https://api.biosens.rs/wp-content/uploads/2021/06/bs6.jpg" alt="" width="1100" height="624" />

Biomolekularno inženjerstvo

Biosenzori su analitički uređaji koji služe za detekciju biološkog materijala kao što su biomolekuli, biološke strukture ili mikroorganizmi (bakterije, virusi, alge, spore). Široku primenu biosenzori nalaze u kontroli bezbednosti hrane, monitoringu životne sredine, kao i u ranom otkrivanju infektivnih bolesti u biomedicini.

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Naša istraživanja su usmerena na razvoj novih biosenzorskih transdjusera, biofunkcionalizaciju senzora sa bioreceptorima, i integraciju senzora u mikrofluidične sisteme. Razvijeni senzori se koriste za detekciju bakterija u hrani i vodi, za detekciju cijano toksina nastalih tokom cvetanja algi, kao i za brzu detekciju fekalnih bakterija i drugih mikrobioloških patogena u vodi.

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Bavimo se i razvojem novih nanomaterijala za funkcionalizaciju biosenzorskih elektroda i fabrikaciju novih elektrohemijskih biosenzora koristeći različite tehnologije fabrikacije. Deo istraživanja je usmeren na nove materijale koji će omogućiti selektivno i efikasno vezivanje analita, kao i na projektovanje biosenzora u cilju poboljšanja njihove selektivnosti, osetljivosti i limita detekcije. Bavimo se i novim metodama modifikacije grafena, novim metodama za njegovu sintezu i integraciju u konvencionalne industrijske procese.

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Posebna pažnja je posvećena biosenzorima na bazi enzima, antitela i nukleinskih kiselina (DNK, RNK). Ova istraživanja se izvode u bliskoj saradnji sa Centrom za biosisteme u kojem istraživači rade na metodama biomolekularnog inženjerstva i molekularne dijagnostike što uključuje i najsavremenije izotermalne metode za umnožavanje nukleinskih kiselina kao što su LAMP (Loop Mediated Isothermal Amplification) i RPA (Recombinase Polymerase Amplification) koje se mogu dobro kombinovati sa biosenzorima razvijenim u okviru Centra za senzorske tehnologije.

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Takođe, Institut BioSens radi na novim vrstama biosenzora za monitoring hranljivih materija i produkata metabolizma ćelija u procesu proizvodnje kultivisanog mesa, što je još jedan primer dobre multidisciplinarne saradnje sa Centrom za biosisteme i istraživačima koji rade u okviru oblasti ćelijske poljoprivrede.

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<img class="alignnone wp-image-1508 " src="https://api.biosens.rs/wp-content/uploads/2021/06/58.png" alt="" width="701" height="417" />

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Zajednički projekti sa Centrom za biosisteme:

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H2020 MSCA-RISE IPANEMA GA 872662 - Integration of PAper-based Nucleic acid testing mEthods into Microfluidic devices for improved biosensing Applications

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REALSENSE1 - Monitoring of cell culture parameters using sensors for biomass and nutrients/metabolites in media: Lab-on-a-Chip (LOC) approach, 2019-2021; Good Food Institute Inaugural Competitive Grant Program.

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REALSENSE2 – From lab-on-a-chip to custom bioreactor: scale up modeling study, 2020-2022 Good Food Institute Competitive Grant Program

Biosenzori

Mikrofluidika je oblast koja se bavi manipulacijom i kontrolom malih količina fluida u mikro- i nano-kanalima i kao takva mikrofludika je napravila pravu malu revoluciju u mikrobiološkim, medincinskim i hemijskim analizama.

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Naša istraživanja su fokusirana na analizu i projektovanje različitih mikrofludičinih komponenta, razvoj novih materijala za mikrofluidične čipove i integraciju mikrofluidike sa različitim sistemima za detekciju i elektronikom. U tu svrhu koristimo različite tehnologije fabrikacije, kao što su Polidimetilsiloksan (PDMS), niskotemperaturna zajedno pečena keramika (LTCC), 3D, inkdžet i bio-štampa, ksirografija, kao i hibridne tehnologije.
Kako korišćenje samo jedne tehnologije fabrikacije ne može da ispuni sve zahteve savremenih mikrofluidičnih sistema, koristimo hibridni pristup - kombinujemo različite tehnologije sa različitim materijalima da bismo dobili mikrofluidične čipove sa visokom rezolucijom, malim dimenzijama i visokim protocima. Na primer, hibridni pristup se koristi za razvoj višeslojnih čipova za filtraciju, mešača fluida za visoke protoke, kao i separatora za polen.

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Takođe, integrišemo mikrofluidne čipove sa reagentima za analizu na čipu sa različitim tipovima senzora u cilju razvoja labratorije na čipu za detekciju parametara fluida, koji nalaze primenu u kontroli mešanja fluida, biomedicine, monitoringu ekosistema i analizi tečne hrane.
Osim toga, radimo na razvoju mikrofluidičnog reaktora, koji integriše sistem za ćelijsku kulturu, različite senzore za detekciju koncentracije hranljivih materija i merenje metaboličkih otpadnih proizvoda, optičku detekciju i elektroniku u jedan jedinstveni sistem na čipu.

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Prateći ovaj koncept, razvijamo i mikrofluidičnu platformu koja ima mogućnost da reprodukuje dinamiku i heterogenost zemljišta na taj način razvijajući sistem za monitoring interakcije između biljke i mikrobioma.

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<img class="alignnone wp-image-1529 size-full" src="https://api.biosens.rs/wp-content/uploads/2021/06/70.jpg" alt="" width="763" height="323" />

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PROJEKTI:

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CISTEM - Heart On chip based on induced pluripotent Stem cell Technology for personalized Medicine, H2020-MSCA-RISE-2017, GA No. 778354

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REALSENSE1 - Monitoring of cell culture parameters using sensors for biomass and nutrients/metabolites in media: Lab-on-a-Chip (LOC) approach, 2019-2021; Good Food Institute Inaugural Competitive Grant Program.

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IPANEMA - Integration of PAper-based Nucleic acid testing mEthods into Microfluidic devices for improved biosensing Applications, H2020-MSCA-RISE-2019, 2020-2024. GA No. 872662,

Mikrofluidika

Zbog malog prečnika (oko 100 nm) i veoma porozne prirode, mezoporozne silikatne nanočestice (MSN) imaju veliku specifičnu površinu (oko 1000 m2 / g) koja se može koristiti za različite funkcionalizacije, punjenje/adsorpciju različitih molekula i njihovo uklanjanje, ili kao platforma za signalne molekule za detekciju različitih analita.

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Mezoporozni organosilikatni nanomaterijal (Periodični mezoporozni organosilikat (PMO)) sadrži veliki procenat organskih grupa, što omogućuje da ispoljavaju jedinstvene karakteristike za različite primene.

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Naše istraživanje se provodi u cilju dizajniranja novih serija MSN i PMO nanomaterijala, kao i njihovih jezgro / ljuska analoga.

Površina ovih nanomaterijala se modifikuje različitim molekulima kako bi specifično stupila u interakciju sa željenim tkivima, zagađivačima ili analitima, radi njihove efikasne primene u praćenju i zaštiti zdravlja, hrane i životne sredine.

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Jedan od ciljeva istraživanja je razvoj pametnih nanomaterijala sa stimulans-responzivnim oslobađanjem molekula iz MSN / PMO materijala, sa primenom u dijagnostici, snimanju i terapiji tkiva raka.
Kompozitni materijali polimer-MSN se takođe razvijaju u cilju primene kao antimikrobnih pametnih ambalaža za hranu i medicinskih implanata.

Konačno, nove PMO nanočestice se konstruišu radi efikasnog blokiranja UVA i UVB zračenja i primene u bezbednijoj generaciji krema za sunčanje.

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<img class="alignnone wp-image-1626 size-full" src="https://api.biosens.rs/wp-content/uploads/2021/06/slika-1.png" alt="" width="837" height="466" />

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Projekti:

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PRECAST: Prediction of Cancer Treatment Effectiveness with Stimuli-responsive Nanomaterials, Science Fund of the Republic of Serbia

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<a href="https://cordis.europa.eu/project/id/952259">NANOFACTS: Networking Activities for Nanotechnology-Facilitated Cancer Theranostics H2020-WIDESPREAD-2020-5</a>

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Novel Active Components for Health- and Enviroment-friendly UV-Blocking Based on Enriched Organosilica Nanomaterials, Project ID: 5566, The Innovation Fund of the Republic of Serbia

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III 44006 Razvoj novih informaciono-komunikacionih tehnologija, korišćenjem naprednih matematičkih metoda, sa primenama u medicini, telekomunikacijama, energetici, zaštiti nacionalne baštine i obrazovanju, Ministarstvo prosvete, nauke i tehnološkog razvoja Republike Srbije.

IPANEMA – Integration of PAper-based Nucleic acid testing mEthods into Microfluidic devices for improved biosensing Applications

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